Search results for "bacterial"
showing 10 items of 3246 documents
Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells
2022
Like many bacterial species, Borrelia burgdorferi, the pleomorphic bacterium that causes Lyme borreliosis, produces outer membrane vesicles (OMVs). Borrelial OMVs (BbOMVs) have been identified as containing virulence factors, such as outer surface proteins (Osps) A, B, and C, as well as DNA. However, the pathogenicity of BbOMVs in disease development is still unclear. In this study, we characterized purified BbOMVs by analyzing their size and immunolabeling for known antigenic markers: OspA, OspC, p39, and peptidoglycan. In addition, BbOMVs were cocultured with human non-immune cells for cytotoxicity analysis. The results demonstrated that, on average, the vesicles were small, ranging betwe…
Ekstracelulārās vezikulās ietvertās mikrobiālās RNS atklāšana cilvēku asins un urīna paraugos
2020
Gandrīz visi cilvēka šūnu tipi sekretē ekstracelulārās vezikulas (EVs), un tām ir svarīga loma starpšūnu komunikācijā. Jaunākie pētījumi liecina, ka komunikācija notiek arī starp cilvēka mikrobiotu un saimniekorganisma šūnām. Šajā pētījumā mēs parādām, ka no cilvēka asinīm un urīna izdalītās EVs satur RNS fragmentus no dažādiem mikroorganismiem. Visbiežāk sastopami baktēriju tipi un supertipi asinīs un urīnā bija Proteobacteria, PVC grupa un Terrabacteria. Lai noteiktu vai šo mikrobiālo transkriptu galvenais avots ir zarnu mikrobioms, mēs izstrādājām EV izdalīšanas metodi no cilvēku fēču paraugiem. Tālāk mēs plānojām veikt RNS sekvencēšanas (RNA-seq) analīzi EV, kas izdalītas no plazmas, ur…
Bacterial and chemical indicator of coastal water pollution.
1993
This study has been carried out to compare the presence of some organic compounds of long environmental persistance (OCLEP) and heavy metals, in sea water samples, with faecal pollution indicators. Along the Sicily coast line three sites with different grade of pollution (urban, agricultural, industrial) were sampled. 69 samples were obtained from October 1987 to May 1990. Only indicators of faecal pollution were found linked to human settlements, while the other investigated anthropogenic activity related substances were found independently from sampling area.
Characterization of bacterial community in indoor environment
2006
We report the presence of bacterial colonization in library environment and on the books housed, performing morphological and bacterial molecular analyses. Isolated bacterial colonies were characterised by 16S rRNA locus and ITS (16S-23S rRNA Intergenic Transcribed Spacer) amplification and sequencing. The results of SEM observation and molecular analysis showed the prevalent presence of bacteria belonging to the family Micrococcaceae. We distinguished Kocuria rosea, Arthrobacter nicotianae, Micrococcus luteus and Curtobacterium flaccumfaciens which shows high cellulolytic activity.
Bakteriofāgu terapijas iespējas
2016
Pārmērīga antibakteriālu līdzekļu lietošana veicina epidēmijas mēroga bakteriālās rezistences pieaugumu attīstītajās un attīstības valstīs. Bakteriālās rezistences pieaugums atdzīvina interesi par alternatīvām antibakteriālām terapijām. Viena no tām iesaista bakteriofāgus – vīrusus, kuriem piemīt spējas inficēt un vairoties baktēriju saimniekšūnās veicinot to lizēšanu. Ir plaši dokumentēta fāgu veikta vairāku rezistentu baktēriju izskaušana, tomēr nav skaidrs, caur kādiem mehānismiem tā tiek panākta. Tas apgrūtina regulējošo iestāžu atļauju izsniegšanu, lai ieviestu bakteriofāgu terapiju klīniskajā praksē. Bakalaura darba mērķis bija noskaidrot veselības aprūpes speciālistu (ārstu) viedokli…
Cooperation between Different CRISPR-Cas Types Enables Adaptation in an RNA-Targeting System
2021
CRISPR-Cas systems are immune systems that protect bacteria and archaea against their viruses, bacteriophages. Immunity is achieved through the acquisition of short DNA fragments from the viral invader’s genome.
Silent rain: does the atmosphere-mediated connectivity between microbiomes influence bacterial evolutionary rates?
2020
ABSTRACT Air carries a vast number of bacteria and viruses over great distances all the time. This leads to continuous introduction of foreign genetic material to local, established microbial communities. In this perspective, I ask whether this silent rain may have a slowing effect on the overall evolutionary rates in the microbial biosphere. Arguably, the greater the genetic divergence between gene ‘donors’ and ‘recipients’, the greater the chance that the gene product has a deleterious epistatic interaction with other gene products in its genetic environment. This is due to the long-term absence of check for mutual compatibility. As such, if an organism is extensively different from other…
Tempo and mode of early gene loss in endosymbiotic bacteria from insects
2006
Abstract Background Understanding evolutionary processes that drive genome reduction requires determining the tempo (rate) and the mode (size and types of deletions) of gene losses. In this study, we analysed five endosymbiotic genome sequences of the gamma-proteobacteria (three different Buchnera aphidicola strains, Wigglesworthia glossinidia, Blochmannia floridanus) to test if gene loss could be driven by the selective importance of genes. We used a parsimony method to reconstruct a minimal ancestral genome of insect endosymbionts and quantified gene loss along the branches of the phylogenetic tree. To evaluate the selective or functional importance of genes, we used a parameter that meas…
Comparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout
2021
This dataset consists of three phage therapy experiments to prevent Flavobacterium columnare infections in rainbow trout. Experiments I and II were done in stagnant water, and Experiment III in a flow-through system. See the documentation file attached with the dataset for full description of the methods (also found in the original article).
Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria
2013
2 figuras, 2 tablas