6533b7dafe1ef96bd126ebf5
RESEARCH PRODUCT
NQS-Doped PDMS Solid Sensor: From Water Matrix to Urine Enzymatic Application
Ana Ballester-caudetYolanda Moliner-martínezCarmen Molins-leguaL. HakobyanPilar Campíns-falcóAdria Martínez-aviñosubject
In situAnalyteMaterials scienceUreasePolymersClinical BiochemistrywaterNQS02 engineering and technologyureaurea hydrolysis01 natural sciencesArticlechemistry.chemical_compoundDimethylpolysiloxanesoptical sensorureaseglass supportChromatographyPolydimethylsiloxanebiology010405 organic chemistryGeneral Medicine021001 nanoscience & nanotechnologyurine0104 chemical sciencesin-situ analysisammoniumMembranechemistryReagentUreabiology.proteinColorimetry0210 nano-technologyTP248.13-248.65NaphthoquinonesNQS-PDMS sensorBiotechnologydescription
The development of in situ analytical devices has gained outstanding scientific interest. A solid sensing membrane composed of 1,2-naphthoquinone-4-sulfonate (NQS) derivatizing reagent embedded into a polymeric polydimethylsiloxane (PDMS) composite was proposed for in situ ammonium (NH4+) and urea (NH2CONH2) analysis in water and urine samples, respectively. Satisfactory strategies were also applied for urease-catalyzed hydrolysis of urea, either in solution or glass-supported urease immobilization. Using diffuse reflectance measurements combined with digital image processing of color intensity (RGB coordinates), qualitative and quantitative analyte detection was assessed after the colorimetric reaction took place inside the sensing membrane. A suitable linear relationship was found between the sensor response and analyte concentration, and the results were validated by a thymol-PDMS-based sensor based on the Berthelot reaction. The suggested sensing device offers advantages such as rapidity, versatility, portability, and employment of non-toxic reagents that facilitate in situ analysis in an energy-efficient manner.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-06-01 | Biosensors |