Search results for "canine"
showing 10 items of 95 documents
Mutations in DNA Binding and Transactivation Domains Affect the Dynamics of Parvovirus NS1 Protein
2013
ABSTRACT The multifunctional replication protein of autonomous parvoviruses, NS1, is vital for viral genome replication and for the control of viral protein production. Two DNA-interacting domains of NS1, the N-terminal and helicase domains, are necessary for these functions. In addition, the N and C termini of NS1 are required for activation of viral promoter P38. By comparison with the structural and biochemical data from other parvoviruses, we identified potential DNA-interacting amino acid residues from canine parvovirus NS1. The role of the identified amino acids in NS1 binding dynamics was studied by mutagenesis, fluorescence recovery after photobleaching, and computer simulations. Mu…
Drilling dimension effects in early stages of osseointegration and implant stability in a canine model
2015
Background: This study histologically evaluated two implant designs: a classic thread design versus another specifically designed for healing chamber formation placed with two drilling protocols. Material and Methods: Forty dental implants (4.1 mm diameter) with two different macrogeometries were inserted in the tibia of 10 Beagle dogs, and maximum insertion torque was recorded. Drilling techniques were: until 3.75 mm (regular-group); and until 4.0 mm diameter (overdrilling-group) for both implant designs. At 2 and 4 weeks, samples were retrieved and processed for histomorphometric analysis. For torque and BIC (bone-to-implant contact) and BAFO (bone area fraction occupied), a general-linea…
Vertical gingival display changes associated with upper premolars extraction orthodontic treatment : a prospective clinical trial
2020
Background Extraction of upper bicuspids have been anecdotally blamed to increase the vertical gingival display (VGD) anteriorly. However, the extraction may be needed in some cases in order to correct the underlying orthodontic problem. Objectives: To investigate and compare vertical gingival display (VGD) changes associated with upper (first vs second) premolars extraction during orthodontic treatment. Material and Methods Design: A prospective clinical trial. Setting: Postgraduate dental teaching clinics at Jordan University of Science and Technology (JUST). Sample population: Sixty orthodontic patients were included in the study. They were treated with upper first or second premolars ex…
Late steps of parvoviral infection induce changes in cell morphology.
2008
Previously, virus-induced non-filopodial extensions have not been encountered in connection with viral infections. Here, we report emergence of long extensions protruding from Norden laboratory feline kidney (NLFK) and A72 (canine fibroma) cells infected with canine parvovirus for 72 h. These extensions significantly differ in length and number from those appearing in control cells. The most striking feature in the extensions is the length, reaching up to 130 microm, almost twice the average length of a healthy NLFK cell. In A72 cells, the extensions were even longer, up to 200 microm. The results presented here also suggest that the events leading to the growth of these extensions start ea…
Maternal Inheritance of a Recessive RBP4 Defect in Canine Congenital Eye Disease
2018
SUMMARY Maternally skewed transmission of traits has been associated with genomic imprinting and oocyte-derived mRNA. We report canine congenital eye malformations, caused by an amino acid deletion (K12del) near the N terminus of retinol-binding protein (RBP4). The disease is only expressed when both dam and offspring are deletion homozygotes. RBP carries vitamin A (retinol) from hepatic stores to peripheral tissues, including the placenta and developing eye, where it is required to synthesize retinoic acid. Gestational vitamin A deficiency is a known risk factor for ocular birth defects. The K12del mutation disrupts RBP folding in vivo, decreasing its secretion from hepatocytes to serum. T…
In-vivo effects of flapless osteopuncture-facilitated tooth movement in the maxilla and the mandible.
2018
Background This study aimed to investigate the effects of a minimally invasive, flapless procedure to enhance tooth movement in both jaws and to determine whether this triggers the acceleration when repeated monthly. Material and methods The sample consisted of thirty-two individuals whose orthodontic treatment required canine retraction. They were divided into an experimental group and control group. Osteopunctures were performed using orthodontic mini-screws at the distal aspects of the canine teeth at the beginning and on the fourth week of distalization in the experimental group. The control group was treated with conventional mechanics. All canines were retracted. The rates of canine d…
Factors influencing orthodontic treatment time
2021
Ievads: Šī literatūras pārskata mērķis ir aprakstīt faktorus, kuri literatūrā minēti kā ietekmējoši ortodontiskās ārstēšanas ilgumu. Pārskata hipotēze bija tāda, ka ilgāks ārstēšanas laiks ir saistīts ar sarežģītāku diagnozi. Metodes: Šajā pārskatā tika izmantoti 133 literatūras avoti angļu valodā no 1945. līdz 2021. gadam, iesaistot bērnus, pusaudžus un pieaugušos pacientus. Rezultāti: Lielākā daļa autoru ziņo, ka ekstrakcijām, II klases sakodienam, retinētiem kanīniem un faktoriem, kas saistīti ar pacienta atbilstību, bija nozīmīga ietekme uz ārstēšanas laiku. Nav konstatēta saikne starp vecumu un ārstēšanas ilgumu. Secinājumi: P…
Transmigración del canino inferior incluido: Presentación de un caso y revisión de la literatura
2006
La retención, es decir, la no erupción de un diente permanente más allá de un año después de la edad normal de erupción, es relativamente poco frecuente si exceptuamos el caso de los terceros molares y los caninos superiores. La transmigración se define como el fenómeno en el cual un diente incluido no erupcionado traspasa en más de la mitad de su longitud la línea media. Exponemos el caso clínico de una paciente de 20 años de edad, que presentaba la transmigración del canino inferior izquierdo, con un patrón de migración tipo 4 de Mupparapu. De igual forma, realizamos una revisión bibliográfica de los casos publicados de transmigración, actualizando los principales aspectos de esta patolog…
Effect of ATP Binding and Hydrolysis on Dynamics of Canine Parvovirus NS1▿ †
2010
ABSTRACT The replication protein NS1 is essential for genome replication and protein production in parvoviral infection. Many of its functions, including recognition and site-specific nicking of the viral genome, helicase activity, and transactivation of the viral capsid promoter, are dependent on ATP. An ATP-binding pocket resides in the middle of the modular NS1 protein in a superfamily 3 helicase domain. Here we have identified key ATP-binding amino acid residues in canine parvovirus (CPV) NS1 protein and mutated amino acids from the conserved A motif (K406), B motif (E444 and E445), and positively charged region (R508 and R510). All mutations prevented the formation of infectious viruse…
Risultati di un test RFLP su ceppi vaccinali di Canine Distemper Virus in Italia
2013
Canine Distemper (CD) is a highly contagious and multisystemic viral disease of domestic and wild carnivores. A published Restriction Fragment Length Polymorphism (RFLP) test, based on the presence of a PsiI cleavage site on hemagglutinin (H) gene, allows a rapid differentiation of all currently used vaccine strains by virulent field strains. The present study describes the results of this test carried out on different CD vaccines available in Italy in 2010. RFLP has also revealed that the CD strain present in the Vanguard (Pfizer Animal Health) vaccine reacts as a wild-type strain. Moreover, genetic analysis of H gene sequence has showed that Vanguard vaccine strain does not cluster in the…