Search results for "crisp"
showing 10 items of 104 documents
Mboat7 down-regulation by hyper-insulinemia induces fat accumulation in hepatocytes.
2020
Background: Naturally occurring variation in Membrane-bound O-acyltransferase domain-containing 7 (MBOAT7), encoding for an enzyme involved in phosphatidylinositol acyl-chain remodelling, has been associated with fatty liver and hepatic disorders. Here, we examined the relationship between hepatic Mboat7 down-regulation and fat accumulation. Methods: Hepatic MBOAT7 expression was surveyed in 119 obese individuals and in experimental models. MBOAT7 was acutely silenced by antisense oligonucleotides in C57Bl/6 mice, and by CRISPR/Cas9 in HepG2 hepatocytes. Findings: In obese individuals, hepatic MBOAT7 mRNA decreased from normal liver to steatohepatitis, independently of diabetes, inflammatio…
Selection of potential probiotic lactobacilli from pig feces to be used as additives in pelleted feeding
2006
Thirty-five isolates from pig feces were identified as Lactobacillus reuteri (12 strains), Lactobacillus mucosae (7), Lactobacillus plantarum (6), Lactobacillus kitasatonis (3), Lactobacillus rossiae (2), Lactobacillus ultunensis (2), Lactobacillus crispatus (2), and Lactobacillus intestinalis (1) by partial sequence analysis of the 16S rRNA. All isolates were detected at 8-9 log CFU g(-1). Preliminarily, strains were selected based on resistance to heat treatments (ca. 70 degrees C for 10 s). The decrease in viability for some L. reuteri, L. mucosae, L. plantarum, L. kitasatonis, and L. rossiae strains was lower than 1 log cycle. Selected strains were further characterized for acid and bil…
Citrus biotechnology
2020
Agricultural crops that can better withstand the changing climatic and pathogen landscape have been produced through natural selection throughout the millennia and, in recent years, through the process of human-assisted plant breeding and selection. However, a lack of genetic diversity in many commercially cultivated crops (due to monoculture) has made them more vulnerable to biotic and abiotic stresses (Esquinas-Alcazar, 2005; Keneni et al., 2012). Citrus, belonging to the Rutaceae family, is one of the most important commercial woody fruit crops in the tropical and subtropical areas of the world. The origin of citrus is traced back to parts of tropical and subtropical Southeast Asia (Wu e…
Fruit quality of Sicilian, commercially affirmed, and newly selected apple varieties: potential resources for genetic improvement and specialty marke…
2012
Several apple varieties are constantly selected for improved quality traits and introduced for cultivation and marketing in addition to a few traditional and affirmed varieties. Recently, specific fruit quality traits like enhanced flavor, crispness and juiciness have been particularly requested by consumers and are gradually replacing more conventional characteristics like fruit size, peel color and sweetness in the breeding programs. Furthermore, some of the newly selected varieties exhibit a relatively low level of plasticity, posing serious problems of adaptation when cultivation is proposed in a wide range of environments. In this most recent perspective, some of the old varieties or l…
Chi dice che i batteri fanno male? I CRISPR ed il loro potenziale genetico. In Le scienze naturali nella scuola
2019
L’immaginario collettivo non ha un’opinione buona e nemmeno benevola sui batteri. Sono spesso legati a concetti di mancanza di igiene, malattie ed a volte anche a situazioni più gravi financo la morte. È indubbio che alcuni generi di procarioti sono agenti patogeni di specifiche malattie infettive che, in passato più che oggi, hanno mietuto vittime nel genere umano. Ma che dire dei batteri lattici degli yogurt o della nostra flora batterica intestinale? C’è da aggiungere anche una lunga serie di organismi procarioti, non patogeni, che hanno, negli ultimi due secoli, costituito sistemi biologici idonei allo studio della biologia molecolare e della genetica molecolare. In definitiva è molto p…
Investigating CRISPR-CAS13b as a tool for the RNA editing of CFTR mRNA with premature stop codon
2020
Background and Rationale Some CF patients are compound heterozygous or homozygous for nonsense mutations in the CFTR gene. Mutant CFTR gene coding for transcripts with premature termination codons (PTCs) is responsible for truncated CFTR protein and for a severe form of the disease. In a precision medicine framework the “REPAIRv2” (RNA Editing for Programmable A to I Replacement v2) tool, developed in the laboratory of Dr. Feng Zhang (USA), seems a good alternative to restore the full-length CFTR protein by editing its mRNA containing PTCs. This new approach is based on the possibility of targeting a deaminase enzyme (huADAR2) to a specific Adenosine, to be edited to Inosine (G analogue), o…
I fertili 0ttant'anni di Enrico Crispolti
2013
Articolo di critica d'arte sull'attività dello storico dell'arte E. Crispolti.
Francesco Crispo Moncada e la Venezia Giulia (1919-1924)
2018
Francesco Crispo Moncada capo della polizia
Fare un monumento di se stesso. Una fonte oratoria
2010
La retorica politica di Francesco Crispi
Giovanni Maurigi, un allievo illustre di Emerico Amari. Storia e politica nella Sicilia risorgimentale
2011
Giovanni Maurigi (1823-1881) was a very well known Sicilian jurist. Student of philosopher Emerico Amari, his maestro's intellectual stimulus led him to contribute to the 1848 risings in Palermo. He was a well known laywer during the Borbonic regime, who defended patriots persecuted by the government. After Italian unification he became a judge. A liberal, his own professional career can be interpreted as an example of Sicilian elite recruiting in the new unified state.