Search results for "bp"
showing 10 items of 672 documents
Inhibition of VEGF expression through blockade of Hif1a and STAT3 signalling mediates the anti-angiogenic effect of melatonin in HepG2 liver cancer c…
2013
Background: Hepatocellular carcinoma (HCC) growth relies on angiogenesis via vascular endothelial growth factor (VEGF) release. Hypoxia within tumour environment leads to intracellular stabilisation of hypoxia inducible factor 1 alpha (Hif1α) and signal transducer and activator of transcription (STAT3). Melatonin induces apoptosis in HCC, and shows anti-angiogenic features in several tumours. In this study, we used human HepG2 liver cancer cells as an in vitro model to investigate the anti-angiogenic effects of melatonin. Methods: HepG2 cells were treated with melatonin under normoxic or CoCl2-induced hypoxia. Gene expression was analysed by RT–qPCR and western blot. Melatonin-induced anti-…
Darba procesa izveide platformā Microsoft SharePoint
2015
Microsoft SharePoint ir saistīts ar lieliem dokumentu apjomiem un dažādu mijiedarbību ar tiem. Lai padarītu darbu ar datiem vieglāku, SharePoint ļauj lietotajiem izveidot darbplūsmas SharePoint Designer programmatūrā. Tomēr, lietotājs var velēties izveidot darbplūsmu, kura nolasa vērtības no PDF formas laukiem. SharePoint Designer ne piedāvā darbplūsmas darbības kuras ir spējīgas to izdarīt. Tomēr, SharePoint dod iespēju lietotajiem pašiem izveidot vajadzīgas darbības un izmantot tas SharePoint dizaineri Kvalifikācijas darbs apraksta “smilškastes” (izolētā) risinājumā izveidi, kas pievieno papildus darbplūsmas darbības SharePoint dizainerī. Projekta mērķis ir izveidot darbplūsmas darbības k…
Extraction of Airways from CT (EXACT'09)
2012
Contains fulltext : 107854.pdf (Publisher’s version ) (Open Access) This paper describes a framework for establishing a reference airway tree segmentation, which was used to quantitatively evaluate fifteen different airway tree extraction algorithms in a standardized manner. Because of the sheer difficulty involved in manually constructing a complete reference standard from scratch, we propose to construct the reference using results from all algorithms that are to be evaluated. We start by subdividing each segmented airway tree into its individual branch segments. Each branch segment is then visually scored by trained observers to determine whether or not it is a correctly segmented part o…
„Stowarzyszenie Wychodźców Polskich pod opieką Królowej Korony Polskiej w diecezji częstochowskiej” w parafiach powiatu wieluńskiego
2020
Głównym problemem, z jakim musiało się zmierzyć społeczeństwo powiatu wieluńskiego po odzyskaniu przez Polskę niepodległości, była trudna sytuacja materialna, będąca skutkiem wysokiego poziomu bezrobocia. Wpłynęło to na ogromne zwiększenie wychodźstwa sezonowego do krajów Europy Zachodniej w celach zarobkowych. Ludność robotnicza traciła tam kontakt z wiarą katolicką. W trosce o życie religijne emigrujących diecezjan bp Teodor Kubina zorganizował „Stowarzyszenie Wychodźców Polskich pod opieką Królowej Korony Polskiej w diecezji częstochowskiej”, którego celem było zjednoczenie robotników sezonowych, podniesienie wśród nich poziomu moralnego i ugruntowanie postaw patriotycznych. Szczególną g…
Enseñanza de las ciencias : revista de investigación y experiencias didácticas
2021
Se expone el proceso seguido por un equipo de docentes de secundaria con el objetivo de cambiar la metodología de trabajo en el aula mediante el diseño y aplicación de una secuencia de aprendizaje definida a partir del aprendizaje basado en retos que integre de manera interdisciplinar los contenidos de Biología y Geografía en segundo de la ESO. Mediante la reflexión didáctica compartida, organizada y continuada se transforma una salida de campo tradicional en un nuevo proyecto de trabajo que se desarrolla a lo largo de un trimestre completo. Los resultados muestran la nueva secuencia de actividades creada, la percepción de los estudiantes y familias y los beneficios en la implicación del al…
Developmental toxicity of Bisfenol A (BPA) in Ciona intestinalis (Ascidiacea, Urochordata)
2009
H1° mRNA-containing complexes in rat brain cells. In: Proceedings of the Abstracts
2015
Post-transcriptional regulation of gene expression depends on RNA-binding proteins (RBPs), which are able to regulate translation, stability and subcellular localization of mRNAs [1]. RNA-protein complexes start to be built up since transcription; some proteins remain then bound to the transcript, while others behave as only transient components. In the developing nervous system of mammals, the postnatal production of the histone variants H1° and H3.3 is mainly regulated at the post-transcriptional level. Synthesis and incorporation into chromatin of the two histone proteins has been suggested to be involved in the epigenetic regulation of gene expression, both in normal brain development a…
Melanoma cells release extracellular vesicle which contain H1° linker histone as well as RNA-binding proteins which bind to the H1° mRNA
2015
We previously demonstrated that G26/24 oligodendroglioma cells release EVs that contain proteins, such as FasL and TRAIL, which induce apoptosis in rat cortical neurons [1] and astrocytes [2]. We also reported that cancer cells use EVs for transferring, into the environment [3], proteins such as extracellular matrix remodelling proteases [4], and H1°, a differentiation-specific histone [5]. In particular, by releasing H1°, cells could escape differentiation cues [5]. To verify the role of EVs in releasing specific proteins and mRNAs, in this study we used as a model A375 melanoma cells. METHODS EVs were purified from cell culture media as previously reported [1, 2]. T1 RNase-protection assa…
Expression and intracellular localization of H1° mRNA-containing complexes in developing rat brain and astrocytes
2015
INTRODUCTION: Post-transcriptional regulation of gene expression relies on RNA-binding proteins (RBPs), which regulate intracellular transport, stability, and translation of mRNAs [1]. We previously identified a set of proteins which interact with mRNAs encoding H1° and H3.3 histones [2-5]. All these proteins are probably part of a ribonucleoprotein particle [6]. Here we report more details on the expression and intracellular localization of some of these RBPs, during rat brain development and in isolated rat astrocytes. METHODS: Affinity chromatography was performed as already described [6]. Preparation of total lysates and cellular sub-fractions was done as reported in [3]. Possible co-lo…