Search results for "cryo-EM"

showing 3 items of 3 documents

Structural Studies Reveal that Endosomal Cations Promote Formation of Infectious Coxsackievirus A9 A-Particles, Facilitating RNA and VP4 Release

2022

Coxsackievirus A9 (CVA9), an enterovirus, is a common cause of pediatric aseptic meningitis and neonatal sepsis. During cell entry, enterovirus capsids undergo conformational changes leading to expansion, formation of large pores, externalization of VP1 N termini, and loss of the lipid factor from VP1. Factors such as receptor binding, heat, and acidic pH can trigger capsid expansion in some enteroviruses. Here, we show that fatty acid-free bovine serum albumin or neutral endosomal ionic conditions can independently prime CVA9 for expansion and genome release. Our results showed that CVA9 treatment with albumin or endosomal ions generated a heterogeneous population of virions, which could b…

11832 Microbiology and virologyalbumiinitviruksetImmunologyMicrobiologyendosomal ionic compositioncryoEMpicornavirusVirologyInsect Science1182 Biochemistry cell and molecular biologycryo-EMCryo-electron microscopyvirus structureA-particlealbumin
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Binding mode analysis of ABCA7 for the prediction of novel Alzheimer's disease therapeutics

2021

Graphical abstract

ATP Adenosine-triphosphateNBD nucleotide binding domainGSH reduced glutathionePolypharmacologyAlzheimer’s disease (AD)ATP-binding cassette transporterHTS high-throughput screeningBiochemistryABCA7Structural BiologyPLIF protein ligand interactionMSD membrane spanning domainPDB protein data bankTM transmembrane helixABC ATP-binding cassetteMultitarget modulation (PANABC)RMSD root mean square distanceABC transporter (ABCA1 ABCA4 ABCA7)Computer Science ApplicationsMOE Molecular Operating EnvironmentPharmacophoreSNP single-nucleotide polymorphismBiotechnologyResearch ArticleBBB blood-brain barrierBiophysicsDrug designComputational biologyBiologyAD Alzheimer’s diseasePET positron emission tomographyIC intracellular helixAPP amyloid precursor proteincryo-EM cryogenic-electron microscopyGeneticsHomology modelingBinding siteRational drug design and developmentComputingMethodologies_COMPUTERGRAPHICSNBD-cholesterol 7-nitro-2-13-benzoxadiazol-4-yl-cholesterolTransporterPSO particle swarm optimizationPET tracer (PETABC)ECD extracellular domainR-domain/region regulatory domain/regionABCA1biology.proteinEH extracellular helixTP248.13-248.65BODIPY-cholesterol 44-difluoro-4-bora-3a4a-diaza-s-indacene-cholesterolComputational and Structural Biotechnology Journal
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GRM2 tipa bakteriālo mikrokompartmentu daļiņu raksturojums

2020

Bakteriālie mikrokompartmenti (BMC) ir baktēriju organellas, kas iepako enzīmus proteīnu čaulā. Šī darba mērķis bija analizēt iespējas GRM2 tipa BMC iepakot heterologus enzīmus, kā arī apskatīt rekombinanti producētu BMC čaulas īpašības. Šajā darbā Klebsiella pneumoniae GRM2 tipa BMC čaulā tika testēta trīs Zymomonas mobilis etanolģenēzes enzīmu iepakošana kā starpnieku izmantojot natīvo CutC holīna liāzes enzīmu un SpyTag/SpyCatcher adhezīnu sistēmu. Veikta arī bakteriālo mikrokompartmentu čaulas analīze ar cryo-EM. Darba ietvaros veikts konstruktu dizains, proteīnu ekspresija, attīrīšana, un analīze ar TEM un cryo-EM. Rezultāti liecina par zemu iepakošanas efektivitāti, ja kā mediators iz…

Klebsiella pneumoniaebakteriālie mikrokompartmenticryo-EMbakteriālais metabolismsBioloģijabiotehnoloģija
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