Search results for "crosstalk"
showing 7 items of 77 documents
24-(S)-hydroxycholesterol is a potent modulator of cholesterol metabolism in rat retinal Müller glia
2019
International audience
Inhibition of TSH/IGF-1 Receptor Crosstalk by Teprotumumab as a Treatment Modality of Thyroid Eye Disease
2021
Abstract Context We previously presented evidence that TSH receptor (TSHR)-stimulating autoantibodies (TSAbs) bind to and activate TSHRs but do not bind to IGF1 receptors (IGF1Rs). Nevertheless, we showed that IGF1Rs were involved in thyroid eye disease (TED) pathogenesis because TSAbs activated crosstalk between TSHR and IGF1R. Teprotumumab, originally generated to inhibit IGF1 binding to IGF1R, was recently approved for the treatment of TED (Tepezza). Objective To investigate the role of TSHR/IGF1R crosstalk in teprotumumab treatment of TED. Design We used orbital fibroblasts from patients with TED (TEDOFs) and measured stimulated hyaluronan (HA) secretion as a measure of orbital fibrobla…
Maternal-fetal cross-talk: elucidating the role of mir-30d in endometrial receptivity and pregnancy outcome
2018
Introducción En el proceso de implantación embrionaria se produce una relación sincrónica entre el endometrio y el embrión que resulta fundamental para su correcta consecución; dicha sincronía se mantiene durante la gestación gracias a la existencia de una comunicación bidireccional entre la madre y el feto basada en la secreción de señales específicas que permiten regular un desarrollo cooperativo. Efectivamente, se ha observado como el embrión libera moléculas específicas del estado gestacional (ej.: gonadotropina coriónica humana o interferón tau en rumiantes), para prevenir la luteólisis inducida por la prostaglandina F2alpha, posibilitando la secreción continua de progesterona durante …
3 ′-5 ′ crosstalk contributes to transcriptional bursting
2019
Abstract Background Transcription in mammalian cells is a complex stochastic process involving shuttling of polymerase between genes and phase-separated liquid condensates. It occurs in bursts, which results in vastly different numbers of an mRNA species in isogenic cell populations. Several factors contributing to transcriptional bursting have been identified, usually classified as intrinsic, in other words local to single genes, or extrinsic, relating to the macroscopic state of the cell. However, some possible contributors have not been explored yet. Here, we focus on processes at the 3 ′ and 5 ′ ends of a gene that enable reinitiation of transcription upon termination. Results Using Bay…
Xrn1 influence on gene transcription results from the combination of general effects on elongating RNA pol II and gene-specific chromatin configurati…
2020
mRNA homoeostasis is favoured by crosstalk between transcription and degradation machineries. Both the Ccr4-Not and the Xrn1-decaysome complexes have been described to influence transcription. While Ccr4-Not has been shown to directly stimulate transcription elongation, the information available on how Xrn1 influences transcription is scarce and contradictory. In this study we have addressed this issue by mapping RNA polymerase II (RNA pol II) at high resolution, using CRAC and BioGRO-seq techniques in Saccharomyces cerevisiae. We found significant effects of Xrn1 perturbation on RNA pol II profiles across the genome. RNA pol II profiles at 5ʹ exhibited significant alterations that were com…
Extracellular vesicles in the human maternal-embryo crosstalk during the preconception period
2020
ANTECEDENTES: nuestro grupo demostró que el endometrio humano secreta exosomas (EXOs) al líquido endometrial (LE) con un contenido en miRNAs específico que puede ser internalizado por el embrión para favorecer la adhesión embrionaria. OBJETIVOS: definir y caracterizar los diferentes tipos de vesículas extracelulares (VEs) secretados por el endometrio humano al LE y estudiar su contenido en DNA, determinar si es internalizado por el embrión y si afecta a su regulación energética. MATERIALES Y MÉTODOS: El aislamiento de las VEs del LE se realizó mediante centrifugación diferencial. Se estudió su morfología, marcadores moleculares, tamaño y concentración a lo largo del ciclo menstrual. Su cont…
Potential Roles of Muscle-Derived Extracellular Vesicles in Remodeling Cellular Microenvironment: Proposed Implications of the Exercise-Induced Myoki…
2021
Extracellular vesicles (EVs) have emerged as key players of intercellular communication and mediate crosstalk between tissues. Metastatic tumors release tumorigenic EVs, capable of pre-conditioning distal sites for organotropic metastasis. Growing evidence identifies muscle cell-derived EVs and myokines as potent mediators of cellular differentiation, proliferation, and metabolism. Muscle-derived EVs cargo myokines and other biological modulators like microRNAs, cytokines, chemokines, and prostaglandins hence, are likely to modulate the remodeling of niches in vital sites, such as liver and adipose tissues. Despite the scarcity of evidence to support a direct relationship between muscle-EVs…