Search results for "complex"
showing 10 items of 5889 documents
Splenic Marginal Zone Lymphoma Shows a Distinct Pattern of DNA Copy Number Aberrations That Correlates with Tumor Characteristics and Predicts Diseas…
2006
Abstract Splenic marginal zone lymphoma (SMZL) is an indolent B cell malignancy whose diagnosis is based on lymphocyte morphology, immunophenotype and marrow and/or splenic histology. Unlike other lymphomas, there is not a common chromosomal translocation specific for SMZL, and genetic prognostic factors are poorly defined. To investigate the pattern of genomic aberrations in SMZL, we applied comparative genomic hybridization to BAC microarrays (array CGH) to a well characterized series of 75 SMZL specimens. We applied two different 1 Mb-resolution BAC arrays: UCSF HumArray 3.2 and a novel array CGH platform developed at Univ. of Salamanca. These arrays allowed us to detect DNA copy number …
Selective labelling of melittin with a fluorescent dansylcadaverine probe using guinea-pig liver transglutaminase
1991
Abstract Melittin, a C-terminal peptide, incorporated the fluorescent probe monodansylcadaverine (DNC) when catalysed by guinea-pig liver transglutaminase and Ca2+, as determined by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). A 1:1 adduct DNC-melittin was identified in which a single glutamine residue out of two, i.e. Gln25, acts as acyl donor. Incubation of melittin with transglutaminase in the absence of DNC originated high molecular mass complexes indicative that the peptide lysine residue can act as an acyl acceptor. The DNC-melittin was about 3 times more active in the lysis of red cell membranes than native melittin. Fluorescence study of the lab…
Proteasome comprising a beta1 inducible subunit acts as a negative regulator of NADPH oxidase during elicitation of plant defense reactions.
2005
Elicitation of defense reactions in tobacco by cryptogein, triggered a production of active oxygen species (AOS) via the NADPH oxidase, NtrbohD, and an accumulation of beta1din, a defense induced beta-type subunit of 20S proteasome. The proteasome inhibitor, MG132, stimulated this AOS production. Tobacco cells transformed with sense constructs of beta1din showed an inhibition of the AOS production following elicitin treatment, whereas the antisense transformed cells showed a strongly enhanced AOS production. In cells transformed with sense construct of beta1din, the NtrbohD transcripts failed to be induced by cryptogein as observed in control and antisense transformed cells. Conversely, in …
Nano into Micro Formulations of Tobramycin for the Treatment of Pseudomonas aeruginosa Infections in Cystic Fibrosis.
2017
Here, nano into micro formulations (NiMs) of tobramycin for the treatment of Pseudomonas aeruginosa airway infections in cystic fibrosis (CF) are described. NiMs were produced by spray drying a solution containing polymers or sugars and a nanometric polyanion–tobramcyin complex (PTC), able to achieve a prolonged antibiotic release. NiMs properties were compared to TOBIPodhaler(Novartis), the only one commercially available dry powder inhalatory formulation based on porous microparticles. Produced NiMs showed adequate characteristics for pulmonary administration, as spherical shape, micrometric size, and high cytocompatibility toward human bronchial epithelial cells. Contrarily to TOBIPodhal…
Chloroplast signal length requirement reflects the outer membrane and TOC complex dimension
2015
Background and Purpose: The evolution of an efficient preprotein targeting and translocation system was a central prerequisite for the endosymbiotic integration of a -proteobacteria and cyanobacteria as cellular organelles. Today, it is widely accepted that during evolution most (pre-)proteins destined for these two organelles were equipped with an N-terminal targeting signal for localization. While multiple modes of evolution of these extensions are currently discussed, all evolved signals serve the same function – forming a signal for targeting to the correct organelle and translocation across both membranes. We aimed to generalize the current idea for the length requirement of the N-term…
Multi-label Classification Using Stacked Hierarchical Dirichlet Processes with Reduced Sampling Complexity
2018
Nonparametric topic models based on hierarchical Dirichlet processes (HDPs) allow for the number of topics to be automatically discovered from the data. The computational complexity of standard Gibbs sampling techniques for model training is linear in the number of topics. Recently, it was reduced to be linear in the number of topics per word using a technique called alias sampling combined with Metropolis Hastings (MH) sampling. We propose a different proposal distribution for the MH step based on the observation that distributions on the upper hierarchy level change slower than the document-specific distributions at the lower level. This reduces the sampling complexity, making it linear i…
Lévy flights in confining potentials.
2009
We analyze confining mechanisms for L\'{e}vy flights. When they evolve in suitable external potentials their variance may exist and show signatures of a superdiffusive transport. Two classes of stochastic jump - type processes are considered: those driven by Langevin equation with L\'{e}vy noise and those, named by us topological L\'{e}vy processes (occurring in systems with topological complexity like folded polymers or complex networks and generically in inhomogeneous media), whose Langevin representation is unknown and possibly nonexistent. Our major finding is that both above classes of processes stay in affinity and may share common stationary (eventually asymptotic) probability densit…
Volume-convergent sequences of Haken 3-manifolds
2003
Abstract Let M be a closed orientable 3-manifold and let Vol(M) denote its Gromov simplicial volume. This paper is devoted to the study of sequences of non-zero degree maps f i :M→N i to Haken manifolds. We prove that any sequence of Haken manifolds (Ni,fi), satisfying limi→∞deg(fi)×Vol(Ni)=Vol(M) is finite up to homeomorphism. As an application, we deduce from this fact that any closed orientable 3-manifold with zero Gromov simplicial volume and in particular any graph manifold dominates at most finitely many Haken 3-manifolds. To cite this article: P. Derbez, C. R. Acad. Sci. Paris, Ser. I 336 (2003).
LEM for twisted re-entrant angle sections
2014
In this paper an innovative numerical method named as line element-less method, LEM, for finding solution of torsion problem has been extended to all shaped sections, including sections possessing re-entrant angles at their boundary. The response solution in terms of shear stress field or Prandtl function or warping function in all domain and for any kind of domain with arbitrary contour, may be performed quickly, calculating line integrals only. The method takes full advantage of the theory of analytic complex function and is robust in the sense that returns exact solution if this exists. Numerical implementation of LEM has been developed using Mathematica software without resorting to any…
Nearly zero ultraflattened dispersion in photonic crystal fibers.
2000
We present a procedure for achieving photonic crystal fibers with nearly zero ultraflattened group-velocity dispersion. Systematic knowledge of the special guiding properties of these fibers permits the achievement of qualitatively novel dispersion curves. Unlike the behavior of conventional fibers, this new type of dispersion behavior permits remarkably improved suppression of third-order dispersion, particularly in the low-dispersion domain.