Search results for "buffer"
showing 10 items of 230 documents
Deconvolution of the spectral line profiles for the plasma temperature estimation
2010
Abstract The Hg 253.7 nm spectral line profiles, emitted from the mercury–argon high-frequency electrodeless discharge lamps (HFEDL) have been measured by means of a high-resolution scanning Fabry–Perrot interferometer at the mercury cold spot temperature value at 20 °C, different discharge current and buffer gas values. The deconvolution procedure by means of the Tikhonov's regularization method was performed to obtain the real spectral line shape. The influence of the instrumental function and absorption, real width of the Hg 253.7 nm resonance line and temperature of the radiating atoms are obtained. The results were compared with the results of the nonlinear multiparameter mathematical …
Cooling of radioactive ions with the Penning trap REXTRAP
2005
Abstract Cooling of radioactive ion beams in a Penning trap is an essential component of the post-accelerator REX-ISOLDE at CERN. Prior to their charge-breeding and acceleration, ions from the on-line mass separator ISOLDE are accumulated, cooled and bunched with REXTRAP. This beam preparation provides short ion pulses with low emittance, key ingredient for a high efficiency of REX-ISOLDE. Two different cooling techniques have been investigated with REXTRAP. Both rely on the use of a buffer gas as the coolant but differ in the way the transversal compression of the stored ion cloud is achieved. Sideband cooling with a light buffer gas as coolant is the standard technique used at REXTRAP so …
Ion storage technique for very long living states: The decay rate of the 5D 3/2 state of Ba II
1979
The metastable 5D 3/2 state of Ba+ is used to demonstrate the possibility of the ion storage technique for the measurement of extremely long lived states. The ions are confined in a r.f. quadrupole trap and excited by a strong pulsed tunable dye laser to the 6P 1/2 state which partially decays into the 5D 3/2 state. Sampling of the time development of the ground state population leads to the determination of the 5D 3/2 decay rate. Using He as a buffer gas at a pressure of about 10−6 mbar to initially store the ions, varying the gas density and extrapolating to zero pressure, we find for the lifetime $$\tau _{D_{3/2} } = 17.5 \pm 4s$$ .
High-precision hyperfine spectroscopy inM1-M1 double-resonance transitions on trappedPb+207
1992
Optical pumping of the ground state of $^{207}\mathrm{Pb}^{+}$ ions, confined in a Paul ion trap on the weak 6${\mathit{P}}_{1/2\mathrm{\ensuremath{-}}}$6${\mathit{P}}_{3/2}$ M1 transition at \ensuremath{\lambda}=710 nm, and consequent magnetic-dipole transitions between the ground-state hyperfine Zeeman levels have resulted in an A factor of 12 968 180 601.61(0.22) Hz and linewidth of 0.5 Hz. The value includes corrections of Zeeman shifts from residual magnetic fields and second-order Doppler shifts in the several-eV-deep trap potential, while the ions were cooled by He buffer gas. Two M1 quanta have been applied simultaneously in a double-resonance experiment.
Experimental lifetime of the metastable 5D 3/2 state in Ba+
1992
A discrepancy exists between theoretical and experimental lifetimes of the metastable 5D3/2 state in Ba+. In order to redertermine that lifetime, we probe the population of the metastable 5D3/2 state of a Ba+ ion cloud stored in a Paul ion trap in the presence of He buffer gas as the function of time delay after pulsed laser excitation of this state. The measured decay rates at different buffer gas pressures are extrapolated to zero pressure and we obtain a radiative decay time of 48.0±5.9 s. This is not in agreement with theoretical predictions of about 80 s, but reduces the discrepancy from a previously reportet experimental value of 17.5 s. If the possibility of finestructure mixing to a…
Fourier-transform spectroscopy and description of low-lying energy levels in the B(1)(1)Π state of RbCs.
2013
The Fourier transform spectrometer with resolution of 0.03 cm(-1) was applied to disperse the diode laser induced B(1)(1)Π → X(1)Σ(+) fluorescence spectra of the RbCs molecule in a heat pipe. The presence of buffer gas (Ar) produced in the spectra the dense pattern of collision-induced rotation relaxation lines, thus enlarging the B(1)(1)Π data set, allowing to determine the Λ-splitting constants and to reveal numerous local perturbations. In total, 2664 term values for (85)Rb(133)Cs and (87)Rb(133)Cs in the energy range from 13,770 to 15,200 cm(-1) were obtained with accuracy about 0.01 cm(-1). A pointwise potential energy curve (PEC) based on inverted perturbation approach was constructed…
B1(1)Π state of KCs: high-resolution spectroscopy and description of low-lying energy levels.
2012
The diode laser induced B(1)(1)Π → X(1)Σ(+) fluorescence spectra of the KCs molecule were recorded by Fourier-transform spectrometer with resolution of 0.03 cm(-1). Buffer gas Ar was used to facilitate appearance of rotation relaxation lines in the spectra, thus enlarging the B(1)(1)Π dataset, allowing one to determine the Λ-splitting constants and to reveal numerous local perturbations. A dataset was systematically obtained for B(1)(1)Π vibrational levels ν(') ∈ [0; 5] nonuniformly covering rotational levels with J(') ∈ [7; 233]. For ν(') ∈ [0; 3], the less-perturbed data of f-components were included in the fit. A pointwise potential energy curve (PEC) based on the inverted perturbation a…
Potential for microbial diuron mineralisation in a small wine-growing watershed: from treated plots to lotic receiver hydrosystem
2009
BACKGROUND: Since biological degradation processes are known to be a major driver of the natural attenuation of pesticide residues in the environment, microbial communities adapted to pesticide biodegradation are likely to play a key environmental role in reducing pesticide exposure in contaminated ecosystems. The aim of this study was to assess the diuron-mineralising potential of microbial communities at a small-scale watershed level, including a diuron-treated vineyard (pollution source), its associated grass buffer strip (as a river protection area against pesticide runoff) and the lotic receiver hydrosystem (sediments and epilithon), by using radiorespirometry. RESULTS: Comparison of r…
Crosslinked α,β-Polyasparthydrazide Micromatrices for Controlled Release of Anticancer Drugs
1995
The preparation of new hydrogels by the reaction of α,β- polyasparthydrazide and glutaraldehyde is reported. A different crosslinking degree was obtained by varying the ratio crosslinking agent/polymer which influenced the swelling behavior of the gel. 5-Fluorouracil, was incorporated into the matrices during the crosslinking reaction and in vitro release studies were performed in simulated gastric juice (pH 1.1) and pH 7.4 buffer solution. The hydrogels prepared were chemically stable in the dissolution media. The observed data show the potential application of these new matrices for peroral administration of anticancer agents.
Fitness Trade-Offs Determine the Role of the Molecular Chaperonin GroEL in Buffering Mutations
2015
Molecular chaperones fold many proteins and their mutated versions in a cell and can sometimes buffer the phenotypic effect of mutations that affect protein folding. Unanswered questions about this buffering include the nature of its mechanism, its influence on the genetic variation of a population, the fitness trade-offs constraining this mechanism, and its role in expediting evolution. Answering these questions is fundamental to understand the contribution of buffering to increase genetic variation and ecological diversification. Here, we performed experimental evolution, genome resequencing, and computational analyses to determine the trade-offs and evolutionary trajectories of Escherich…