Search results for "coding"
showing 10 items of 920 documents
Fine analysis of the chromatin structure of the yeast SUC2 gene and of its changes upon derepression. Comparison between the chromosomal and plasmid-…
1987
Micrococcal nuclease digestion has been used to investigate some fine details of the chromatin structure of the yeast SUC2 gene for invertase. Precisely positioned nucleosomes have been found on a 2 kb sequence from the 3' non-coding region, and four nucleosomes also seem to occupy fixed positions on the 5' flank. Eleven nucleosomes lie on the coding region, although their positioning is not as precise as in the flanks. When the gene is derepressed, these latter nucleosomes adopt a more open conformation and so do two of the nucleosomes positioned on the 5' flank. A dramatic change occurs in the 3' flank, whose involvement in the structural transitions of chromatin upon gene activation is p…
Role of non-coding RNAs as biomarkers of deleterious cardiovascular effects in sepsis.
2021
The mechanisms occurring during sepsis that produce an increased risk of cardiovascular (CV) disease (CVD) are poorly understood. Even less information exists regarding CV dysfunction as a complication of sepsis, particularly for sepsis-induced cardiomyopathy. However, recent research has demonstrated that non-coding RNAs, including microRNAs, long non-coding RNAs, and circular RNAs, play a crucial role in genetic reprogramming, gene regulation, and inflammation during the development of CVD. Here we describe experimental findings showing the importance of non-coding RNAs mediating relevant mechanisms underlying CV dysfunction after sepsis, so contributing to sepsis-induced cardiomyopathy. …
Messenger RNA degradation is initiated at the 5′ end and follows sequence- and condition-dependent modes in chloroplasts
2011
Using reporter gene constructs, consisting of the bacterial uidA (GUS) coding region flanked by the 5' and 3' regions of the Chlamydomonas rbcL and psaB genes, respectively, we studied the degradation of mRNAs in the chloroplast of Chlamydomonas reinhardtii in vivo. Extending the 5' terminus of transcripts of the reporter gene by more than 6 nucleotides triggered rapid degradation. Placing a poly(G) tract, known to pause exoribonucleases, in various positions downstream of the 5' terminus blocked rapid degradation of the transcripts. In all these cases the 5' ends of the accumulating GUS transcripts were found to be trimmed to the 5' end of the poly(G) tracts indicating that a 5' → 3' exori…
Translational read-through as an alternative approach for ocular gene therapy of retinal dystrophies caused by in-frame nonsense mutations
2014
AbstractThe eye has become an excellent target for gene therapy, and gene augmentation therapy of inherited retinal disorders has made major progress in recent years. Nevertheless, a recent study indicated that gene augmentation intervention might not stop the progression of retinal degeneration in patients. In addition, for many genes, viral-mediated gene augmentation is currently not feasible due to gene size and limited packaging capacity of viral vectors as well as expression of various heterogeneous isoforms of the target gene. Thus, alternative gene-based strategies to stop or delay the retinal degeneration are necessary. This review focuses on an alternative pharmacologic treatment s…
Recensione a “Goschler J., Stefanowitsch A. (eds.), Variation and change in the encoding of motion events, Human Cognitive Processing (HCP) 41, John …
2016
Variation and change in the encoding of motion events is a thematic collective volume focused on motion event encoding, one of the main topics in Cognitive Linguistics. Edited by Juliana Goschler and Anatol Stefanowitsch, it is the forty-first volume of the Human Cognitive Processing series, a John Benjamins’ interdisciplinary book series concerning research on language(s) and human cognitive faculties. The book results from a theme session of the Fourth International Conference of the German Cognitive Linguistic Association.
Engineering a Circular Riboregulator in Escherichia coli
2020
RNAs of different shapes and sizes, natural or synthetic, can regulate gene expression in prokaryotes and eukaryotes. Circular RNAs have recently appeared to be more widespread than previously thought, but their role in prokaryotes remains elusive. Here, by inserting a riboregulatory sequence within a group I permuted intron-exon ribozyme, we created a small noncoding RNA that self-splices to produce a circular riboregulator in Escherichia coli . We showed that the resulting riboregulator can trans -activate gene expression by interacting with a cis -repressed messenger RNA. We characterized the system with a fluorescent reporter and with an antibiotic resistance marker, and we modeled thi…
A TDMA-Based MAC Protocol Supporting Cooperative Communications in Wireless Mesh Networks
2011
This paper proposes a TDMA-based medium access control protocol which enables cooperative communications in multi-hop wireless mesh networks. According to the proposed scheme, each router at the two-hop neighbourhood of each other is allocated to a specific time slot for accommodating either direct or cooperative transmissions in a coordinated manner, controlled by mini-slots which are part of the time slot. Benefiting from the elaborate mini-slot design, channel resources are fairly and efficiently allocated to each router so that no handshake is needed prior to each packet transmission. By providing access priority to cooperative transmission through an optimal relay which is determined b…
A mini-slot-based cooperative MAC protocol for wireless mesh networks
2010
This paper proposes a TDMA-based medium access control protocol with cooperative communications enabled in wireless mesh networks. The proposed scheme is based on a multi-hop scenario which is extended from the conventional one-hop triangle cooperative transmission scenario. Benefiting from the elaborate mini-slot design, channel resources are fairly and efficiently allocated to each router so that no handshake is needed prior to every packet transmission. By giving priority for channel access to cooperative transmission performed by an optimal helper which is determined based on the received SNR, higher successful transmission rate can be achieved. Numerical results demonstrate that the pr…
Throughput and energy efficiency comparison of one-hop, two-hop, virtual relay and cooperative retransmission schemes
2010
Two main types of approaches exist for implementing cooperative communications at the MAC layer: virtual-hop relay and cooperative retransmission. While the virtual-hop relay schemes employ relay nodes to forward packets when higher end-to-end throughput can be achieved compared with the direct transmission, the cooperative retransmission schemes use relays to retransmit data only after the direct transmission fails. However, the performance of the these different approaches has not been compared in the literature, especially when energy efficiency is considered. In order to find out the best transmission scheme, this paper evaluates and compares the performance of the one-hop direct transm…
A contribution to the characterization of Ruppia drepanensis (Ruppiaceae), a key species of threatened Mediterranean wetlands
2021
To elucidate the taxonomic status of Ruppia drepanensis Tineo ex Guss. (Alismatales, Ruppiaceae), we performed morphological analysis and DNA barcoding of historical materials (including the lectotype) and fresh samples (including those from a recently discovered population near the locus classicus in Sicily, Italy). We conclude that R. drepanensis is a separate species, closely related to R. spiralis L. ex Dumort., that occurs in temporary inland waters from the western to central sectors of the Mediterranean region. We also highlight the importance of vouchers and the need to link molecular investigations to field, ecological, and morphological investigations.