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Automated Determination of Ziprasidone by HPLC With Column Switching and Spectrophotometric Detection

2005

An isocratic high-performance liquid chromatography (HPLC) method with column switching and ultraviolet (UV) detection is described for quantitative analysis of the new antipsychotic drug ziprasidone. After centrifugation of serum or plasma samples and addition of fluperlapine as internal standard, the samples were injected into the HPLC system. On-line sample clean-up was conducted on a column (10 x 4.0 mm ID) filled with silica C8 material (20-microm particle size) using 8% (vol/vol) acetonitrile in deionized water as eluent. Ziprasidone was eluted and separated on ODS Hypersil C18 material (5 microm; column size 250 x 4.6 mm ID) using acetonitrile-water-tetramethylethylendiamine (50:49.6…

AdultMaleTime FactorsSensitivity and SpecificityHigh-performance liquid chromatographyDrug Administration SchedulePiperazinesAutomationBenzodiazepinesBlood serumColumn chromatographymedicineHumansPharmacology (medical)ZiprasidoneClozapineChromatography High Pressure LiquidPharmacologyDetection limitChromatographymedicine.diagnostic_testElutionChemistryReproducibility of ResultsMiddle AgedThiazolesOlanzapineSpectrophotometryTherapeutic drug monitoringSchizophreniaFemaleDrug MonitoringQuantitative analysis (chemistry)medicine.drugTherapeutic Drug Monitoring
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Conventional and spectral power analysis of all-night sleep EEG after subchronic treatment with paroxetine in healthy male volunteers.

1998

Paroxetine is a selective and potent serotonin reuptake inhibitor with reported antidepressant properties. Since changes in the regular sleeping pattern were described as side effects under treatment with paroxetine, the impact of the drug on the sleep architecture is of major interest. The present study addressed the question of subchronic effects of paroxetine medication (30 mg/day) in eight healthy male volunteers in a double blind, placebo-controlled crossover-design. Conventional sleep EEG parameters and additionally computed spectral power analysis based on FFT of 20-s time epochs in the delta, theta, alpha, beta and gamma frequency range for different sleep stages after 4 weeks of tr…

AdultMaleTime FactorsSerotonin reuptake inhibitorSleep REMNon-rapid eye movement sleepDouble-Blind MethodReference ValuesmedicineHumansPharmacology (medical)Biological PsychiatrySlow-wave sleepPharmacologySleep StagesAnalysis of VarianceCross-Over StudiesElectroencephalographySleep in non-human animalsParoxetineCircadian RhythmPsychiatry and Mental healthParoxetineNeurologyAnesthesiaAntidepressantAntidepressive Agents Second-GenerationNeurology (clinical)Sleep onset latencyPsychologySleepSelective Serotonin Reuptake Inhibitorsmedicine.drugEuropean neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology
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Autosomal Recessive Hypercholesterolemia Long-Term Cardiovascular Outcomes

2017

BACKGROUND Autosomal recessive hypercholesterolemia (ARH) is a rare lipid disorder characterized by premature atherosclerotic cardiovascular disease (ASCVD). There are sparse data for clinical management and cardiovascular outcomes in ARH. OBJECTIVES Evaluation of changes in lipid management, achievement of low-density lipoprotein cholesterol (LDL-C) goals and cardiovascular outcomes in ARH. METHODS Published ARH cases were identified by electronic search. All corresponding authors and physicians known to treat these patients were asked to provide follow-up information, using a standardized protocol. RESULTS We collected data for 52 patients (28 females, 24 males; 31.1 +/- 17.1 years of age…

AdultMaleTime FactorsSettore MED/09 - Medicina InternaAdolescentatherosclerotic cardiovascular disease; autosomal recessive hypercholesterolemia; follow-up; lipid-lowering therapies; retrospective analysisHypercholesterolemiaretrospective analysiatherosclerotic cardiovascular disease; autosomal recessive hypercholesterolemia; follow-up; lipid-lowering therapies; retrospective analysis; Cardiology and Cardiovascular MedicineCohort StudiesYoung Adultautosomal recessive hypercholesterolemialipid-lowering therapiesfollow-upHumansLongitudinal StudiesChildlipid-lowering therapiesAgedRetrospective Studieslipid-lowering therapieatherosclerotic cardiovascular diseaseCholesterol LDLMiddle Agedretrospective analysisTreatment OutcomeCardiovascular DiseasesChild PreschoolFemaleCardiology and Cardiovascular MedicineFollow-Up Studies
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A retrospective study of the prevalence and characteristics of dens invaginatus in a sample of the Turkish population.

