Search results for "assembly"

showing 10 items of 768 documents

Direct squencing from the minimal number of DNA molecules needed to fill a 454 picotiterplate

2014

Notice of Republication: This article was republished on June 17, 2014, to correct an error in the title. The publisher apologizes for the error. In addition, a typographical error was corrected in the Abstract. Please download this article again to view the correct version. The originally published, uncorrected article and the republished, corrected article are provided here for reference.

ScienceSequence assemblyHybrid genome assemblyBiologyDNA sequencingDeep sequencingGens humans MapatgeSequencing by hybridizationMapatgeEscherichia coliGeneticsCluster AnalysisGenome SequencingMolecular Biology TechniquesSequencing TechniquesMolecular BiologyGene LibraryGeneticsWhole Genome AmplificationMultidisciplinaryGenètica bacterianaShotgun sequencingQRMultiple displacement amplificationChromosome MappingHigh-Throughput Nucleotide SequencingBiology and Life SciencesComputational BiologySequence Analysis DNAGenomicsGenome AnalysisGens humansMedicineSequence AnalysisGenome BacterialResearch Article
researchProduct

Functional roles of the membrane-associated AAV protein MAAP

2021

AbstractWith a limited coding capacity of 4.7 kb, adeno-associated virus (AAV) genome has evolved over-lapping genes to maximise the usage of its genome. An example is the recently found ORF in the cap gene, encoding membrane-associated accessory protein (MAAP), located in the same genomic region as the VP1/2 unique domain, but in a different reading frame. This 13 KDa protein, unique to the dependovirus genus, is not homologous to any known protein. Our studies confirm that MAAP translation initiates from the first CTG codon found in the VP1 ORF2. We have further observed MAAP localised in the plasma membrane, in the membranous structures in close proximity to the nucleus and to the nuclea…

SciencevirusesGenetic VectorsBiologyVirus ReplicationGenomeinfektiotArticleVirusViral Proteins03 medical and health scienceschemistry.chemical_compoundCapsidGene therapyPlasmidProtein sequencingHumansGeneparvovirukset030304 developmental biology0303 health sciencesMultidisciplinaryMolecular engineeringVirus Assembly030302 biochemistry & molecular biologyQVirionRMembrane ProteinsTranslation (biology)DependovirusCell biologyCapsidchemistryMedicineCapsid ProteinsproteiinitDNAPlasmidskapsidi
researchProduct

The use of sea waves for generation of electrical energy: a linear tubular asynchronous electrical generator

2007

A linear tubular asynchronous generator to be used as generator driven by seawave is presented. The design criteria of a TLIM with hollowed iron induced part as well as the technological processes to be adopted and the materials utilized in the various parts are described. The methodologies for mechanical assembling and electric wiring are considered too. Finally, the prototype with bimetallic induced part that has been designed and built is presented and some experimental results on electrical and mechanical variables are shown.

Sea wavesEngineeringbusiness.industryElectric potential energyInduction generatorMechanical engineeringElectric generatorInduction generators Stator windings Iron Prototypes Frequency Permanent magnets Voltage Telecommunications AssemblySettore ING-IND/32 - Convertitori Macchine E Azionamenti Elettricilaw.inventionGenerator (circuit theory)Asynchronous communicationlawWind waveWave power generationbusinessOCEANS 2007
researchProduct

Preface

2012

Self-Assembly Nanomaterials Molecular Machinery
researchProduct

Cholesterol Starvation and Hypoxia Activate the FVII Gene via the SREBP1-GILZ Pathway in Ovarian Cancer Cells to Produce Procoagulant Microvesicles

2019

AbstractInteraction between the transcription factors, hypoxia-inducible factor (HIF1α and HIF2α) and Sp1, mediates hypoxia-driven expression of FVII gene encoding coagulation factor VII (fVII) in ovarian clear cell carcinoma (CCC) cells. This mechanism is synergistically enhanced in response to serum starvation, a condition possibly associated with tumor hypoxia. This transcriptional response potentially results in venous thromboembolism, a common complication in cancer patients by producing procoagulant extracellular vesicles (EVs). However, which deficient serum factors are responsible for this characteristic transcriptional mechanism is unknown. Here, we report that cholesterol deficien…

Serum0301 basic medicineLeucine zipper030204 cardiovascular system & hematologyMice03 medical and health sciences0302 clinical medicineCell-Derived MicroparticlesCell Line Tumorhemic and lymphatic diseasesAnimalsHumansHypoxiaTranscription factorOvarian NeoplasmsTumor hypoxiaCoagulantsChemistryHematologyFactor VIIChromatin Assembly and DisassemblyHypoxia-Inducible Factor 1 alpha SubunitXenograft Model Antitumor AssaysMicrovesiclesChromatinCell biologySterol regulatory element-binding proteinCholesterol030104 developmental biologyFemaleSignal transductionSterol Regulatory Element Binding Protein 1Chromatin immunoprecipitationSignal TransductionTranscription FactorsThrombosis and Haemostasis
researchProduct

