Search results for "bp"
showing 10 items of 672 documents
FKBP51 Affects TNF-Related Apoptosis Inducing Ligand Response in Melanoma
2021
Melanoma is one of the most immunogenic tumors and has the highest potential to elicit specific adaptive antitumor immune responses. Immune cells induce apoptosis of cancer cells either by soluble factors or by triggering cell-death pathways. Melanoma cells exploit multiple mechanisms to escape immune system tumoricidal control. FKBP51 is a relevant pro-oncogenic factor of melanoma cells supporting NF-κB-mediated resistance and cancer stemness/invasion epigenetic programs. Herein, we show that FKBP51-silencing increases TNF-related apoptosis-inducing ligand (TRAIL)-R2 (DR5) expression and sensitizes melanoma cells to TRAIL-induced apoptosis. Consistent with the general increase in histone d…
Highlighting curcumin-induced crosstalk between autophagy and apoptosis: A biochemical approach coupling impedancemetry, imaging, and flow cytometry
2019
Curcumin, a major active component of turmeric (Curcuma longa, L.), is known to have various effects on both healthy and cancerous tissues. In vitro studies suggest that curcumin inhibits cancer cell growth by activating apoptosis, but the mechanism underlying the anticancer effects of curcumin is still unclear. Since there is a consensus about endoplasmic reticulum (ER) stress being involved in the cytotoxicity of many natural compounds, we investigated by Amnis ® Imaging flow cytome-try the mechanistic aspects of curcumin's destabilization of the ER, but also the status of the lysosomal compartment involved in curcumin-associated apoptosis. Curcumin induces ER stress thereby causing an un…
Astringency and the interactions between a human salivary proline-rich protein and tannins
2015
International audience
The Role of Low Complexity Regions in Protein Interaction Modes: An Illustration in Huntingtin
2021
Low complexity regions (LCRs) are very frequent in protein sequences, generally having a lower propensity to form structured domains and tending to be much less evolutionarily conserved than globular domains. Their higher abundance in eukaryotes and in species with more cellular types agrees with a growing number of reports on their function in protein interactions regulated by post-translational modifications. LCRs facilitate the increase of regulatory and network complexity required with the emergence of organisms with more complex tissue distribution and development. Although the low conservation and structural flexibility of LCRs complicate their study, evolutionary studies of proteins …
A TR-FRET based functional assay for screening activators of CARM1
2013
Epigenome is an emerging field that demands selective cell-permeable chemical probes to perturb, especially in vivo, the activity of specific enzymes involved in modulating the epigenetic codes. Coactivator Associated Arginine (R) Methyltransferase 1 (CARM1) is a coactivator of estrogen receptor α (ERα), the main target in human breast cancer. We previously showed that overexpression of CARM1 by two-fold in MCF7 breast cancer cells increased the expression of ERα-target genes involved in differentiation and reduced cell proliferation, leading to the hypothesis that activating CARM1 by chemical activators may be therapeutically effective in breast cancer. Selective, potent, cell-permeable CA…
Psychometric Evaluation of the Basic Psychological Need Satisfaction and Frustration Scale (BPNSFS) in Italy
2017
The purpose of this multistudy report was to adapt the Basic Psychological Need Satisfaction and Frustration Scale (BPNSFS) to the Italian context. Two studies were conducted. In Study 1, we investigated the dimensionality, reliability, and convergent and discriminant validity of the instrument in a sample of 544 participants (males = 41%) from 16 to 35 years old. In Study 2, we replicated the results concerning dimensionality in an independent sample of 502 participants (males = 42%) from 16 to 35 years old. Furthermore, we analyzed measurement invariance across gender. Results of both studies showed that comparing a series of competitive factorial models, the 6-factor model had the best f…
Evidence Based Prevention: un nuovo modello in Sanità Pubblica
2005
The concept of evidence-based prevention (EBP), was developed during the last decade as the counterpart, in Public Health, of Evidence-Based Medicine (EBM) in Clinical Medicine. The growing debate on EBP reflects the increasing recognition that successful evaluation of public health interventions will necessarily entail the use of research designs other than controlled trials as well as the use of various other types of evidence, often in combination. Evidence-based prevention, therefore, aims to implement those prevention strategies that have been found to be useful and effective through a formal evaluation based on scientific methodology. A the same it aims to progressively discard all pr…
Analysis of Cardiac Pulse Arrival Time Series at Rest and during Physiological Stress
2022
The study of cardiovascular dynamics is pivotal in the prevention and monitoring of cardiovascular diseases. Pulse Arrival Time (PAT) series contain information concerning not only the dynamics of the Autonomic Nervous System (ANS), but of all the systems involved in the regulation of cardiovascular homeostasis. This study aims to highlight how indexes extracted from PAT series in time-, frequency- and information-domain allow to discriminate among different physiological conditions. Analyses were carried out on 76 young healthy subjects, at rest and during orthostatic or mental stress. Our results show that PAT indexes vary according to the ANS condition, and may thus be useful parameters …