Search results for "bakteeri"

Honeybees affect floral microbiome composition in a central food source for wild pollinators in boreal ecosystems

2021

AbstractBasic knowledge on dispersal of microbes in pollinator networks is essential for plant, insect, and microbial ecology. Thorough understanding of the ecological consequences of honeybee farming on these complex plant–pollinator–microbe interactions is a prerequisite for sustainable honeybee keeping. Most research on plant–pollinator–microbe interactions have focused on temperate agricultural systems. Therefore, information on a wild plant that is a seasonal bottleneck for pollinators in cold climate such as Salix phylicifolia is of specific importance. We investigated how floral visitation by insects influences the community structure of bacteria and fungi in Salix phylicifolia inflo…

Fight evolution with evolution: plasmid‐dependent phages with a wide host range prevent the spread of antibiotic resistance

2013

The emergence of pathogenic bacteria resistant to multiple antibiotics is a serious worldwide public health concern. Whenever antibiotics are applied, the genes encoding for antibiotic resistance are selected for within bacterial populations. This has led to the prevalence of conjugative plasmids that carry resistance genes and can transfer themselves between diverse bacterial groups. In this study, we investigated whether it is feasible to attempt to prevent the spread of antibiotic resistances with a lytic bacteriophage, which can replicate in a wide range of gram‐negative bacteria harbouring conjugative drug resistance–conferring plasmids. The counter‐selection against the plasmid was sh…

Antibacterial Efficiency of Surface-Immobilized Flavobacterium-Infecting Bacteriophage

2019

Control of bacterial diseases by bacteriophages (phages) is gaining more interest due to increasing antibiotic resistance. This has led to technologies to attach phages on surfaces to form a biomaterial that can functionally display phages that interact with bacteria, to carry out successful infection cycles. Such a material could be applied in many environments, where the target pathogens are expected. Although this approach has been applied successfully in a few studies already, the basis of the antibacterial effect by the immobilized phages is unclear, and the interpretation of the results depends on the study. Here, we studied the phage attachment density, their detachment rate and infe…

Exploring phage-bacterium interactions : new ways to combat a fish pathogen

2014

Dataset for Mucin induces CRISPR-Cas defense in an opportunistic pathogen

2022

This dataset contains raw data points of (1) phage-bacterium co-evolution experiment (flavobacterium columnare colony counts, phage counts, bacterial growth characteristics and number of CRISPR-Cas spacers) and (2) from competition experiment between Flavobacterium columnare and Aeromonas sp. For detailed method description, see the attached Documentation file.

Responses of zooplankton to cane sugar additions to a small humic lake, Alinen Mustajärvi

2010

The response to projected increase in dissolved organic carbon (DOC) loading to boreal lakes is being studied in lake Alinen Mustajärvi, a small humic lake in southern Finland, where labile DOC concentration has been artificially increased during 2008 and 2009 by monthly additions of cane sugar. As part of this wider project, in this thesis the responses of the zooplankton community to cane sugar addition were studied. Zooplankton samples were collected monthly in the ice-free periods of 2007, 2008 and 2009 and the mean densities and mean carbon biomasses of rotifers and crustacean zooplankton (cladocerans and copepods) were calculated. Stable isotope analyses (δ13C and δ15N) were made for …

Plasmid Viability Depends on the Ecological Setting of Hosts within a Multiplasmid Community

2022

Plasmids are extrachromosomal genetic elements, some of which disperse horizontally between different strains and species of bacteria. They are a major factor in the dissemination of virulence factors and antibiotic resistance. Understanding the ecology of plasmids has a notable anthropocentric value, and therefore, the interactions between bacterial hosts and individual plasmids have been studied in detail. However, bacterial systems often carry multiple genetically distinct plasmids, but dynamics within these multiplasmid communities have remained unstudied. Here, we set to investigate the survival of 11 mobilizable or conjugative plasmids under five different conditions where the hosts h…

PRD1-viruksen vaikutus bakteerisoluihin, jotka sisältävät antibioottivastustuskyvyn antavan plasmidin

2010

Antibioottiresistenssi luokitellaan ihmisen terveyden suurimmaksi uhaksi. Antibioottiresistenssi lisääntyy bakteeripopulaatiossa konjugatiivisen plasmidin sisältävien bakteerien välityksellä jopa eri bakteerilajien välillä. PRD1-virus infektoi N-, P- ja W-tyypin konjugatiivisia plasmideja kantavia bakteereja. Tämän työn tarkoituksena oli selvittää, voisiko PRD1-viruksen avulla poistaa bakteeripopulaation vastustuskyky antibiooteille. Tätä testattiin kasvattamalla antibiooteille vastustuskykyisiä bakteereja ilman virusta ja viruksen läsnä ollessa. Bakteerien kasvukäyrät mitattiin lisäksi eri kasvuympäristöissä. Tulosten mukaan virus pudottaa populaation antibioottiresistenssin 100 %:sta jopa…

Suomalaisesta broilerintuotantoketjusta, broilerinlihasta ja koirista eristettyjen AmpC-positiivisten Escherichia coli -bakteerien resistenssiplasmid…

2014

Enterobacter cloacae administration induces hepatic damage and subcutaneous fat accumulation in high-fat diet fed mice.

2018

Accumulating evidence indicates that gut microbiota plays a significant role in obesity, insulin resistance and associated liver disorders. Family Enterobacteriaceae and especially Enterobacter cloacae strain B29 have been previously linked to obesity and hepatic damage. The underlying mechanisms, however, remain unclear. Therefore, we comprehensively examined the effects of E. cloacae subsp. cloacae (ATCC® 13047™) administration on host metabolism of mice fed with high-fat diet (HFD). C57BL/6N mice were randomly divided into HFD control, chow control, and E. cloacae treatment groups. The E. cloacae treatment group received live bacterial cells in PBS intragastrically twice a week, every ot…