Nickel(II) 3,4;9,10-perylenediimide bis-phosphonate pentahydrate: A metal-organic ferromagnetic dye

2012

The new metal organic compound nickel(II) 3,4;9,10-perylenediimide bis-phosphonate pentahydrate, i.e. Ni-2[(PDI-BP)-(H2O)(2)]center dot 3H(2)O (1), has been synthesized and its structural and magnetic properties have been studied. Reaction of 3,4;9,10-perylenediimide bisphosphonate (PDI-BP, hereafter) ligand and nickel chloride in water resulted in the precipitation of a red and poorly crystalline solid (1). As the solid shows a poor crystalline organization of aggregates, the energy dispersive X-ray diffraction analysis (EDXD) technique has been used to obtain short-range order structural information of the single nanoaggregates by radial distribution function analysis. The overall structu…

Settore CHIM/03 - Chimica Generale e InorganicaPrecipitation (chemistry)LigandInorganic chemistryStackingchemistry.chemical_elementPhosphonateChlorideInorganic Chemistrychemistry.chemical_compoundCrystallographyNickelFerromagnetismchemistrymedicinePhysical and Theoretical ChemistryPerylenetem; self-assembly; hybrid materials; energy dispersive x-ray diffraction; ferromagnetic dye; layered materialsSettore CHIM/02 - Chimica Fisicamedicine.drug
researchProduct

Dicationic Organic Salts: our recent journey in their properties and applications

2014

Settore CHIM/06 - Chimica OrganicaDicationic organic salts ionic liquids gel phases self-assembly
researchProduct

Organic Salts as Tectons for Self-assembly Processes in Solution

2022

The literature, covering the last decade, about the self-assembly processes of organic salts in conventional solvents was analyzed. In particular, data reported about imidazolium and ammonium salts have been considered. The analysis shows that these processes are highly determined by structural features of the salts. Indeed, besides the nature of the cationic head, features of the alkyl chain borne on the cation structure and its possible functionalization, as well as the nature of the anion play a pivotal role. These factors determine not only the nature of the solvent in which the process occurs but also the nature of the self-assembly mechanism. Consequently, the structure tunability of …

Settore CHIM/06 - Chimica OrganicaSelf assembly supramolecular chemistry electrostatic interactions.
researchProduct

Self-assembly process of diimidazolium salts: the effect of the alkyl chain length and isomeric substitution

Settore CHIM/06 - Chimica Organicaself- assembly imidazolium salts
researchProduct

Palermo si cura. Pratiche culturali di mutuo soccorso come risposta alle fragilità della città (post)pandemica

2023

In un quadro di riferimento in cui la crisi è globalizzata ma diseguale e in mancanza di un sistema di welfare capace di rispondere alle urgenze dei contesti e dei gruppi sociali più marginalizzati, una risposta ai nuovi bisogni sociali ed individuali è stata offerta da reti solidali e da pratiche di mobilitazione dal basso che svolgono un ruolo di agency territoriale, spesso indipendentemente o in assenza di Istituzioni. Nel contesto palermitano un impegno agito da una pluralità di soggetti culturali già attivi sul territorio, riuniti in un’assemblea pubblica (Palermo si cura), durante il periodo del primo lockdown hanno dato vita ad azioni di mutuo soccorso per rispondere all’emergenza ec…

Settore ICAR/21 - UrbanisticaIn a framework in which the crisis is globalized but unequal and in the absence of a welfare system capable of responding to the urgencies of the most marginalized contexts and social groups a response to the new social and individual needs has been offered by networks of solidarity and from bottom-up mobilization practices that play a role of territorial agency often independently or in the absence of institutions. In the Palermo context a commitment made by a plurality of cultural subjects already active in the area gathered in a public assembly (Palermo si cura) during the period of the first lockdown gave rise to mutual aid actions to respond to the economic emergency and social and offer a proposal to cure the fragility of the city based not on charity but on the claim of a political dimension of collective action. Starting from the analysis of initiatives such as "Un sorriso per Ballarò" and "Abbiamo un (bi)sogno" the contribution analyzes the role the incidence and the fragility of these experiences in providing answers - often filling gaps - to a general interest and how these processes of value creation can hopefully contribute to the construction of local government policies capable of addressing emerging issues.
researchProduct