Search results for "auto"

Translocation of the nuclear autoantigen La to the cell surface of herpes simplex virus type 1 infected cells.

1992

Recently we developed a procedure to translocalize one of the extractable nuclear antigens (ENAs), the La protein, to the cell surface of CV-1 cells. Here we report that herpes simplex virus type 1 infection can also induce a translocation of the autoantigen to the cell surface. On the cell surface we detected La protein assembled with large protrusions. Within these protrusions La protein colocalized with virus particles. These protrusions are known to be released from the cell after virus infections. Such complexes consisting of self and virus could provide helper determinants for an anti-self response, and therefore be important in generation of autoimmunity.

The human autoantigen La/SS-B accelerates herpes simplex virus type 1 replication in transfected mouse 3T3 cells.

1998

SUMMARY Permanently transfected mouse cell lines which expressed different levels of the human autoantigen La/SS-B were infected with different strains of herpes simplex virus type 1, including the strains ANG, HSZP, 17syn+ and HFEM. During infection the localization of the human La protein was followed using an anti-La MoAb, which recognized only the human La protein but did not cross-react with either the endogenous mouse La protein or any viral encoded protein. After infection La protein was transported from the nucleus to the cytoplasm. The time course of translocation was dependent on the amount of human La protein expressed in the respective cell line. Moreover, acceleration of viral …

Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme

1997

During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is hom…

Visual Contrast Modulates Operant Learning Responses in Larval Zebrafish.

2018

The larval zebrafish is a promising vertebrate model organism to study neural mechanisms underlying learning and memory due to its small brain and rich behavioral repertoire. Here, we report on a high-throughput operant conditioning system for zebrafish larvae, which can simultaneously train 12 fish to associate a visual conditioned pattern with electroshocks. We find that the learning responses can be enhanced by the visual contrast, not the spatial features of the conditioned patterns, highlighted by several behavioral metrics. By further characterizing the learning curves as well as memory extinction, we demonstrate that the percentage of learners and the memory length increase as the co…

Visual occlusion as tool to assess attentional demand and spare capacity

2021

Alla ricerca dell'autosostenibilità. Visioni e scenari per territorio e comunità

2021

Riflettere e ritornare a ripensare le relazioni tra le varie arti e i vari campi del sapere scientifico è oggi quanto mai necessario vista la tendenza esplicita alla liquefazione, allo scollamento e alla dispersione tra e dei processi intellettuali (sempre più omologati sulla necessità di aver successo mediatico nel messaggio immediato e mediante slogan e packaging più che risolvere, concretamente, i fatti urbani e territoriali) e alla costante separazione con le tecniche, i metodi e le attività burocratiche (anche esse sempre più meccaniche e industriali che fanno spesso il paio con processi di economie di scala). Entrambi gli aspetti divengono sempre più seriali, globalizzati e standardiz…

Impacts of Touch Screen Size, User Interface Design, and Subtask Boundaries on In-Car Task's Visual Demand and Driver Distraction

2020

Visual distraction by secondary in-car tasks is a major contributing factor in traffic incidents. In-car user interface design may mitigate these negative effects but to accomplish this, design factors’ visual distraction potential should be better understood. The effects of touch screen size, user interface design, and subtask boundaries on in-car task's visual demand and visual distraction potential were studied in two driving simulator experiments with 48 participants. Multilevel modeling was utilized to control the visual demands of driving and individual differences on in-car glance durations. The 2.5” larger touch screen slightly decreased the in-car glance durations and had a diminis…

Motifs de démotorisation des actifs et retraités dans les aires urbaines françaises : premiers résultats statistiques de l'enquête MoDe

2021

Based on a nationwide quantitative survey conducted on 2,000 households (including 1,000 demotorised households and 1,000 non-demotorised twin households), we propose to provide an overview of demotorisation in France. This survey by questionnaire was conducted during the winter of 2017-2018 with a panel of households representative of the French population (from the Metaskope panel of Kantar-TNS ex-TNS-Sofres). Combined with the ?Parc Auto? panel survey (Kantar-TNS for Ademe-CCFA-Ifsttar-DSR), this survey is an original source of data on the French demotorised households. From two multinomial logit regression models, for both active and retired populations, this data allows to highlight th…

MODELING OF VOLATILITY IN THE ROMANIAN CAPITAL MARKET

2012

This paper aims to analyze the volatility of capital market in Romania by selecting a portfolio of representative indices (BET BET_FI and RASDAQ_C). In this respect, we want to identify the most appropriate model to estimate volatility by using modern econometric tools and useful GARCH models respectively. The study results highlight that EGARCH(1,1) model has managed to eliminate all traces of statistically significant autocorrelation and ARCH effects from the residuals from daily series, giving an accurate image of the Romanian capital market volatility.

Vulnerabilità sulla scena. Temi etici e soluzioni drammaturgiche nella "Medea" di Seneca

2018

La Medea di Seneca racconta una storia di vulnerabilità? Questo saggio propone una lettura della tragedia usando il concetto di vulnerabilità non come criterio per verificare la correttezza degli assunti stoici in materia di condotta morale, ma quale paradigma di relazione tra soggetti che, per definirsi, si consegnano gli uni agli altri, esponendosi, e su tale esposizione mettono alla prova l’identità personale e la fallibilità dell’autonomia morale. In questa idea di vulnerabilità non si trova solo una lente per formulare domande di natura etica, ma anche un utile strumento di analisi del meccanismo drammaturgico senecano. Is Seneca’s Medea a story of vulnerability? This paper aims to rea…