Search results for "chromosome"
showing 10 items of 1175 documents
Spanish human proteome project: Dissection of chromosome 16
2013
11 páginas, 6 figuras.-- et al.
Surfing transcriptomic landscapes. A step beyond the annotation of chromosome 16 proteome
2013
All participating laboratories are members of ProteoRed-ISCIII.-- et al.
Chemically induced chromosome damage in early-developing embryos ofAnilocra physodes L. (Crustacea, Isopoda) following exposure to bis[Dimethyltin(IV…
1993
In order to obtain chromosome preparations from early-developing embryos of Anilocra physodes, a squash technique has been successfully employed. Results gathered after exposure of this material to bis[dimethyltin(IV)chloro]protoporphyrin IX {[(CH3)2SnCl]2 - Protoporphyrin IX} solutions at different exposure times suggest that this chemical complex is capable of producing abnormal metaphase and anaphase figures in proportion to its concentration and not to exposure length. Essentially, all of the chromosome abnormalities are classifiable as chromosome fragments mainly observed at the metaphase stage; chromosome bridges; and large decondensed chromosome regions.
ChemInform Abstract: Chemically Induced Chromosome Damage in Early-Developing Embryos of Anilocra Physodes L. (Crustacea, Isopoda) Following Exposure…
2010
In order to obtain chromosome preparations from early-developing embryos of Anilocra physodes, a squash technique has been successfully employed. Results gathered after exposure of this material to bis[dimethyltin(IV)chloro]protoporphyrin IX {[(CH3)2SnCl]2 - Protoporphyrin IX} solutions at different exposure times suggest that this chemical complex is capable of producing abnormal metaphase and anaphase figures in proportion to its concentration and not to exposure length. Essentially, all of the chromosome abnormalities are classifiable as chromosome fragments mainly observed at the metaphase stage; chromosome bridges; and large decondensed chromosome regions.
GH successful treatment in a female with a de novo 46,XX,add(X)(p36),t(X;Y)(p36.3;p11.2), growth impairment and SHOX-haploinsufficiency
2019
Abstract Children with chromosome translocations, concerning X chromosome, have a genetic pattern different from Turner syndrome; however, when a translocation involves the of part of X chromosome including short stature homeobox-containing Sex-determining Region Y gene, growth may be severely compromised. We describe the clinical case of a 2.2-year-old-female, arrived at our paediatric unit for a decrease of height velocity. The karyotype was 46,XX,add(X)(p36.3). Array comparative genomic hybridization showed a fragment of Y chromosome, extended from 8.803.981 (Yp11.2) to 28.767.604 (Yq11.23). The final karyotype was 46,XX,add(X)(p36),t(X;Y)(p36.3;p11.2). Fluorescence in situ Hybridization…
Development of an Italian RM Y-STR haplotype database: Results of the 2013 GEFI collaborative exercise.
2015
Recently introduced rapidly mutating Y-chromosomal short tandem repeat (RM Y-STR) loci, displaying a multiple-fold higher mutation rate relative to any other Y-STRs, including those conventionally used in forensic casework, have been demonstrated to improve the resolution of male lineage differentiation and to allow male relative separation usually impossible with standard Y-STRs. However, large and geographically-detailed frequency haplotype databases are required to estimate the statistical weight of RM Y-STR haplotype matches if observed in forensic casework. With this in mind, the Italian Working Group (GEFI) of the International Society for Forensic Genetics launched a collaborative ex…
Molecular modelling and QSAR in the discovery of HIV-1 integrase inhibitors
2007
The treatment regimens for the HIV-1 have mainly included reverse transcriptase or protease inhibitors but their long-term clinical utility is limited by severe side effects and viral drug resistance. A new attractive target for chemotherapeutic intervention can be the Integrase enzyme, that mediates the integration of HIV-1 DNA into a host chromosome, for which there is no known counterparts in the host cell. A number of derivatives have been found to inhibit IN in in vitro assays, but no successful drug based on them has emerged so far, although many compounds have been proposed. Moreover most of the inhibitors do not belong to a very precise structural class: this fact makes these compou…
Comparative cytogenetics of human chromosome 3q21.3 reveals a hot spot for ectopic recombination in hominoid evolution
2004
Fluorescence in situ hybridization mapping of fully integrated human BAC clones to primate chromosomes, combined with precise breakpoint localization by PCR analysis of flow-sorted chromosomes, was used to analyze the evolutionary rearrangements of the human 3q21.3-syntenic region in orangutan, siamang gibbon, and silvered-leaf monkey. Three independent evolutionary breakpoints were localized within a 230-kb segment contained in BACs RP11-93K22 and RP11-77P16. Approximately 200 kb of the human 3q21.3 sequence was not present on the homologous orangutan, siamang, and Old World monkey chromosomes, suggesting a genomic DNA insertion into the breakpoint region in the lineage leading to humans a…
Fine analysis of the chromatin structure of the yeast SUC2 gene and of its changes upon derepression. Comparison between the chromosomal and plasmid-…
1987
Micrococcal nuclease digestion has been used to investigate some fine details of the chromatin structure of the yeast SUC2 gene for invertase. Precisely positioned nucleosomes have been found on a 2 kb sequence from the 3' non-coding region, and four nucleosomes also seem to occupy fixed positions on the 5' flank. Eleven nucleosomes lie on the coding region, although their positioning is not as precise as in the flanks. When the gene is derepressed, these latter nucleosomes adopt a more open conformation and so do two of the nucleosomes positioned on the 5' flank. A dramatic change occurs in the 3' flank, whose involvement in the structural transitions of chromatin upon gene activation is p…
A new Multi-Layers Method to Analyze Gene Expression
2007
In the paper a new Multi-Layers approach (called Multi-Layers Model MLM) for the analysis of stochastic signals and its application to the analysis of gene expression data is presented. It consists in the generation of sub-samples from the input signal by applying a threshold technique based on cut-set optimal conditions. The MLM has been applied on synthetic and real microarray data for the identification of particular regions across DNA called nucleosomes and linkers. Nucleosomes are the fundamental repeating subunits of all eukaryotic chromatin, and their positioning provides useful information regarding the regulation of gene expression in eukaryotic cells. Results have shown a good rec…