Search results for "chino"

Identification of antigenic proteins from Echinostoma caproni (Trematoda) recognized by mouse immunoglobulins M, A and G using an immunoproteomic app…

2008

Antigenic proteins of Echinostoma caproni (Trematoda) against mouse IgM, IgA, IgG, IgG1 and IgG2a were investigated by immunoproteomics. Excretory/secretory products (ESP) of E. caproni separated by two-dimensional (2D) gel electrophoresis were transferred to nitrocellulose membranes and probed with the different mouse immunoglobulin classes. A total of four proteins (enolase, 70 kDa heat-shock protein (HSP-70), actin and aldolase) were accurately identified. Enolase was recognized in eight different spots of which seven of them were detected in the expected molecular weight and were recognized by IgA, IgG or IgG and IgG1. Another spot identified as enolase at 72 kDa was only recognized by …

Screening trematodes for novel intervention targets: a proteomic and immunological comparison of Schistosoma haematobium, Schistosoma bovis and Echin…

2011

SUMMARYWith the current paucity of vaccine targets for parasitic diseases, particularly those in childhood, the aim of this study was to compare protein expression and immune cross-reactivity between the trematodes Schistosoma haematobium, S. bovis and Echinostoma caproni in the hope of identifying novel intervention targets. Native adult parasite proteins were separated by 2-dimensional gel electrophoresis and identified through electrospray ionisation tandem mass spectrometry to produce a reference gel. Proteins from differential gel electrophoresis analyses of the three parasite proteomes were compared and screened against sera from hamsters infected with S. haematobium and E. caproni fo…

Unusual Hydatid Cyst-Like Images Caused by Staphylococcus Lugdunensis Infective Endocarditis

2018

Posaconazole Activity against Candida glabrata after Exposure to Caspofungin or Amphotericin B

2008

ABSTRACT We evaluated the effects of sequential therapy with caspofungin (CAS) or amphotericin B (AMB) followed by posaconazole (POS) against Candida glabrata . The susceptibilities to POS of yeast cells pre-exposed to CAS or AMB were identical to those of untreated cells as shown by standard Clinical and Laboratory Standards Institute broth dilution, cell viability, and disk diffusion methods. We then investigated the activity of sequential regimens in an experimental model of disseminated candidiasis. CAS given at 1 mg/kg/day for 2 days followed by POS at either 15 or 30 mg/kg/day significantly reduced the counts compared to the controls, but this treatment was not superior to the use of …

Kinetics of echinostoma caproni (trematoda: echinostomatidae) antigens in feces and serum of experimentally infected hamsters and rats

2004

This study reports on the kinetics of antibody production to Echinostoma caproni and the dynamics of antigens in feces and sera in 2 experimental hosts (hamsters and rats) that display different degrees of susceptibility with this echinostome. Echinostoma caproniproduced chronic infections in hamsters, whereas rats lost the infection at 49-56 days postinfection (DPI). Hamsters developed higher antibody responses than rats, probably in relation to different intestinal absorptions of worm antigens in each host species. The levels of coproantigens were indicative of the course of infection in each host. Positive coproantigen levels were detected at 1-2 DPI in both hosts, and the values remaine…

A QUANTITATIVE APPROACH TO THE EXPERIMENTAL TRANSMISSION SUCCESS OF ECHINOSTOMA FRIEDI (TREMATODA: ECHINOSTOMATIDAE) IN RATS

2006

Using a range of parameters, the ability of rats (Rattus norvegicus) to successfully transmit Echinostoma friedi to the next host was examined under experimental conditions. The concept of Experimental Transmission Success (TM), defined as the number of hosts that become successfully infected after exposure to a number of infective stages produced by a previous host per unit of inoculation at which this latter host was exposed, was introduced. Using data for the egg output and miracidium hatching and infectivity, the TM permits us to estimate the ability of a particular defintive host species to successfully transmit a parasite species. This concept may be also useful to compare the transmi…

An experimental study of the reproductive success of Echinostoma friedi (Trematoda: Echinostomatidae) in the golden hamster

2003

Viable eggs produced weekly per infective stage was used as a measure of the reproductive success of Echinostoma friedi during the first 12 weeks of infection in hamsters. The weekly reproductive success was not constant during the experiment in relation to the egg output and the proportion of viable eggs produced. The egg release started during week 2 post-inoculation, attaining a maximum during week 3. A decline in egg output was observed from week 9. Viable eggs were only produced from week 3 post-inoculation and a maximum was attained at week 4 of the experiment. A decline in egg viability was observed from week 9. Considering together the egg output and the egg viability, the maximum w…

Helminth parasites of the wolfCanis lupusfrom Latvia

2009

AbstractThirty-four wolves were collected between 2003 and 2008 from throughout Latvia and examined for helminths. A total of 17 helminth species were recorded: the trematodeAlaria alata(85.3%); the cestodesDiphyllobothrium latum(2.9%),Echinococcus granulosus(2.9%),Echinococcus multilocularis(5.9%),Mesocestoides lineatus(5.9%),Taenia crassiceps(8.8%),Taenia hydatigena(41.2%),Taenia (ovis) krabbei(8.8%),Taenia multiceps(47.1%),Taenia pisiformis(20.6%),Taenia polyacantha(11.8%),Taeniaspp. (8.8%); and the nematodesAncylostoma caninum(2.9%),Crenosoma vulpis(9.1%),Eucoleus aerophilus(36.4%),Pearsonema plica(41.4%),Trichinellaspp. (69.7%),Toxocara canis(5.8%), andUncinaria stenocephala(41.2%).Ala…

Altered Protein Expression in the Ileum of Mice Associated with the Development of Chronic Infections with Echinostoma caproni (Trematoda)

2015

Background Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode that has been extensively used as experimental model to investigate the factors determining the expulsion of intestinal helminths or, in contrast, the development of chronic infections. Herein, we analyze the changes in protein expression induced by E. caproni infection in ICR mice, a host of high compatibility in which the parasites develop chronic infections. Methodology/Principal Findings To determine the changes in protein expression, a two-dimensional DIGE approach using protein extracts from the intestine of naïve and infected mice was employed; and spots showing significant differential expression…

Population dynamics of Antarctophthirus microchir (Anoplura: Echinophthiriidae) in pups from South American sea lion, Otaria flavescens, in Northern …

2009

We analysed population dynamics of the louse Antarctophthirus microchir in pups of the South American sea lion, Otaria flavescens, at the Punta León rookery (Argentina) over a period of 2 years. A total of 136 pups were aged and marked at the beginning of the lactation period ashore, then sampled for lice at different times within 30 days. Sampling was restricted to the chest and belly, two sites where lice were especially abundant. This concentration on ventral areas might protect lice from thermal stress in the austral summer. Infestation patterns in pups ≤3 days old suggested that the potential for transmission increased from first nymphs to adults. Population trends of each instar with …