Search results for "anger"
showing 10 items of 929 documents
Is ABA involved in tolerance responses to salinity by affecting cytoplasm ion homeostasis in rice cell lines?
2012
Abstract The ability of plant cells to maintain cytoplasm ion homeostasis under saline stress is among the main mechanisms involved in salt tolerance. To cope with excess Na + , cells extrude it from the cytoplasm, which requires expenditure of metabolic energy, provided by H + gradients generated by membrane-bound H + -pumps. ABA is well-known to be involved in physiological processes elicited or enhanced by stresses causing cell dehydration. In this work we studied the possible implication of this plant hormone in the control of salt-induced cellular mechanisms conducting to Na + extrusion from the cytoplasm. We used rice ( Oryza sativa L.) cell lines selected for their different toleranc…
Mechanisms of endothelial cell swelling from lactacidosis studied in vitro
2000
One of the early sequelae of ischemia is an increase of circulating lactic acid that occurs in response to anaerobic metabolism. The purpose of the present study was to investigate whether lactic acidosis can induce endothelial swelling in vitro under closely controlled extracellular conditions. Cell volume of suspended cultured bovine aortic endothelial cells was measured by use of an advanced Coulter technique employing the “pulse area analysis” signal-processing technique (CASY1). The isosmotic reduction of pH from 7.4 to 6.8 had no effect on cell volume. Lowering of pH to 6.6, 6.4, or 6.0, however, led to significant, pH-dependent increases of cell volume. Swelling was more pronounced …
Flow cytometric kinetic assay of the activity of Na+/H+ antiporter in mammalian cells.
2004
Background The Na+/H+ exchanger (NHE) of mammalian cells is an integral membrane protein that extrudes H+ ion in exchange for extracellular Na+ and plays a crucial role in the regulation of intracellular pH (pHi). Thus, when pHi is lowered, NHE extrudes protons at a rate depending of pHi that can be expressed as pH units/s. Methods To abolish the activity of other cellular pH-restoring systems, cells were incubated in bicarbonate-free Dulbecco's modified Eagle's medium buffered with HEPES. Flow cytometry was used to determine pHi with 2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester or 5-(and-6)-carboxy SNARF-1 acetoxymethyl ester acetate, and the appropriate fluo…
The search for identity in the narrative stylistic and poetic study
2016
This dissertation deals with the quest for narrative identity in four distinct narratives: Le Premier Homme, by Albert Camus; Les Oliviers de la justice, by Jean Pélégri; Ébauche du père, by Jean Sénac; and Outremer, by Morgan Sportes. The authors of these narratives belonged to the community of French people born in Algeria and, later on, to that of the pieds-noirs. The texts examine mainly the end of French Algeria. This accounts for the reference to the years 1954-1962, among other periods of time, and to the tragic consequences these years led to, including exile and uprooting. The designation “French Algeria” in itself contains at least a dual reference to identity, if not referring to…
Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography
1989
Abstract The linear solvent strength model of Snyder was applied to describe fast protein separations on 2.1-μm non-porous, silica-based strong anion exchangers. It was demonstrated on short columns packed with these anion exchangers that (i) a substantially higher resolution of proteins and nucleotides was obtained at gradient times of less than 5 min than on porous anion exchangers; (ii) the low external surface area of the non-porous anion exchanger is not a critical parameter in analytical separations and (iii) μg-amounts of enzymes of high purity and full biological activity were isolated.
Cutaneous Exposure to the Superantigen Staphylococcal Enterotoxin B Elicits a T-Cell-Dependent Inflammatory Response
1996
We analyzed the impact of superantigens secreted by skin-colonizing Staphylococci on the skin and the associated lymphoid tissue following epicutaneous application and intracutaneous injection of small amounts of staphylococcal enterotoxin B (SEB). A single intracutaneous injection of 50 ng of SEB elicited a strong inflammatory response in the skin of BALB/c mice. Three to 6 h later, we observed langerhans cell activation, mast cell degranulation, vasodilation, upregulation of ICAM-1, and induction of VCAM-1 on dermal blood vessels, with vascular adhesion of granulocytes. by 12 to 24 h, cell infiltration of the dermis increased, reaching the epidermis. Among the infiltrating leukocytes, a s…
Estimating the decomposition of predictive information in multivariate systems
2015
In the study of complex systems from observed multivariate time series, insight into the evolution of one system may be under investigation, which can be explained by the information storage of the system and the information transfer from other interacting systems. We present a framework for the model-free estimation of information storage and information transfer computed as the terms composing the predictive information about the target of a multivariate dynamical process. The approach tackles the curse of dimensionality employing a nonuniform embedding scheme that selects progressively, among the past components of the multivariate process, only those that contribute most, in terms of co…
Financial distress and real economic activity in Lithuania: a Granger causality test based on mixed-frequency VAR
2020
In this paper, we extend the monthly financial stress index for Lithuania, computed by the European Central Bank, to a daily frequency and we also include banking sector stress among its constituents, beyond bond, equity and foreign exchange markets. We investigate the causal relationship between the daily financial stress index and monthly industrial production growth, using a Granger causality test applied to a mixed-frequency VAR. Our results suggest evidence of Granger causality from financial stress to industrial production growth once the index is enriched by daily observations from the financial markets. Our findings, based on impulse response analysis, confirm the negative effect of…
Multiscale Granger causality
2017
In the study of complex physical and biological systems represented by multivariate stochastic processes, an issue of great relevance is the description of the system dynamics spanning multiple temporal scales. While methods to assess the dynamic complexity of individual processes at different time scales are well-established, multiscale analysis of directed interactions has never been formalized theoretically, and empirical evaluations are complicated by practical issues such as filtering and downsampling. Here we extend the very popular measure of Granger causality (GC), a prominent tool for assessing directed lagged interactions between joint processes, to quantify information transfer a…
FROM PRODUCER TO CONSUMER – RELATIONS BETWEEN PRICES OF SELECTED PRODUCTS ON THE AGRI-FOOD MARKET
2019
The food supply chain is characterized by a large diversity of entities comprising it, combining actions taken by individual links, starting with the producer on the consumer. The primary goal of the smooth functioning of the food supply chain is to ensure satisfaction of buyers, while profiting by companies participating in the flow of products. The primary aim of the efficient functioning of the food supply chain is to ensure satisfaction of buyers, while profiting by companies participating in the flow of products. Changes occurring in agricultural production, often cyclical, are transferred to individual links in the supply chain. This phenomenon is visible in changes in the level of pr…