2012

Objective: The purpose of this study was to assess the prevalence of dens invaginatus and to classify the types of dens invaginatus in a sample of the Turkish population.. Study Design: A retrospective study was performed using periapical and panoramic radiographs of 5355 patients who presented to the Department of Oral Diagnosis and Radiology at the Ondokuz Mayıs University Dentistry Faculty between January 2009 and December 2010. Maxillary and mandibular anterior teeth were evaluated for the presence and characteristics of dens invaginatus. Statistical evaluation of the presence of dens invaginatus related to gender was performed by the Pearson chi-squared test. Results: Dens invaginatus …

AdultMaleTurkish populationAdolescentTurkeyvirusesTurkishLarge populationDentistryOdontologíadigestive systemcomplex mixturesDens invaginatusYoung Adultfluids and secretionsmedicinePrevalenceHumansDens invaginatusOral Diagnosisdens in denteGeneral DentistryAnterior teethAgedRetrospective StudiesPeriodontitisOral Medicine and Pathologybusiness.industryRetrospective cohort studydental anomalyMiddle Agedmedicine.disease:CIENCIAS MÉDICAS [UNESCO]Ciencias de la saluddigestive system diseasesPeriapical lesionDens in DenteOtorhinolaryngologyUNESCO::CIENCIAS MÉDICASSurgeryFemaleResearch-ArticlebusinessMedicina oral, patologia oral y cirugia bucal
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Efficacy of single-source rapid kV-switching dual-energy CT for characterization of non-uric acid renal stones: a prospective ex vivo study using ant…

2019

Purpose To investigate the accuracy of rapid kV-switching single-source dual-energy computed tomography (rsDECT) for prediction of classes of non-uric-acid stones. Materials and methods Non-uric-acid renal stones retrieved via percutaneous nephrolithotomy were prospectively collected between January 2017 and February 2018 in a single institution. Only stones >= 5 mm and with pure composition (i.e., >= 80% composed of one component) were included. Stone composition was determined using Fourier Transform Infrared Spectroscopy. The stones were scanned in 32-cm-wide anthropomorphic whole-body phantom using rsDECT. The effective atomic number (Zeff), the attenuation at 40 keV (HU40), 70 ke…

AdultMaleUrologymedicine.medical_treatmentStone compositionNephrolithotomy PercutaneousImaging phantom030218 nuclear medicine & medical imagingRapid kVp switchingRadiography Dual-Energy Scanned Projection03 medical and health scienceschemistry.chemical_compoundSingle-source0302 clinical medicineSpectroscopy Fourier Transform InfraredmedicineHumansUrolithiasiRadiology Nuclear Medicine and imagingProspective StudiesStone compositionKidney stonePercutaneous nephrolithotomyAgedRadiological and Ultrasound TechnologyPhantoms Imagingbusiness.industryGastroenterologyMiddle Agedmedicine.diseaseDual-energy CTchemistry030220 oncology & carcinogenesisMann–Whitney U testRadiographic Image Interpretation Computer-AssistedUric acidFemaleUrinary CalculiKidney stonesDual energy ctTomography X-Ray ComputedNuclear medicinebusinessSettore MED/36 - Diagnostica Per Immagini E RadioterapiaEffective atomic number
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Being in transit and in transition The experience of time at the place, when living with severe incurable disease - a phenomenological study

2014

The aim of this study is to describe the experience of time as it presents itself at the place being situated when living with severe incurable disease and receiving palliative care. The empirical data consist of 26 open-ended interviews with 23 patients receiving palliative care at home, at a palliative day care; in a palliative bed unite in hospital or in a nursing home in Norway. A common meaning of a shifting space for living emerged from the analysis and was revealed through three different aspects: (i) Transition from a predictable to an unpredictable time: To live with severe incurable disease marks a transition to a changed life involving an ongoing weakened and altered body with bo…

AdultMaleWeaknessPsychotherapistPalliative caremedia_common.quotation_subjectDay careAdult Day Care CentersExistentialismPhenomenology (philosophy)NursingSituatedmedicineHumansTerminally IllAgedmedia_commonAged 80 and overNorwayAtmospherePalliative CarePublic Health Environmental and Occupational HealthMiddle AgedHome Care ServicesEmbodiedNursing HomesPlace of careDistressFeelingTransitionPalliative careFemalePhenomenologyExperience of timemedicine.symptomPsychologyScandinavian Journal of Caring Sciences
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Tracing patterns of activity in the human skeleton: an overview of methods, problems, and limits of interpretation.

2009

Studies of patterns of activity in human skeletal remains have grown in number over the last few years. Different methods have been used to reconstruct activity patterns in past populations. In this review of the available literature the common themes of these studies have been isolated in order to show that many studies do not truly conform to the standards of the field. Inadequate sample size, too far-reaching conclusions and neglect of other possible explanations are among the problems easily recognised in the literature. Many assumptions are lacking a sound experimental basis, and it becomes increasingly evident that there are many more problems and limits of interpretations than have b…

AdultMaleWorkmedia_common.quotation_subjectTracingMotor ActivityWork physiologyBone and BonesNeglectAnthropology PhysicalBasic researchSexual division of labourmedicineHumansmedia_commonSex CharacteristicsBone DevelopmentInterpretation (philosophy)Adaptation PhysiologicalGenealogyEpistemologyHuman skeletonmedicine.anatomical_structureHomo sapiensAnthropologyFemalePsychologyHomo : internationale Zeitschrift fur die vergleichende Forschung am Menschen
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Novel rearrangements involving the RET gene in papillary thyroid carcinoma.

