Search results for "assembly"
showing 10 items of 768 documents
PIL.20-21-Unit 7. The role of the United Nations
2020
Public International Law. Unit 7. The role of the United Nations. Powerpoint of the Academic course 2020-2021.
Sulfate-Templated 2D Anion-Layered Supramolecular Self-Assemblies
2019
Summary Using solution and solid-state analyses, we demonstrate that the tripodal N-methylated(1,3,5-benzene-tricarboxamide)-tris(phenylurea) BTA ligands, possessing urea functionalities in the meta position, are able to form extended self-assembly 2D networks via hydrogen bonding templated by sulfate (SO42–). The divergence of the urea binding sites confers a propeller-like conformation to the ligands and is key to formation of the self-assemblies. Studies in solution and in the solid state as well as scanning electron microscopy (SEM) on the self-assembly properties of the ligands showed that the convergence also leads to the formation of hierarchical structures, including porous films an…
Low-temperature molecular layer deposition using monifunctional aromatic precursors and ozone-based ring-opening reactions
2017
Molecular layer deposition (MLD) is an increasingly used deposition technique for producing thin coatings consisting of purely organic or hybrid inorganic-organic materials. When organic materials are prepared, low deposition temperatures are often required to avoid decomposition, thus causing problems with low vapor pressure precursors. Monofunctional compounds have higher vapor pressures than traditional bi- or trifunctional MLD precursors, but do not offer the required functional groups for continuing the MLD growth in subsequent deposition cycles. In this study, we have used high vapor pressure monofunctional aromatic precursors in combination with ozone-triggered ring-opening reactions…
Purified Membrane-Containing Procapsids of Bacteriophage PRD1 Package the Viral Genome
2009
Icosahedral-tailed double-stranded DNA (dsDNA) bacteriophages and herpesviruses translocate viral DNA into a preformed procapsid in an ATP-driven reaction by a packaging complex that operates at a portal vertex. A similar packaging system operates in the tailless dsDNA phage PRD1 (Tectiviridae family), except that there is an internal membrane vesicle in the procapsid. The unit-length linear dsDNA genome with covalently linked 5'-terminal proteins enters the procapsid through a unique vertex. Two small integral membrane proteins, P20 and P22, provide a conduit for DNA translocation. The packaging machinery also contains the packaging ATPase P9 and the packaging efficiency factor P6. Here we…
Fragmentation and Coverage Variation in Viral Metagenome Assemblies, and Their Effect in Diversity Calculations
2015
Metagenomic libraries consist of DNA fragments from diverse species, with varying genome size and abundance. High-throughput sequencing platforms produce large volumes of reads from these libraries, which may be assembled into contigs, ideally resembling the original larger genomic sequences. The uneven species distribution, along with the stochasticity in sample processing and sequencing bias, impacts the success of accurate sequence assembly. Several assemblers enable the processing of viral metagenomic data de novo, generally using overlap layout consensus or de Bruijn graph approaches for contig assembly. The success of viral genomic reconstruction in these datasets is limited by the de…
Replication of hepatitis C virus
2000
The evolution of collective infectious units in viruses
2019
Viruses frequently spread among cells or hosts in groups, with multiple viral genomes inside the same infectious unit. These collective infectious units can consist of multiple viral genomes inside the same virion, or multiple virions inside a larger structure such as a vesicle. Collective infectious units deliver multiple viral genomes to the same cell simultaneously, which can have important implications for viral pathogenesis, antiviral resistance, and social evolution. However, little is known about why some viruses transmit in collective infectious units, whereas others do not. We used a simple evolutionary approach to model the potential costs and benefits of transmitting in a collect…
Tīmekļa lietotņu veiktspējas optimizācija izmantojot WebAssembly
2019
Bakalaura darba “Tīmekļa lietotņu veiktspējas optimizācija izmantojot WebAssembly” mērķis ir noskaidrot WebAssembly izmantošanas un nepieciešamības apstākļus, kā arī veikt pētijumu par tīmekļa lietotnes veiktspējas analīzi pēc moduļu pārstrādes uz WebAssembly kompilētu kodu, lai varētu noteikt, kad to ir vērts izmantot. Darbs sika sadalīts divās sadaļās. Pirmajā sadaļā tika veikta analīze par WebAssembly, tā darbības principiem, kā arī izmantošanu programmatūres izstrādē. Otrajā sadaļā tiks aprakstīts izveidotais projekts, kā arī salīdzināts tīmekļa lietotnes kods un veiktspēja pirms un pēc moduļu pārstrādes. Bakalaura darbs izklāstīts 20 lappusēs, ilustrēts ar 15 attēliem un 4 tabulām. Lit…
WebAssembly iespēju izpēte, veicot klienta puses lietotnes salīdzinājumu .Net un JS vidēs
2018
Darbā tiek izpētīta tīmekļa programmēšanas vēsture un mūsdienas, aplūkojot dažādas tīmekļa lietotņu izstrādes iespējas. Darbā tiek izpētīti dažādi vienas lapas lietotņu izstrādes ietvari un paradigmas. Izmantojot izpētes rezultātus, darba praktiskajā daļā tika izstrādātas divas lietotnes, kas demonstrē vienādas iespējas izmantojot divas dažādas programmēšanas valodas – TypeScript un C#. Izstrādātajām lietotnēm tika aprakstīta un salīdzināta to izstrādes gaita, kā arī tika iegūti kvantitatīvi dati, kas apraksta ietvaru piemērotību tīmekļa lietotņu izstrādei.
De novogenome assembly of the land snailCandidula unifasciata(Mollusca: Gastropoda)
2021
AbstractAmong all molluscs, land snails are an economically and scientifically interesting group comprising edible species, alien species and agricultural pests. Yet, despite its high diversity, the number of whole genomes publicly available is still scarce. Here, we present the draft genome assembly of the land snailCandidula unifasciata, a widely distributed species along central Europe, which belongs to Geomitridae family, a group highly diversified in the Western-Palearctic region. We performed a whole genome sequencing, assembly and annotation of an adult specimen based on PacBio and Oxford Nanopore long read sequences as well as Illumina data. A genome of about 1.29 Gb was generated w…