Search results for "aqueous humor"
showing 10 items of 25 documents
The ocular penetration of oral sparfloxacin in humans
1994
The penetration of sparfloxacin into the aqueous humor after oral administration was studied in 28 patients undergoing cataract surgery. Each patient received a single, oral dose of 400 mg of sparfloxacin. In eight other patients scheduled to undergo vitreal surgery, multiple daily oral doses were administered for a total amount of 1,000 mg. The aqueous levels were (mean +/- SEM) 0.127 +/- 0.036 microgram/ml to 0.404 +/- 0.159 microgram/ml from two to 24 hours after ingestion. In the vitreous, the mean drug level was 0.840 microgram/ml (range, 0.480 to 2.060 microgram/ml), from 4.3 to 8.0 hours after the most recent oral dose. Blood samples obtained at the same time as vitreous and aqueous …
CD4/CD8 Ratio in Aqueous Humor in Uveitis
2013
Dear Editor,We present a case series report with a simple and reproducible method for anterior uveitis investigation. This method uses the CD4/CD8 ratio in aqueous humor, analyzed by flow cytometry...
Altered antioxidant-oxidant status in the aqueous humor and peripheral blood of patients with retinitis pigmentosa.
2013
Retinitis Pigmentosa is a common form of hereditary retinal degeneration constituting the largest Mendelian genetic cause of blindness in the developed world. It has been widely suggested that oxidative stress possibly contributes to its pathogenesis. We measured the levels of total antioxidant capacity, free nitrotyrosine, thiobarbituric acid reactive substances (TBARS) formation, extracellular superoxide dismutase (SOD3) activity, protein, metabolites of the nitric oxide/cyclic GMP pathway, heme oxygenase-I and inducible nitric oxide synthase expression in aqueous humor or/and peripheral blood from fifty-six patients with retinitis pigmentosa and sixty subjects without systemic or ocular …
Model Eye Incorporating a Manually Tunable Polymer Lens and Microfluidics Chamber for Simulation of Vitreous Floaters
2016
a model eye incorporating a manually tunable lens and microfluidics chamber for simulation of vitreous floaters has been described. Aberrations correspond to average values in population and diffraction effects could be observed.
Ocular gelling microspheres: in vitro precorneal retention time and drug permeation through reconstituted corneal epithelium.
2008
Purpose: The model drug norfloxacin (NOR)was encapsulated into trehalose (TRH) and hydroxyethylcellulose(NAT) microspheres to obtain a novel gelling ophthalmic delivery system for prolonged release on corneal tissue. Methods: We assessed NOR release from microspheres, prepared by the emulsion-solvent evaporation method. A new in vitro tear turnover model, including inserts containing reconstituted human corneal epithelium (RHC), was designed to evaluate the TRH/NAT microspheres’ precorneal retention time. Bioadhesive properties of TRH/NAT microspheres were validated by using drug-loaded microspheres prepared with gelatine (GLT) commonly used as reference material in adhesion studies. Result…
Physicochemical Surface Properties of Various Intraocular Lenses
2001
<i>Purpose:</i> It is of major interest to understand how intraocular lens (IOL) surfaces interact with aqueous humor or tissues after implantation. Important parameters to describe the physicochemical surface properties are the contact angle and critical surface tension. Therefore, we performed measurements of the contact angle and critical surface tension of several commercially available IOLs. <i>Setting:</i> Department of Ophthalmology, Johannes Gutenberg University, Mainz, University Eye Hospital, Tübingen, and Max Planck Institute for Polymer Research, Mainz, Germany. <i>Methods:</i> A total of 17 commercially available standard quality IOL types (6…
Antibodies to alpha B-crystallin, vimentin, and heat shock protein 70 in aqueous humor of patients with normal tension glaucoma and IgG antibody patt…
2007
To show the existence of IgG antibodies against retinal antigens in aqueous humor of normal tension glaucoma patients.Forty-two patients were included in this study. Aqueous humor was collected from control subjects (CO; n = 21) and patients with normal tension glaucoma (NTG; n = 21). Western blot methods against bovine retinal antigens were used to detect the IgG antibody patterns. The complex antibody repertoires were analyzed by multivariate statistical techniques. Mass spectrometry was used to identify the most important antigens.Very complex IgG antibody patterns against retinal antigens were found in all analyzed aqueous humor samples. Our multivariate approach could quantify differen…
Penetration of 2% cyclosporin eyedrops into human aqueous humour.
1989
The penetration into the eye and the systemic absorption of 2% cyclosporin eye drops were determined by polarisation immunofluorescent assay of cyclosporin in the aqueous humour and plasma of 30 patients at the time of cataract surgery. The results were then compared with the corresponding results after oral administration at a dosage of 5 mg/kg/day to three further patients. The maximum intraocular concentration (24 (SD 9) mg/l) was achieved four hours after topical administration. This level was slightly less than that found in aqueous humour (28 (SD 10) mg/l) 12 hours after systemic administration of the drug at a dosage of 5 mg/kg/day. Both these levels are below the minimum therapeutic…
Das Verhalten des Trabeculum corneo-sclerale nach Implantation in die Augenvorderkammer bei Primaten
1962
Um das reaktive Verhalten des Trabeculum corneo-sclerale unter Bedingungen der Gewebekultur zu prufen, wurden bei Meerkatzen (Cercopithecus aethiops) Auto- und Heterotransplantationen von Kammerwinkelgewebsstucken (Elliot-Trepanate) in die Augenvorderkammer durchgefuhrt. Die lichtmikroskopische und histochemische Aufarbeitung des Materials zeigte, das das normalerweise hochdifferenzierte Trabekelwerk des Implantates vom 3. Tag post operationem zunehmend entdifferenziert. Zuerst gingen die spezialisierten Endothelien der Innenwand des Schlemmschen Kanals, spater die Trabekelmembranen und die intertrabekularen Stromungskanalchen verloren. Die lamellare Ordnung war nicht mehr erkennbar. Das Ge…
Proteome alterations in aqueous humour of primary open angle glaucoma patients.
2019
Aim To unravel the primary open angle glaucoma (POAG) related proteomic changes in aqueous humour (AH). Methods Totally 35 patients listed for cataract surgery (controls: n=12, age: 67.4±13.6y) or trabeculectomy for POAG (n=23, age: 72.5±8.3y) were included. AH samples of those patients were obtained during cataract surgery or trabeculectomy. AH samples were subsequently pooled into the experimental groups under equal contribution in terms of protein amount of each individual patient. Protein samples were analyzed by a linear trap quadrupol Orbitrap Mass Spectrometry device with an upstream liquid chromatography system. The obtained raw data were analyzed using the Maxquant proteome softwar…