Search results for "centri"

In-Between ‘Smart’ Urban Growth and ‘Sluggish’ Rural Development? Reframing Population Dynamics in Greece, 1940–2019

2020

Multifaceted demographic dynamics have shaped population growth in Mediterranean Europe, reflecting a metropolitan cycle from urbanization to re-urbanization. To assess the distinctive impact of economic downturns on population dynamics, the present study illustrates the results of an exploratory analysis that assesses urban expansion and rural decline at various temporal scales in Greece, a peripheral country in southeastern Europe. Statistical analysis based on multivariate exploratory techniques outlined the persistent increase of regional populations, evidencing the distinctive role of agglomeration/scale with urbanization and early suburbanization phases (1940&ndash

Changes in the Intra-Metropolitan Location of Producer Services in Ile-de-France (1978-1997) : do information technologies promote a more dispersed s…

2003

Because of their intensive need for face-to-face contacts, producer services have, historically, been found at the core o f CBDs. However, it has been suggested that advances in information technologies could lead to the erosion o f the CBD’s economic base, rendering face-to-face contacts obsolete and enabling producer services to suburbanize. Although a considerable amount o f empirical work has been done on the suburbanization o f these activities in North-America, the same is not true of France. In this paper, we adopt an original methodology to study the role played by face-to-face contacts in the spatial distribution of producer services in the Ile-de-France region between 1978 and 199…

A novel and simple method for the purification of extracellular levansucrase from Zymomonas mobilis.

2003

A new and simple method for the purification of extracellular levansucrase from Zymomonas mobilis from highly viscous fermentation broth was developed. After incubation of the fermentation broth with a fructose-polymer cleaving enzyme preparation (Fructozyme, Novozymes, DK) for 48 h, levansucrase precipitated as aggregates and was redissolved in a 3 M urea solution. By ongoing size-exclusion chromatography on Sephacryl S-300 the final levansucrase preparation was purified 100-fold and exhibited a specific activity of 25-35 U/mg(protein). The levansucrase was stable in 3 M urea solution for at least four months without inactivation. To maximize the enzyme yield the dynamic changes of extrace…

Studies on sea urchin oocytes. II. Synthesis of RNA during oogenesis.

1972

Abstract Isolated oocytes of the sea urchin Paracentrotus lividus actively incorporate 3H-uridine into RNA. Labeled RNA was analysed by sucrose gradient and acrylamide gel electrophoresis following cell fractionation. Much of the radioactivity is incorporated at the nucleolar level in the form of rRNA precursors. The kinetics of maturation of these latter suggests that this occurs at a slower rate than during embryogenesis. Other non-nucleolar RNA classes are also actively labelled and retained in the nucleus for many hours. These results are confirmed by an autoradiographic investigation.

Echovirus 1 Endocytosis into Caveosomes Requires Lipid Rafts, Dynamin II, and Signaling EventsV⃞

2004

Binding of echovirus 1 (EV1, a nonenveloped RNA virus) to the α2β1 integrin on the cell surface is followed by endocytic internalization of the virus together with the receptor. Here, video-enhanced live microscopy revealed the rapid uptake of fluorescently labeled EV1 into mobile, intracellular structures, positive for green fluorescent protein-tagged caveolin-1. Partial colocalization of EV1 with SV40 (SV40) and cholera toxin, known to traffic via caveosomes, demonstrated that the vesicles were caveosomes. The initiation of EV1 infection was dependent on dynamin II, cholesterol, and protein phosphorylation events. Brefeldin A, a drug that prevents SV40 transport, blocked the EV1 infection…

Conspicuous Consumption in Emerging Markets: The Case of Starbucks in Colombia as a Global and Sustainable Brand.

2021

Conspicuous consumption symbolizes ostentatious and material consumption through the purchase of products and services with a higher economic value and that allows their consumers to excel socially. However, conspicuous consumption often does not represent social and environmental values, because their products are often not characterized by being made through sustainable processes. United Nations, through its sustainable development agenda, invited companies to be aware of the social and environmental problems of the world and to be able to generate strategies that can be sustainable over time and allow the growth of both the company and society in general. This study analyses the case of …

Examining the Impact of ICT on Sustainable Development: A Data-Driven Narrative

2021

Considering the challenges of sustainable development and the mixed prescriptions being offered by the use of digital technologies, we present a data-driven narrative of how ICT development impacts the sustainable growth of economies. The analysis is based on historical panel data from 39 economies across the developed and developing economies. The industry-standard CRISP-DM methodology was applied as it is flexible, robust, and offers a practical approach for data analytics. The findings reveal that there are differentiated outcomes in terms of sustainable growth among high-income and low-income economies. This poses legitimate questions as to whether low-income economies will be able to m…

Thirty years of synaptosome research.

1993

Detached synapses (synaptosomes), first isolated by the author in 1958 and identified as such in 1960, are sealed presynaptic nerve terminals often with a portion of the target cell--sometimes amounting to a complete dendritic spine--adhering to their external surface. They can be prepared in high yield from brain tissue and also in decreasing yield from spinal cord, retina, sympathetic ganglia, myenteric plexus and electric organs. They are sealed structures which, under metabolizing conditions, respire, take up oxygen and glucose, extrude Na+, accumulate K+, maintain a normal membrane potential and, on depolarization, release transmitter in a Ca(2+)-dependent manner. They thus provide an …

INDAGINE SU prevalenza, Attitudini E CONOSCENZE Al riguardo Tabagismo TRA GLI STUDENTI DI MEDICINA: UNO STUDIO multicentrico IN ITALIA.

2009

High Speed Stereovision Setup for Position and Motion Estimation of Fertilizer Particles Leaving a Centrifugal Spreader

2014

EA SPE GEAPSI; International audience; A 3D imaging technique using a high speed binocular stereovision system was developed in combination with corresponding image processing algorithms for accurate determination of the parameters of particles leaving the spinning disks of centrifugal fertilizer spreaders. Validation of the stereo-matching algorithm using a virtual 3D stereovision simulator indicated an error of less than 2 pixels for 90% of the particles. The setup was validated using the cylindrical spread pattern of an experimental spreader. A 2D correlation coefficient of 90% and a Relative Error of 27% was found between the experimental results and the (simulated) spread pattern obtai…