Search results for "bioluminescence"
showing 6 items of 46 documents
Semiquantitative bioluminescent assay of glutathione
1998
A novel technique has been developed for semiquantitative detection of glutathione (GSH) in small volumes of liquid samples. GSH is detected via enzymatic linkage to the NADP/NADPH + H+ redox system through glutathione reductase. Accumulated NADPH is measured via the bioluminescent FMN oxidoreductase bacterial luciferase reaction. A linear correlation is obtained between bioluminescence intensity of the luciferase reaction and the GSH content of the liquid sample. Possible applications of this procedure are discussed. © 1998 John Wiley & Sons, Ltd.
Dendrimēri kā luciferāzi saturošas plazmīdas transfekcijas vektori, in vitro un in vivo pētījumi
2018
Gēnu terapija ir nozīmīga dažādu ģenētisku slimību ārstēšanai, kā arī DNS vakcīnu attīstībai, taču vēljoprojām limitējošais faktors gēnu terapijas pielietošanai klīnikā ir drošas un efektīvas metodes trūkums, lai DNS ievadītu šūnās. Ir izveidoti dažādi sintētiski vektori, kas ir drošāki par rekombinanto vīrusu metodi, taču tie nav pietiekami efektīvi. Dendrimēri ir viens no sintētiskajiem DNS ievades vektoru veidiem, kam piemīt vairākas priekšrocības salīdzinot ar citām metodēm, un šīs nanodaļiņas pēdējo gadu laikā pastiprināti pētītas gēnu ievadei in vitro un in vivo. Šajā pētījumā tika pārbaudīta četru dažādu dendrimēru – ceturtās paaudzes poli(amidoamīna) PAMAM G4, ceturtās paaudzes katj…
The Silicon Photomultiplier: a promising photodetector for biosensing applications
Validation of ATP bioluminescence as a tool to assess antimicrobial effects of mouthrinses in an in vitro subgingival-biofilm model
2012
Objectives. The aim of this investigation was to evaluate whether the adenosine triphosphate (ATP) bioluminescence method is an appropriate tool to assess the efficacy of antiseptic mouthrinses in terms of quantitative reductions of total viable microbial counts in mixed biofilm populations in vitro. Study Design. Three mouthrinses, containing respectively, chlorhexidine and cetylpyridinium chloride (CHX/CPC), essential oils (EO) and amine fluoride/stannous fluoride (AFSF), as well as Phosphate Buffered Saline (PBS) used as control, were tested in an in vitro static biofilm model by ATP bioluminescence and compared to culture method. Biofilms were grown on saliva-coated hydroxyapatite disks…
Adenosine triphosphate bioluminescence in intensive care units: Be careful with its use
2016
LETTER TO EDITOR
Antibacterial Activity of Positively and Negatively Charged Hematite (α-Fe2O3) Nanoparticles to Escherichia coli, Staphylococcus aureus and Vibrio fi…
2021
This research and work has been supported by the European Regional Development Fund within the Activity 1.1.1.2 “Post-doctoral Research Aid” of the Specific Aid Objective 1.1.1 (i.e., “to increase the research and innovative capacity of scientific institutions of Latvia and the ability to attract external financing, investing in human resources and infrastructure”) of the Operational Programme “Growth and Employment” (No. 1.1.1.2/VIAA/2/18/331).