Search results for "death"
showing 10 items of 1744 documents
Nivolumab Enhances In Vitro Effector Functions of PD-1+ T-Lymphocytes and Leishmania-Infected Human Myeloid Cells in a Host Cell-Dependent Manner
2017
Functional impairment of T-cells and a concomitant augmented expression of programmed death-1 (PD-1) have been observed in visceral leishmaniasis patients, as well as in experimental models for visceral and cutaneous leishmaniasis. The PD-1/PD-1-ligand (PD-1/PD-L) interaction negatively regulates T-cell effector functions, which are required for parasite control during leishmaniasis. The aim of this study was to elucidate the impact of the PD-1/PD-L axis in a human primary in vitro infection model of Leishmania major (Lm). Blocking the PD-1/PD-L interaction with nivolumab increased T-cell proliferation and release of the proinflammatory cytokines TNFα and IFNγ during the cocultivation of Lm…
Progenitor death drives retinal dysplasia and neuronal degeneration in a mouse model of Atrip-Seckel syndrome
2020
ABSTRACT Seckel syndrome is a type of microcephalic primordial dwarfism (MPD) that is characterized by growth retardation and neurodevelopmental defects, including reports of retinopathy. Mutations in key mediators of the replication stress response, the mutually dependent partners ATR and ATRIP, are among the known causes of Seckel syndrome. However, it remains unclear how their deficiency disrupts the development and function of the central nervous system (CNS). Here, we investigated the cellular and molecular consequences of ATRIP deficiency in different cell populations of the developing murine neural retina. We discovered that conditional inactivation of Atrip in photoreceptor neurons …
Increased autophagy and apoptosis contribute to muscle atrophy in a myotonic dystrophy type 1 Drosophila model
2015
ABSTRACT Muscle mass wasting is one of the most debilitating symptoms of myotonic dystrophy type 1 (DM1) disease, ultimately leading to immobility, respiratory defects, dysarthria, dysphagia and death in advanced stages of the disease. In order to study the molecular mechanisms leading to the degenerative loss of adult muscle tissue in DM1, we generated an inducible Drosophila model of expanded CTG trinucleotide repeat toxicity that resembles an adult-onset form of the disease. Heat-shock induced expression of 480 CUG repeats in adult flies resulted in a reduction in the area of the indirect flight muscles. In these model flies, reduction of muscle area was concomitant with increased apopto…
Original article Unfulfilled parenthood in the eyes of young adults
2014
Background The goal of the study was to present young adults’ perceptions of the experience of loss of a child at different stages of its prenatal development, as well as the level of declared support for couples after the loss. Participants and procedure The study used a scenario method based on the method by Claudia Lapman and Seana Dowling-Guyer (1995), translated into Polish by Joanna Szymańska (2013). One of the Berlin Social Support Scales (BSSS, Łyszczyńska, Kowalska, Mazurkiewicz, & Schwarzer, 2006) was also used. One hundred ninety two young adults (mean age = 26.76, SD = 4.64) took part in the research, mainly university students of technical subjects and humanities. Results The s…
Mediterranean diet impact on cardiovascular diseases: a narrative review
2017
Cardiovascular disease (CVD) accounts for more than 17 million deaths per year worldwide. It has been estimated that the influence of lifestyle on CVD mortality amounts to 13.7% for smoking, 13.2% for poor diet, and 12% for inactive lifestyle. These results deeply impact both the healthy status of individuals and their skills in working. The impact of CVD on productivity loss accounts for the 24% in total costs for CVD management. Mediterranean diet (MedD) can positively impact on natural history of CVD. It is characterized by a relatively high consumption of inexpensive and genuine food such as cereals, vegetables, legumes, nuts, fish, fresh fruits, and olive oil as the principal source of…
Toxicity of Branched-Chain Fatty Acids in Conjunctival Cell Line
2007
International audience; Purpose:: Tear film lipid composition is modified in patients suffering from chronic blepharitis. We previously found that branched chain fatty acids (BCFA) were significantly increased in tears of these patients, suggesting that BCFA may be involved in the disease. The toxicity of different BCFA as potent inducers of apoptosis has already been established in various cell lines but not in the conjunctiva. The aim of this work was to assess the toxic effects of BCFA in cultures of Chang conjunctival cells. Methods:: Chang’s human conjunctival cells were cultured during 4 or 24h with various concentrations of IsoC16 and IsoC20 as BCFA (from 50 to 200µM), by comparison …
Apoptotic-like Leishmania exploit the host´s autophagy machinery to reduce T-cell-mediated parasite elimination
2015
Apoptosis is a well-defined cellular process in which a cell dies, characterized by cell shrinkage and DNA fragmentation. In parasites like Leishmania, the process of apoptosis-like cell death has been described. Moreover upon infection, the apoptotic-like population is essential for disease development, in part by silencing host phagocytes. Nevertheless, the exact mechanism of how apoptosis in unicellular organisms may support infectivity remains unclear. Therefore we investigated the fate of apoptotic-like Leishmania parasites in human host macrophages. Our data showed--in contrast to viable parasites--that apoptotic-like parasites enter an LC3(+), autophagy-like compartment. The compartm…
Death and religion: from the old Tolstoy to the young Wittgenstein
2012
Mi propósito es doble. Mostrar cómo la pregunta por el sentido de la vida y la matriz del sentimiento religioso del Tolstoi maduro, que él considera cristianismo verdadero, se funda en la experiencia subjetiva del miedo ante la muerte y la angustia ante la soledad que la misma comporta. Este aspecto es el núcleo de su religiosidad por cuanto de él se deriva el valor de la fraternidad, centro de una moral humanista y altruista paradójica que sacraliza el vínculo amoroso entre los hombres a la par que se fundamenta en el sometimiento a la voluntad de Dios. Por otra parte, las concepciones de Tolstoi tal como se expresan no sólo en El Evangelio Abreviado, sino también en sus relatos literarios…
Succesio ab intestato: Concerning the death of Rodrigo de Mendoza, I Marquis of the Cenete
2017
La muerte de Rodrigo de Mendoza, I marqués del Cenete, sin testamento se vio envuelta de la incertidumbre propia del fallecimiento de un noble con un cuantioso patrimonio heredado en gran parte de su padre, el gran cardenal de España, sin especificar ni repartir y con tres hijas menores huérfanas sin un albacea designado por él. El gobernador general de Valencia, Lluís de Cabanyelles, del círculo más íntimo del marqués durante su estancia en la ciudad del Turia, junto a otros partícipes y testigos, impulsó el proceso de inventariado de todos sus bienes cuatro días después de su defunción, apelando, asimismo, al emperador Carlos V para que confirmase al virrey del reino de Valencia y hermano…
Targeting Orthosteric and Allosteric Pockets of Aromatase via Dual-Mode Novel Azole Inhibitors
2020
[Image: see text] Breast cancer (BC) is the most diffused cancer type in women and the second leading cause of death among the female population. Effective strategies to fight estrogen responsive (ER+) BC, which represents 70% of all BC cases, rely on estrogen deprivation, via the inhibition of the aromatase enzyme, or the modulation of its cognate estrogen receptor. Current clinical therapies significantly increased patient survival time. Nevertheless, the onset of resistance in metastatic BC patients undergoing prolonged treatments is becoming a current clinical challenge, urgently demanding to devise innovative strategies. In this context, here we designed, synthesized, and performed in …