Search results for "caffold"

Biocompatibility evaluation of PLLA scaffolds for vascular tissue engineering

2015

Poly-L-lactic acid (PLLA), a hemicrystalline material, has been extensively studied in applications of engineered tissues, because it is biodegradable, absorbable and it supports cell attachment and growth. The purpose of this study is to evaluate tissue/ material interactions, neovascularization and the biocompatibility of PLLA by optical and scanning electron microscopy in a model of animal implant. PLLA porous disks were implanted into the dorsal subcutis of BALB/C mice for 1, 2, 3, and 8 weeks. The bioptic samples of excised PLLA and the surrounding tissue were evaluated for inflammatory response and tissue ingrowth. The samples were divided in two halves: one was fixed in neutral buffe…

Polyaspartamide-polylactide electrospun scaffolds for potential topical release of ibuprofen

2012

BIOGLASS® pre-treatment improves its integration in polymeric scaffolds

Tailoring PLLA scaffolds for tissue engineering applications: Morphologies for 2D and 3D cell cultures

2009

PLLA scaffold suitable for dermis regeneration were realized by Thermally Induced Phase Separation (TIPS) starting from a ternary solution PLLA/dioxane/water. The reconstruction of a complex tissues as the dermis implies the use of different cellular types (coculture), with different growth behaviour (2D vs. 3D). The scaffolds present an homogeneous porous surface to allow the keratinocytes 2D growth and a porous internal structure for the fibroblasts 3D growth. Our results show that the porosity of the surface can be tuned by changing the chemical nature of the sample holder (aluminium, teflon, polypropylene). A large variety of morphologies, in terms of average pore size and interconnecti…

A facile and eco-friendly route to fabricate poly(Lactic acid) scaffolds with graded pore size

2016

Over the recent years, functionally graded scaffolds (FGS) gaineda crucial role for manufacturing of devices for tissue engineering. The importance of this new field of biomaterials research is due to the necessity to develop implants capable of mimicking the complex functionality of the various tissues, including a continuous change from one structure or composition to another. In this latter context, one topic of main interest concerns the design of appropriate scaffolds for bone-cartilage interface tissue. In this study, three-layered scaffolds with graded pore size were achieved by melt mixing poly(lactic acid) (PLA), sodium chloride (NaCl) and polyethylene glycol (PEG). Pore size distr…

Characterization of PLLA scaffolds for biomedical applications

2017

The porosity and pore size distribution of three-dimensional scaffolds have direct implications on their biomedical applications (tissue engineering, drug delivery, and wound dressing). Accordingly, in this paper, a fast, facile, and conservative method relying on low-field nuclear magnetic resonance (LF-NMR) for the evaluation of mean pore size and pore size distribution of polymeric scaffolds is reported. The applicability of the technique is demonstrated on poly-L-lactic acid scaffolds fabricated using the thermal induced phase separation. Results obtained through LF-NMR are successfully compared to scanning electron microscope and X-ray microcomputed tomography micrographs.

Influence of scaffold pore size on collagen I development: A new in vitro evaluation perspective

2013

Bone tissue engineering takes part in the complex process of bone healing by combining cells, chemical/physical signals, and scaffolds with the scaffolds providing an artificial extracellular matrix network. The role of the support template for cell activity is crucial to guide the healing process. This in vitro study compared three different poly(D,L-lactic acid) scaffolds obtained by varying the pore size generated by applying different salt leaching processes. The influence of pore dimensions on the extracellular matrix produced by human osteosarcoma-derived osteoblasts (MG63 cell line) seeded on these different materials was analyzed. This study is targeted on the intermediate stage of…

Poly(ethylenglycol) mimics adhesive capability of the ECM treatment on 3D polylactide-based scaffolds to study in vitro human hepatocarcinoma process…

2011

Structural and functional evidence for the scaffolding effect of alveolar blood vessels

2017

A contribution of pulmonary blood distension to alveolar opening was first proposed more than 100 years ago. To investigate the contribution of blood distension to lung mechanics, we studied control mice (normal perfusion), mice after exsanguination (absent perfusion) and mice after varying degrees of parenchymal resection (supra-normal perfusion). On inflation, mean tracheal pressures were higher in the bloodless mouse (4.0α2.5 cmH2O); however, there was minimal difference between conditions on deflation (0.7α0.9 cmH2O). To separate the peripheral and central mechanical effects of blood volume, multi-frequency lung impedance data was fitted to the constant-phase model. The presence or abse…

Nanotechnology, and scaffold implantation for the effective repair of injured organs: An overview on hard tissue engineering

2020

The tissue engineering of hard organs and tissues containing cartilage, teeth, and bones is a widely used and rapidly progressing field. One of the main features of hard organs and tissues is the mineralization of their extracellular matrices (ECM) to enable them to withstand pressure and weight. Recently, a variety of printing strategies have been developed to facilitate hard organ and tissue regeneration. Fundamentals in three-dimensional (3D) printing techniques are rapid prototyping, additive manufacturing, and layered built-up and solid-free construction. This strategy promises to replicate the multifaceted architecture of natural tissues. Nowadays, 3D bioprinting techniques have prove…