Search results for "co-colture"
showing 2 items of 2 documents
HSP90 AND ENOS PARTIALLY CO-LOCALIZE AND CHANGE CELLULAR LOCALIZATION IN RELATION TO DIFFERENT ECM COMPONENTS IN 2D AND 3D CULTURES OF ADULT RAT CARD…
2007
BACKGROUND INFORMATION: Cultivation techniques promoting three-dimensional organization of mammalian cells are of increasing interest, since they confer key functionalities of the native ECM (extracellular matrix) with a power for regenerative medicine applications. Since ECM compliance influences a number of cell functions, Matrigel-based gels have become attractive tools, because of the ease with which their mechanical properties can be controlled. In the present study, we took advantage of the chemical and mechanical tunability of commonly used cell culture substrates, and co-cultures to evaluate, on both two- and three-dimensional cultivated adult rat cardiomyocytes, the impact of ECM c…
In vitro cellular co-colture method to induce immunological tolerance
2017
An in vitro method able to modulate peripheral blood mononuclear cells of patients with auto-immune organ-specific diseases is described. The protocol makes auto-reactive lymphocytes tolerogenic through a process of re-education in recognition of self-antigen after co-culture with heterologous and/or autologous fibroblast limbal stem cells.