6533b7d1fe1ef96bd125d941
RESEARCH PRODUCT
Partial purification and initial characterization of phytochrome from the mossAtrichum undulatum P. Beauv. grown in the light.
Elmar HartmannSilvia E. BraslavskyKurt SchaffnerPeter Lindemannsubject
Gel electrophoresisfood.ingredientbiologyPhytochromefood and beveragesPlant Sciencechemistry.chemical_compoundPigmentAvenafoodchemistryBiochemistryPolyclonal antibodiesChromoproteinChlorophyllvisual_artEtiolationGeneticsbiology.proteinvisual_art.visual_art_mediumdescription
The extraction and partial purification of phytochrome from light-grownAtrichum undulatum P. Beauv., a chlorophyllous moss, is described. Polyethyleneimine and salt fractionation followed by hydroxyapatite and Affi-gel-blue chromatography were used to separate phytochrome from chlorophyll, and to purify the pigment. All steps were performed in the presence of Triton X-100 which improved the yield by a factor of about three. The protein has a molecular weight some-what larger than that ofAvena phytochrome (124 kDa), as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis. It cross-reacts with a monoclonal antibody against phytochrome from etiolated corn (Zea) and a polyclonal antibody against phytochrome from etiolated oat (Avena), and its photoreversibility is similar to that of phytochrome from greenAvena.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1988-12-28 | Planta |