Search results for "coding"
showing 10 items of 920 documents
Generation of reporter plasmids containing defined base modifications in the DNA strand of choice
2012
Physiological effects of DNA bases other than A, G, C, and T as well as ways of removal of such bases from genomes are studied intensely. Methods for targeted insertion of modified bases into DNA, therefore, are highly demanded in the fields of DNA repair and epigenetics. This article describes efficient procedures for incorporation of modified DNA bases into a plasmid-borne enhanced green fluorescent protein (EGFP) gene. The procedure exploits excision of a stretch of 18 nt from either the transcribed or nontranscribed DNA strand with the help of the sequence-specific nicking endonucleases Nb.Bpu10I and Nt.Bpu10I. The excised single-stranded oligonucleotide is then swapped for a synthetic …
8-Oxo-7,8-dihydroguanine in DNA does not constitute a barrier to transcription, but is converted into transcription-blocking damage by OGG1.
2011
The common DNA base modification 8-oxo-7,8-dihydroguanine (8-oxo-G) affects the efficiency and fidelity of transcription. We constructed plasmid substrates carrying single 8-oxo-G residues, specifically positioned in the transcribed or the non-transcribed DNA strands, to investigate their effects on the expression of an EGFP reporter gene and to explore the role of base excision repair in the mechanism of transcription inhibition. We report that 8-oxo-G does not directly block transcription in cells, since a single 8-oxo-G in the transcribed DNA strand did not reduce the EGFP expression levels in repair-deficient (OGG1-null) mouse embryonic fibroblast cell lines. Rather, inhibition of trans…
A novel hardware accelerator for the HEVC intra prediction
2015
International audience; A novel hardware accelerator for the High Efficiency Video Coding (HEVC) intra prediction is presented in this paper in order to reduce the computation complexity within this standard and to accelerate the concerned calculations. We propose a new pipelined structure that we called Processing Element (PE) to execute all angular modes, and we repeat it in five paths that our architecture composed of. We present also another structure to carry out the Planar mode. This architecture supports all intra prediction modes for all prediction unit sizes. The synthesis results show that our design can run at 213 MHz for Xilinx Virtex 6 and is capable to process real time 120 10…
Fully pipelined real time hardware solution for High Efficiency Video Coding (HEVC) intra prediction
2016
International audience; A fully pipelined hardware accelerator for the High Efficiency Video Coding (HEVC) intra prediction is presented in this paper in order to reduce the computation complexity coming with this module and to accelerate the concerned calculations. Two reconfigurable structures are developed in this paper, the first one concerns angular modes and is identified as Processing Element for Angular (PEA) modes, the other is made in order to handle with the Planar mode and is identified as Processing Element for the Planar (PEP) mode. Each structure is repeated in five paths, that our architecture composed of, working in parallel way. This architecture supports all intra predict…
A HARDWARE SOLUTION FOR HEVC INTRA PREDICTION LOSSLESS CODING
2015
International audience; The lossless coding mode of the High Efficiency Video Coding (HEVC) main profile that bypasses transform, quantization, and in-loop filters is described. Compared to the HEVC non-lossless coding mode, the HEVC lossless coding mode provides perfect fidelity and an average bit-rate reduction of 3.2%–13.2%. It also significantly outperforms the existing lossless compression solutions, such as JPEG2000 and JPEG-LS for images as well as WinRAR for data archiving. A fully parallel-based solution is presented in this paper in order to reduce processing time and computation complexity resulting from intra prediction. Two higher performance structures are designed to perform …
Ligand binding study revealing a human olfactory receptor involved in waxy-floral odor perception
2006
International audience
The time course of processing handwritten words: An ERP investigation
2021
Available online 25 June 2021. Behavioral studies have shown that the legibility of handwritten script hinders visual word recognition. Furthermore, when compared with printed words, lexical effects (e.g., word-frequency effect) are magnified for less intelligible (difficult) handwriting (Barnhart and Goldinger, 2010; Perea et al., 2016). This boost has been interpreted in terms of greater influence of top-down mechanisms during visual word recognition. In the present experiment, we registered the participants’ ERPs to uncover top-down processing effects on early perceptual encoding. Participants’ behavioral and EEG responses were recorded to high- and low-frequency words that varied in scr…
An Analysis of Flash Page Reuse With WOM Codes
2018
Flash memory is prevalent in modern servers and devices. Coupled with the scaling down of flash technology, the popularity of flash memory motivates the search for methods to increase flash reliability and lifetime. Erasures are the dominant cause of flash cell wear, but reducing them is challenging because flash is a write-once medium— memory cells must be erased prior to writing. An approach that has recently received considerable attention relies on write-once memory (WOM) codes, designed to accommodate additional writes on write-once media. However, the techniques proposed for reusing flash pages with WOM codes are limited in their scope. Many focus on the coding theory alone, whereas o…
kmcEx
2019
Memory-frugal and retrieval-efficient encoding of counted k-mers.