2018

Abstract Background In the field of gene fusions driving tumorigenesis in papillary thyroid carcinoma (PTC), rearrangement of the proto-oncogene RET is the most frequent alteration. Apart from the most common rearrangement of RET to CCDC6, more than 15 partner genes are yet reported. The landscape of RET rearrangements in PTC (“RET-PTC”) can notably be enlarged by modern targeted next-generation sequencing, indicating similarities between oncogenic pathways in other cancer types with identical genetic alterations. Methods Targeted next-generation sequencing was performed for two cases of BRAF-wild type PTC with confirmation of the results by Sanger sequencing. A “UniProt” database research …

AdultMalecongenital hereditary and neonatal diseases and abnormalitiesendocrine systemCancer Researchendocrine system diseasesOncogene Proteins FusionBiologyRUN domainmedicine.disease_causeProto-Oncogene MasFusion gene03 medical and health sciencessymbols.namesake0302 clinical medicineGeneticsmedicineHumansThyroid NeoplasmsneoplasmsMolecular BiologyGeneSanger sequencingGene RearrangementProto-Oncogene Proteins c-retIntracellular Signaling Peptides and ProteinsCancerHigh-Throughput Nucleotide SequencingNuclear ProteinsProtein-Tyrosine Kinasesmedicine.diseaseLisH domainThyroid Cancer Papillary030220 oncology & carcinogenesisCancer researchsymbolsFemaleCarcinogenesisCarrier ProteinsTyrosine kinaseCancer genetics
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Presence of human papillomavirus DNA in testicular biopsies from nonobstructive azoospermic men.

2005

Abstract Context.—Human papillomavirus (HPV) plays a major role in the etiology of many malignancies of diverse localization, such as uterine cervical carcinoma and its precursors. Human papillomavirus sequences have been detected throughout the male lower genitourinary tract, but the role of men as transmitters remains unclear. Objective.—To investigate the relationship between azoospermia and the presence of HPV DNA in testicular cells. Design.—One hundred eighty-five patients with azoospermia undergoing testicular biopsy were studied. Histologic study was done on formalin-fixed, paraffin-embedded samples from testicular biopsies, stained with hematoxylin-eosin. Molecular study to detect …

AdultMaleendocrine systemPathologymedicine.medical_specialtyStromal cellBiopsyBiologyPathology and Forensic MedicineBiopsyGenotypeTestismedicineHumansDNA Probes HPVPapillomaviridaeMicrodissectionAzoospermiamedicine.diagnostic_testurogenital systemGenitourinary systemHybridization probePapillomavirus InfectionsGeneral MedicineOligospermiaMiddle Agedmedicine.diseaseMedical Laboratory Technologygenomic DNADNA ViralArchives of pathologylaboratory medicine
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Detection of oncogenic human papillomavirus genotypes on spermatozoa from male partners of infertile couples

2013

Objective To evaluate the prevalence of human papillomavirus (HPV) sperm infection and its correlation with sperm parameters in patients who attended a fertility clinic. Design Cross-sectional clinical study. Setting University-affiliated reproductive medicine clinic. Patient(s) A total of 308 male partners of couples undergoing in vitro fertilization techniques. Intervention(s) Specimens of semen were collected from all patients. Main Outcome Measure(s) Sperm parameters were evaluated according to the World Health Organization manual. The presence of HPV DNA was researched by the combined use of two HPV assays and a highly sensitive nested polymerase chain reaction assay followed by HPV ge…

AdultMaleendocrine systemmedicine.medical_specialtyGenotypeReproductive medicineSemenFertilization in VitroBiologyPolymerase Chain ReactionHuman Papillomavirus DNA TestsMale infertilityAndrologySemen qualityPredictive Value of TestsRisk FactorsGenotypemedicineHumansPapillomaviridaeIn Situ HybridizationInfertility MaleAcademic Medical CentersSperm Counturogenital systemPapillomavirus InfectionsHPV infectionvirus diseasesObstetrics and Gynecologymedicine.diseaseSpermatozoaSpermSettore MED/40 - Ginecologia E Ostetriciafemale genital diseases and pregnancy complicationsFertilityReproductive MedicineCase-Control StudiesDNA ViralSperm MotilitySperm HeadHPV infection semen parameters IVF ICSINested polymerase chain reaction
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