Search results for "barcoding"

A molecular study of Neophyllaphis varicolor (Hemiptera, Aphididae) in Costa Rica

2019

The genus Neophyllaphis (Takahashi) (Aphididae: Neophyllaphidinae) is composed of 18 species; however, in the Americas only nine species have been reported previously. A new species, Neophyllaphis varicolor Miller & Halbert, was described in 2014 in USA. Colonies resembling those of this new species have been observed in Costa Rica on Podocarpus spp. In order to determine if N. varicolor is also present in Costa Rica, we sampled Neophyllaphis colonies from Podocarpus falcatus and P. chinensis. Additionally, we sampled individuals from Podocarpus sp. in Spain and Vietnam. DNA of each sample was extracted and used to amplify and sequence the cytochrome c oxidase subunit I (COI) and elongation…

A new Northeast Asian Lynceus (Crustacea: Branchiopoda: Laevicaudata) with uniquely modified thoracopods and an evaluation of DNA barcoding for clam …

2020

A new species of smooth clam shrimp (Branchiopoda: Laevicaudata) from Mongolia and China is described here based on both morphological and genetic differences. The new species, Lynceus grossipedia n. sp., has unique features, including asymmetrically modified male thoracopods (left side thoracopods III-VI), male claspers “movable finger” (=endopod) with delicate setation, and broad, bicarinate male and female rostrum. Lynceus grossipedia n. sp. is compared with the genera Paralimnetis Gurney, 1931 and Lynceiopsis Daday, 1912 and a recently described Lynceus Müller, 1776 from China, also showing modified male thoracopods. Lynceus mandsuricus Daday, 1927 is declared nomen inquirendum. DNA bar…

A contribution to the characterization of Ruppia drepanensis (Ruppiaceae), a key species of threatened Mediterranean wetlands

2021

To elucidate the taxonomic status of Ruppia drepanensis Tineo ex Guss. (Alismatales, Ruppiaceae), we performed morphological analysis and DNA barcoding of historical materials (including the lectotype) and fresh samples (including those from a recently discovered population near the locus classicus in Sicily, Italy). We conclude that R. drepanensis is a separate species, closely related to R. spiralis L. ex Dumort., that occurs in temporary inland waters from the western to central sectors of the Mediterranean region. We also highlight the importance of vouchers and the need to link molecular investigations to field, ecological, and morphological investigations.

DNA traces the origin of honey by identifying plants, bacteria and fungi

2021

The regional origin of a food product commonly affects its value. To this, DNA-based identification of tissue remains could offer fine resolution. For honey, this would allow the usage of not only pollen but all plant tissue, and also that of microbes in the product, for discerning the origin. Here we examined how plant, bacterial and fungal taxa identified by DNA metabarcoding and metagenomics differentiate between honey samples from three neighbouring countries. To establish how the taxonomic contents of honey reflect the country of origin, we used joint species distribution modelling. At the lowest taxonomic level by metabarcoding, with operational taxonomic units, the country of origin …

Metagenomic strategies to assess genetic diversity in Phytophthora species

Phylogenetic analysis of the Sicilian Hieracium taxa (Asteraceae) using "DNA barcoding": preliminary data

2014

DNA barcoding of the Mediterranean chondrichthyans (ELASMOMED)

2010

A Mediterranean record of Eulalia ornata (Annelida: Phyllodocidae) corroborating its fidelity link with the Sabellaria alveolata-reef habitat

2015

Among marine habitats Sabellaria alveolata -reefs deserve protection since they provide important ecosystem services and positive effects on biodiversity. Several marine species are listed among the S. alveolata -reef associated fauna, but characteristic species were seldom reported. Eulalia ornata (Annelida, Phyllodocidae) might represent an exception, since it appears common/abundant in S. alveolata -reefs of the Eastern Atlantic. The most evident geographical mismatch in the distributions of E. ornata and these biogenic reefs occurs in the Mediterranean Sea, where S. alveolata -reefs are commonly found, but E. ornata was never recorded, whilst E. viridis , a non-Mediterranean species, wa…

THE FINGERPRINT OF THE HUMAN GASTROINTESTINAL TRACT MICROBIOTA: A HYPOTHESIS OF MOLECULAR MAPPING

2017

The precise etiology of Inflammatory Bowel Disease (IDB) remains unclear and several factors are believed to play a role in its development and progression, including the composition of microbial communities resident in the gastrointestinal tract. Human intestinal microbiota are extensive with at least 15,000-36,000 bacterial species. However, thanks to the new development in sequencing and molecular taxonomic methodologies, our understanding of the microbiota population composition, dynamics, and ecology has greatly increased. Intestinal microbiota play a critical role in the maintenance of the host intestinal barrier homeostasis, while dysbiosis, which involves reduction in the microbiome…

ENHANCED BIOREMEDIATION E MONITORAGGIO MICROBIOLOGICO DI ACQUA CONTAMINATA DA SOLVENTI CLORURATI

2023

I solventi clorurati sono composti organici di sintesi appartenenti alla classe degli idrocarburi alifatici clorurati, largamente impiegati in diversi settori industriali e, come conseguenza, ampiamente diffusi nell’ambiente come inquinanti e particolarmente pericolosi anche per la salute umana. Tra i metodi di bonifica di siti contaminati da solventi clorurati, quelli basati sul biorisanamento sfruttano uno dei peculiari metabolismi batterici coinvolti nella biodegradazione di tali composti: la declorurazione riduttiva anaerobia in cui il composto clorurato è utilizzato come accettore terminale di elettroni in un vero e proprio processo di respirazione anaerobia, detta dealorespirazione; i…