Search results for "chlorophyll"

Crystallization of Light-Harvesting Complex II From Vicia Faba (Fabaceae)

1998

The most abundant chlorophyll binding complex in plants is the intrinsic light-harvesting complex II (LHC II), comprising about half of the total chlorophyll in thylakoid membranes. The structure of LHC II has been determined by electron crystallography, providing a three-dimensional map at 3.4 A (1). Nevertheless, high-resolution structure based on x-ray crystallography is still missing because of the lack of highly ordered 3-D crystals. While delipidation of membrane proteins suitable for high-quality 3-D crystals seems to be a prerequisite, in case of LHC H delipidation leads to a loss of the ability to crystallize. So far, standard purification methods like chromatography have been proo…

Nutri-priming as an efficient means to improve the agronomic performance of molybdenum in common bean (Phaseolus vulgaris L.).

2019

International audience; Microelements play important roles in improving crop productivity and quality. Two traditional methods of providing micronutrients are soil and foliar application, yet the cost involved and phytotoxicity risks poses a major challenge in most global agricultural areas. Nutri-priming represents thus a very promising and viable alternative to ensure that the plants' requirements for micronutrients are met. This paper explores the Mo-priming effects on the germination, growth and yield components of common bean (Phaseolus vulgaris L.). In order to accomplishthis research objective, our study is divided in two phases. The first phase seeks to determine the effects of two …

Biochemical indicators for novel forest decline in spruce

1998

Abstract The impact of air pollution on 24 stands of spruce trees in several regions in Germany was investigated. We looked for evidence of biochemical and physiological change at the level of the photosynthetic thylakoid membranes as well as for changes in the antioxidative system in two year old needles. We observed that, as the chlorophyll content decreases in the needles, the amoung of D1 protein declines far more rapidly in relation to the redox components P700 and cytochrome f. Consequently, the PSII/PSI stoichiometry keeps dropping to pregressively lower, meaning unfavorable, values at the chlorophyll content diminishes. This is particularly the case in the higher elevation character…

Calibration and Validation of Algorithms for the Estimation of Chlorophyll-A in Inland Waters with Sentinel-2

2018

The Ocean Color 2 (OC2), Ocean Color 3 (OC3) and Dall'Olmo three-band (TBDO) algorithms used for the estimation of the chlorophyll-a concentration [Chl-a] have been calibrated and validated for Sentinel-2 spectral bands. Measurements of in situ chlorophyll-a, radiometry and simulations with HydroLight have been used for this purpose within the Ecological Status of AQuatic Systems Satellites (ESAQS) Project to estimate water quality in aquatic systems in Spain. The results show that Sentinel-2 spectral bands are suitable for studying the chlorophyll-a concentration in inland waters. The TBDO algorithm have been applied to Sentinel-2 images and satisfactory results have been obtained in Albuf…

7.2 Restoration responses of 19 lakes: are TP thresholds common?

2011

Remote sensing of sun-induced chlorophyll fluorescence at different scales

2014

In this contribution we present activities and selected results obtained in recent studies and campaigns conducted in the context of the FLuorescence EXplorer (FLEX) mission. FLEX is a candidate mission for the ESA 8th Earth Explorer and large efforts are currently dedicated to the development of an implementation scheme for an accurate mapping of fluorescence from the selected spaceborne sensor and mission configuration. Field and airborne data collected in different experimental campaigns, together with simulated data, have been used to demonstrate the feasibility of fluorescence retrievals and the potential of exploiting high spatial resolution fluorescence maps for a better understandin…

Novel leaf-level measurements of chlorophyll fluorescence for photosynthetic efficiency

2015

Solar induced chlorophyll fluorescence (SIF) from vegetation can now be obtained from satellites as well as ground-based field studies, at select wavelengths associated with atmospheric features. At the leaf level, full spectrum (650–800 nm) chlorophyll emissions (ChlF) can be measured using specialized instrumentation to support interpretation of these SIF observations. We found that ChlF spectra differ for leaf bottoms versus upper leaf surfaces, potentially affecting within-canopy radiative scattering. Our ChlF measurements for leaves of eight tree species (n≥125) obtained during fall 2013 senescence at the Duke Forest in North Carolina, USA and the 2014 growing season (n=72) at the USDA…

Ten Years of Paleoceanographic Studies at ODP Site 963 (Central Mediterranean Sea)

2014

The geographical location, the shape and the circulation pattern makes the Mediterranean Sea an ideal laboratory to study the interplay between different climatic systems, abrupt climate changes and the response of marine ecosystems. The Ocean Drilling Program Site 963 was drilled in the Northwestern part of the Sicily Channel, the sill that divides the western from the eastern Mediterranean Sea. Numerous papers have been published on Site 963 sediments in the last decade, investigating Mediterranean paleoceanographic themes. Here we offer a synthetic framework of these investigations carried out by sub-centennial resolution. We present the whole sequence of suborbital climatic oscillations…

Exploring the spatial relationship between airborne-derived red and far-red sun-induced fluorescence and process-based GPP estimates in a forest ecos…

2019

International audience; Terrestrial gross primary productivity (GPP) plays an essential role in the global carbon cycle, but the quantification of the spatial and temporal variations in photosynthesis is still largely uncertain. Our work aimed to investigate the potential of remote sensing to provide new insights into plant photosynthesis at a fine spatial resolution. This goal was achieved by exploiting high-resolution images acquired with the FLuorescence EXplorer (FLEX) airborne demonstrator HyPlant. The sensor was flown over a mixed forest, and the images collected were elaborated to obtain two independent indicators of plant photosynthesis. First, maps of sun-induced chlorophyll fluore…

Partial purification and initial characterization of phytochrome from the mossAtrichum undulatum P. Beauv. grown in the light.

1988

The extraction and partial purification of phytochrome from light-grownAtrichum undulatum P. Beauv., a chlorophyllous moss, is described. Polyethyleneimine and salt fractionation followed by hydroxyapatite and Affi-gel-blue chromatography were used to separate phytochrome from chlorophyll, and to purify the pigment. All steps were performed in the presence of Triton X-100 which improved the yield by a factor of about three. The protein has a molecular weight some-what larger than that ofAvena phytochrome (124 kDa), as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis. It cross-reacts with a monoclonal antibody against phytochrome from etiolated …