Search results for "biodegradation"

Impact of electron beam irradiation on fish gelatin film properties

2016

International audience; The objective of this work was to display the effect of electron beam accelerator doses on properties of plasticized fish gelatin film. Electron spin resonance indicates free radical formation during irradiation, which might induce intermolecular cross-linking. Tensile strength for gelatin film significantly increases after irradiation (improved by 30% for 60 kGy). The vapour permeability is weakly affected by irradiation. Surface tension and its polar component increase significantly and are in accordance with the increase of wettability. So, irradiation may change the orientation of polar groups of gelatin at the film surface and crosslink the hydrophobic amino aci…

Involvement of an Alkane Hydroxylase System of Gordonia sp. Strain SoCg in Degradation of Solid n-Alkanes▿

2010

ABSTRACT Enzymes involved in oxidation of long-chain n -alkanes are still not well known, especially those in Gram-positive bacteria. This work describes the alkane degradation system of the n -alkane degrader actinobacterium Gordonia sp. strain SoCg, which is able to grow on n -alkanes from dodecane (C 12 ) to hexatriacontane (C 36 ) as the sole C source. SoCg harbors in its chromosome a single alk locus carrying six open reading frames (ORFs), which shows 78 to 79% identity with the alkane hydroxylase (AH)-encoding systems of other alkane-degrading actinobacteria. Quantitative reverse transcription-PCR showed that the genes encoding AlkB (alkane 1-monooxygenase), RubA3 (rubredoxin), RubA4…

Isolation of Gram-positive n-alkane degraders from a hydrocarbon-contaminated Mediterranean shoreline.

2007

Aims: To investigate the petroleum hydrocarbon (HC)-degrading potential of indigenous micro-organisms in a sandy Mediterranean coast, accidentally contaminated with petroleum-derived HCs. Methods and Results: Using culturable methods, a population of Gram-positive n-alkane degraders was detected in the contaminated soil. Five isolates, identified as one Nocardia, two Rhodococcus and two Gordonia strains, were able to degrade medium- and long-chain n-alkanes up to C36 as assessed by growth assays and gas chromatography-mass spectrometry analysis. Diverging alkane hydroxylase-encoding genes (alkB) were detected by PCR, using degenerated primers, in all the strains; multiple sequences were obt…

The growth of soil fungi Penicillium in the presence of N-(2-pyridylamino)methylenebisphosphonate as an alternative source of nutrients

2017

Kwas N-(2-pirydyloamino)metylenobisfosfonowy wykazują dużą aktywność herbicydową. W związku z tym należy zbadać jego odziaływanie na mikroorganizmy glebowe. Celem przeprowadzonych badań była ocena zdolności glebowych szczepów Penicillium do wzrostu w obecności kwasu N-(2-pirydyloamino)metylenobisfosfonowego jako alternatywnego źródła fosforu lub azotu lub węgla. Hodowle grzybów prowadzono w pełnym i zmodyfikowanym mineralnym podłożu Czapek, w temperaturze 25°C, przez 1–4 tygodnie. Modyfikacja podłoża polegała na wprowadzeniu 1 mM kwasu N-(2 pirydyloamino)metylenobisfosfonowego jako alternatywnego źródła składników odżywczych. Kontrolę względną stanowił wzrost grzybów w pełnym mineralnym pod…

Nitrous oxide emissions in a membrane bioreactor treating saline wastewater contaminated by hydrocarbons

2016

The joint effect of wastewater salinity and hydrocarbons on nitrous oxide emission was investigated. The membrane bioreactor pilot plant was operated with two phases: i. biomass acclimation by increasing salinity from 10 gNaCl L−1 to 20 gNaCl L−1 (Phase I); ii. hydrocarbons dosing at 20 mg L−1 with a constant salt concentration of 20 gNaCl L−1 (Phase II). The Phase I revealed a relationship between nitrous oxide emissions and salinity. During the end of the Phase I, the activity of nitrifiers started to recover, indicating a partial acclimatization. During the Phase II, the hydrocarbon shock induced a temporary inhibition of the biomass with the suppression of nitrous oxide emissions. The r…

A Biodegradable, Bio-Based Polymer for the Production of Tools for Aquaculture: Processing, Properties and Biodegradation in Sea Water

2023

Bio-based, biodegradable polymers can dramatically reduce the carbon dioxide released into the environment by substituting fossil-derived polymers in some applications. In this work, prototypes of trays for aquaculture applications were produced via injection molding by using a biodegradable polymer, Mater-Bi®. A characterization carried out via calorimetric, rheological and mechanical tests revealed that the polymer employed shows properties suitable for the production of tools to be used in aquaculture applications. Moreover, the samples were subjected to a biodegradation test in conditions that simulate the marine environment. The as-treated samples were characterized from gravimetrical,…

Wytwarzanie penicyliny G przez Penicillium chrysogenum jako marker biodegradacji związków fosfonoorganicznych

2011

Molecular approaches to study microbial mechanisms involved in pesticide biodegradation: atrazine as a case study

2006

Isolation and characterization of Gordonia SoCg n-alkane degradation cluster

2008

Gordonia strain SoCg is a Gram-positive GC rich n-alkane degrader, isolated from a long-term contaminated beach in Sicily for her ability to degrade long (dodecane C12) and very long chain n-alkanes up to hexatriacontane (C36) (P.Quatrini et al., J. Appl. Microbiol., 2007). PCR analysis, using degenerated primers, reveled that it carries one alkane-hydroxylase gene (alkB); PFGE and Southern analysis showed that this gene is localized on the chromosome. In order to isolate the alk cluster of Gordonia strain SoCg, an enriched gene bank was constructed in E.coli DH10B by isolating restriction fragments of the desired size from a preparative gel. One clone, containing a DNA insert of about 7 kb…

Comparative Investigation on the Soil Burial Degradation Behaviour of Polymer Films for Agriculture before and after Photo-Oxidation

2020

Polymer films based on biodegradable polymers, polyethylene (PE) and modified PE with oxo-degradable additive were prepared by film blowing. Carbon black (1%) was added to all the films. Commercial biodegradable Ecovio&reg