Search results for "api"

Looking into the architecture of the brain with MRI: quantification of non-Gaussian water diffusion by Diffusion Kurtosis Imaging (DKI)

2015

The aim of this work is the definition of an MRI protocol for Diffusion Kurtosis Imaging (DKI) by using a 1.5T clinical scanner and the development of a software for DKI analysis.

Single molecule localization microscopy of the distribution of chromatin using Hoechst and DAPI fluorescent probes.

2014

Several approaches have been described to fluorescently label and image DNA and chromatin in situ on the single-molecule level. These superresolution microscopy techniques are based on detecting optically isolated, fluorescently tagged anti-histone antibodies, fluorescently labeled DNA precursor analogs, or fluorescent dyes bound to DNA. Presently they suffer from various drawbacks such as low labeling efficiency or interference with DNA structure. In this report, we demonstrate that DNA minor groove binding dyes, such as Hoechst 33258, Hoechst 33342, and DAPI, can be effectively employed in single molecule localization microscopy (SMLM) with high optical and structural resolution. Upon ill…

Selective, Highly Sensitive, and Rapid Detection of Genomic DNA by Using Gated Materials:MycoplasmaDetection

2013

The coupling of gated-indicator delivery with highly specific biochemical recognition is an innovative strategy for the detection of DNA sequences, able to compete with classical methods which need PCR amplification, in important areas such as point-of-care diagnostics or detection of specific biological contaminations with pathogens. Such comparatively simple and cheap yet highly selective and sensitive assays hold promise for use in less-developed areas of the world.

Characterization of a cDNA encoding RP43, a CUB-domain-containing protein from the tube of Riftia pachyptila (Vestimentifera), and distribution of it…

2000

A major 43kDa protein from the protective tube of Riftiapachyptila (Vestimentifera), named RP43, was partly microsequenced after isolation by SDS/PAGE from the protein fraction of tubes collected around the hydrothermal vents at the East Pacific Rise. On the basis of the partial peptide sequences obtained, experiments using reverse-transcriptase-mediated PCR and rapid amplification of cDNA ends led to the complete cDNA sequence. Analysis of deduced amino acid sequence of RP43 showed the presence of CUB domains (100–110-residue-spanning domains first reported in the complement subcomponents C1r/C1s, epidermal-growth-factor-related sea urchin protein and bone morphogenetic protein 1) that se…

Molecular cloning of rat G-protein-coupled receptor kinase 6 (GRK6) from brain tissue, and its mRNA expression in different brain regions and periphe…

1997

The rat G-protein-coupled receptor kinase 6 (GRK6) cDNA was cloned from rat brain tissue by a combination of reverse-transcription polymerase chain reactions (RT-PCR), based on homology to the cloned human GRK6, and rapid amplification of cDNA ends (RACE-PCR). We obtained a clone of 2817 bp with an open reading frame of 1731 bp encoding for a protein of 576 amino acids that is 96.7% identical and 97.9% similar to its human counterpart. mRNA was detectable in all brain areas examined. In addition, GRK6 was expressed in skeletal muscle, small intestine, aorta, liver, heart, lung, thymus, stomach, uterus and kidney.

Divergent Distribution in Vascular and Avascular Mammalian Retinae Links Neuroglobin to Cellular Respiration

2005

The visual function of the vertebrate retina relies on sufficient supply with oxygen. Neuroglobin is a respiratory protein thought to play an essential role in oxygen homeostasis of neuronal cells. For further understanding of its function, we compared the distribution of neuroglobin and mitochondria in both vascular and avascular mammalian retinae. In the vascular retinae of mouse and rat, oxygen is supplied by the outer choroidal, deep retinal, and inner capillaries. We show that in this type of retina, mitochondria are concentrated in the inner segments of photoreceptor cells, the outer and the inner plexiform layers, and the ganglion cell layer. These are the same regions in which oxyge…

IL CAPITALE RELAZIONALE NEI SISTEMI PRODUTTIVI DELLA DOCG BRUNELLO DI MONTALCINO E DELLA DOC ALCAMO

2012

The Xícaras from Don Hilarion: the public spaces and private homes in the eighteenth century rural

2012

El inventario post mortem y el libro de cuenta y razón del escribano Hilarión Cardona nos proporcionan una abundante información sobre las formas de vida y las actividades de este singular personaje y de su entorno. A su ascenso económico y social ayudará una estudiada política matrimonial, lo que le permitirá hacerse un hueco destacado entre los miembros de la oligarquía local. Lo nutrido y excepcional de su vestuario, junto con la posesión de novedosos objetos, nada comunes al resto de los inventarios de bienes, nos ofrecen una clara imagen del proceso de trasferencia de las formas de vida entre la ciudad y el medio rural. Hilarión sintetiza la emulación de los hábitos de comportamiento d…

Troksnis kā pacienta un personāla veselību ietekmējošs faktors intensīvās terapijas nodaļā

2015

Maģistratūras darba tēma – Troksnis kā pacientu un personāla veselību ietekmējošs faktors intensīvās terapijas nodaļā. Tēmas aktualitāti nosaka tas, ka troksnis ir nozīmīga vides problēma. Intensīvā terapija ir viena no medicīnas nozarēm, kurā strādājošajiem ir paaugstināta iespēja sastapties ar troksni kā darba vides riska faktoru. Darba mērķis ir aprakstīt un analizēt troksni kā faktoru, kas ietekmē pacienta un personāla veselību intensīvās terapijas nodaļā. Maģistra darbs sastāv no teorētiskās daļas un pētnieciskās daļas. Teorētiskajā daļā tika analizēti un apkopoti 50 literatūras avoti par troksni, trokšņa ietekmi uz fizioloģiju, psihiskām un sociālām problēmām, trokšņu īpatnībām, trokš…

Diffusion tensor imaging in amyotrphic lateral sclerosis: quantitative assesment ot the corticospinal tract degeneration.

2007

Poster for European Society of NeuroRadiology. Genoa, 20-23 September 2007