Search results for "ample"

A Method for Isolation of the Virome from Plasma Samples

2018

Virome studies are of special interest nowadays. Understanding viral communities in different body compartments will help guide future personalized treatments and to discern between homeostasis and disease. High-throughput sequencing technologies allow us to detect all the nucleic acids present in a sample, including viral ones, by random sequencing. One of the major challenges in virome studies is the correct isolation of the viral nucleic acids from a specific sample. This can be done during the extraction steps (e.g., enrichment of viral capsids), or during the bioinformatic analysis (e.g., removing all human and bacterial sequences). Furthermore, it is an important remark that the treat…

Label-Free Proteomics of Quantity-Limited Samples Using Ion Mobility-Assisted Data-Independent Acquisition Mass Spectrometry

2021

Over the past two decades, unbiased data-independent acquisition (DIA) approaches have gained increasing popularity in the bottom-up proteomics field. Here, we describe an ion mobility separation enhanced DIA workflow for large-scale label-free quantitative proteomics studies where starting material is limited. We set a special focus on the single pot solid-phase-enhanced sample preparation (SP3) protocol, which is well suited for the processing of quantity-limited samples.

Do vegetarians feel bad? Examining the association between eating vegetarian and subjective well-being in two representative samples

2020

Abstract Research on the relationship between vegetarianism and subjective well-being (SWB) has produced inconsistent results, which may partly be due to small sample sizes and divergent operationalizations of well-being. For these reasons, the present study aimed to thoroughly examine this association in two large representative samples from Germany (Study 1: N = 12,905, including 665 vegetarians) and Australia (Study 2: N = 15,532, including 383 vegetarians) using a consensual conceptualization of SWB (composed of an affective component, i.e., positive and negative affect, and a cognitive component, i.e., life satisfaction). Results of t-tests showed that vegetarians reported slightly hig…

Building an Optimal WSD Ensemble Using Per-Word Selection of Best System

2006

In Senseval workshops for evaluating WSD systems [1,4,9], no one system or system type (classifier algorithm, type of system ensemble, extracted feature set, lexical knowledge source etc.) has been discovered that resolves all ambiguous words into their senses in a superior way. This paper presents a novel method for selecting the best system for target word based on readily available word features (number of senses, average amount of training per sense, dominant sense ratio). Applied to Senseval-3 and Senseval-2 English lexical sample state-of-art systems, a net gain of approximately 2.5 – 5.0% (respectively) in average precision per word over the best base system is achieved. The method c…

Oxidative stability of virgin olive oil: evaluation and prediction with an adaptive neuro-fuzzy inference system (ANFIS).

2019

Background An adaptive neuro-fuzzy inference system (ANFIS) was employed to predict the oxidative stability of virgin olive oil (VOO) during storage as a function of time, storage temperature, total polyphenol, α-tocopherol, fatty acid profile, ultraviolet (UV) extinction coefficient (K268 ), and diacylglycerols (DAGs). Results The mean total quantities of polyphenols and DAGs were 1.1 and 1.9 times lower in VOOs stored at 25 °C than in the initial samples, and the mean total quantities of polyphenols and DAGs were 1.3 and 2.26 times lower in VOOs stored at 37 °C than in the initial samples, respectively. In a single sample, α-tocopherol was reduced by between 0.52 and 0.91 times during sto…

Altruistic leadership and affiliative humor's role on service innovation: Lessons from Spanish public hospitals.

2018

Despite literature identifies aspects that might promote innovation, the relationship between the leadership style and nurses' innovative behavior still remains unclear, and little research has provided evidence of this. To help advance in knowledge of effects of leadership on nurses' innovative behavior, we researched the effect of altruistic leadership on nurses' innovative behavior. In addition, the mediating role of affiliative humor in the relationship between altruistic leadership and nurses' innovative behavior was examined. Questionnaire survey method was followed with a sample of 324 nurses working in public hospitals in Spain. We used structural equation models, to check the resea…

Double validation of translated questionnaires in international studies

2018

Background Study questionnaires developed in one country can be translated and used for similar studies in other countries. The main problem of this process is that people speaking different languages can understand the meaning of some questions differently due to disparate mentalities and backgrounds. To avoid a misunderstanding, to perform correct conclusions in accordance to the mentality of each country, and to permit the comparison of results in different countries, the intellectual validation of questionnaires is widely used. The main problem of such a validation is its qualitative nature that can be biased by the subjective approach of the investigator. Therefore, there is a necessit…

2019

Abstract This paper examines the relationship between health endowment and later-life outcomes in the labour market. The analysis is based on reduced-form models in which labour market outcomes are regressed on genetic variants related to the increased risk of cardiovascular diseases. We use linked Finnish data that have many strengths. Genetic risk scores constitute exogenous measures for health endowment, and accurate administrative tax records on earnings, employment and social income transfers provide a comprehensive account of an individual’s long-term performance in the labour market. The results show that although the direction of an effect is generally consistent with theoretical re…

Sexting Prevalence and Socio-Demographic Correlates in Spanish Secondary School Students

2020

Introduction This research analyzes the prevalence of sexting and socio-demographic correlates in a sample of 647 adolescents administered a questionnaire at secondary schools in Valencia (Spain). Methods The questionnaire results indicate that 61% of respondents reported being involved in at least one case of sexting, with 24, 58, and 18% reporting having sent a sext, received a sext, and forwarded a sext, respectively. Results More males and older adolescents reported having received and forwarded sexts than female and younger adolescents. Conclusions Furthermore, time spent using Information and Communications Technology (ICT) devices, use and frequency of use of social media, grade repe…

Motivations for Business Start-up: Are There any Differences Between Disabled and Non-disabled Microfinance Clients?

2015

We use an Ecuadorian sample to investigate if there are differences in motivations for business start-up between persons with and without disabilities. Generally, we do not document significant differences. The reason might be that we use a sample selected among customers of the microfinance bank Banco D-MIRO. Without targeted incentives, disabled microfinance customers must resemble non-disabled customers. Copyright © 2015 John Wiley & Sons, Ltd.