Search results for "api"
showing 10 items of 8586 documents
Capitales ou villes d'appui ? Les petites villes et leurs campagnes du Moyen Age au XXIe siècle
2014
International audience
Conversaciones con Àngela Escribano
2021
En este mini-vídeo se entrevista a la Dra. Ángela Escribano, experta universitaria en violencia de género. El vídeo nos aporta pinceladas sobre cómo detectar y actuar en caso de que sospechemos que una de nuestras pacientes pueda estar sufriendo violencia de género.
Efectos de la i-logica capitalista en la violencia de genero
2015
Las autoras de este artículo reflexionan sobre los cierres y recortes en recursos de servicios sociales en general y de atención a las mujeres víctimas de violencia de género en particular, concluyendo que es la ideología mercantilista aplicada a los recursos sociales de atención a los más necesitados, la principal causante de que estos no funcionen adecuadamente y de que las mujeres víctimas de violencia en muchos casos no reciban la atención que seria necesaria.
Musiikin vaikutus ikääntyneiden asukkaiden hyvinvointiin kahdessa jyväskyläläisessä palvelutalossa
2010
Transfection of lipoma cells with papilloma bovine virus subgenomic fragment.
1991
Abstract Lipoma cells with consistent chromosomal aberration have been transfected with plasmids carrying papilloma bovine virus subgenomic fragment (PBV 69). The succesful transformation of the cells was ascerted on the changed growth pattern of the cells in liquid medium, colony formation in soft agar and modified cell appearrance in electron microscopy; transfection with PBV 69 has not been, however, sufficient to immortalize lipoma cells.
Internalization of novel non-viral vector TAT-streptavidin into human cells
2007
BMC Biotechnology, 7 (1)
Binding and internalization of human papillomavirus type 33 virus-like particles by eukaryotic cells
1995
Infection of cells by human papillomaviruses (HPVs) associated with malignant genital lesions has not been studied because of the lack of an in vitro system and the unavailability of virions. We have now used virus-like particles (VLPs) of HPV type 33 to analyze the initial events in the interaction of the HPV capsid with cell lines. Binding of VLPs to HeLa cells was observed in biochemical assays and by immunofluorescence. VLP binding was inhibited by antisera raised against VLPs but not by monoclonal antibodies recognizing either L1 or L2 epitopes accessible on VLPs. Under saturating conditions, approximately 2 x 10(4) VLPs were bound per cell, with a dissociation constant of about 100 pM…
Assembly and Translocation of Papillomavirus Capsid Proteins
2002
ABSTRACT The major and minor capsid proteins of polyomavirus are preassembled in the cytoplasm and translocated to the nucleus only as a VP1-VP2/VP3 complex. In this study, we describe independent nuclear translocation of the L1 major protein and the L2 minor capsid protein of human papillomavirus type 33 by several approaches. First, we observed that expression and nuclear translocation of L2 in natural lesions precede expression of L1. Second, using a cell culture system for coexpression, we found that accumulation of L2 in nuclear domain 10 (ND10) subnuclear structures precedes L1 by several hours. In contrast, complexes of L2 and mutants of L1 forced to assemble in the cytoplasm are tra…
Nuclear localization but not PML protein is required for incorporation of the papillomavirus minor capsid protein L2 into virus-like particles.
2004
ABSTRACT Recent reports suggest that nuclear domain(s) 10 (ND10) is the site of papillomavirus morphogenesis. The viral genome replicates in or close to ND10. In addition, the minor capsid protein, L2, accumulates in these subnuclear structures and recruits the major capsid protein, L1. We have now used cell lines deficient for promyelocytic leukemia (PML) protein, the main structural component of ND10, to study the role of this nuclear protein for L2 incorporation into virus-like particles (VLPs). L2 expressed in PML protein knockout (PML −/− ) cells accumulated in nuclear dots, which resemble L2 aggregates forming at ND10 in PML protein-containing cells. These L2 assemblies also attracted…
Generation and neutralization of pseudovirions of human papillomavirus type 33
1997
Since human papillomaviruses (HPV) cannot be propagated in cell culture, the generation of infectious virions in vitro is a highly desirable goal. Here we report that pseudovirions can be generated by the assembly of virus-like particles (VLPs) in COS-7 cells containing multiple copies of a marker plasmid. Using recombinant vaccinia viruses, we have obtained spherical VLPs of HPV type 33 (HPV-33) which fractionate into heavy and light VLPs in cesium chloride density gradients. VLPs in the heavy fraction (1.31 g/cm3) carry the plasmid in DNase-resistant form and are capable of transferring the genetic marker located on the plasmid to COS-7 cells in a DNase-resistant way (pseudoinfection). Th…