Search results for "derived"
showing 10 items of 452 documents
SPARC is a new myeloid-derived suppressor cell marker licensing suppressive activities
2019
Myeloid-derived suppressor cells (MDSC) are well-known key negative regulators of the immune response during tumor growth, however scattered is the knowledge of their capacity to influence and adapt to the different tumor microenvironments and of the markers that identify those capacities. Here we show that the secreted protein acidic and rich in cysteine (SPARC) identifies in both human and mouse MDSC with immune suppressive capacity and pro-tumoral activities including the induction of epithelial-to-mesenchymal transition (EMT) and angiogenesis. In mice the genetic deletion of SPARC reduced MDSC immune suppression and reverted EMT. Sparc−/− MDSC were less suppressive overall and the granu…
Cholesterol Starvation and Hypoxia Activate the FVII Gene via the SREBP1-GILZ Pathway in Ovarian Cancer Cells to Produce Procoagulant Microvesicles
2019
AbstractInteraction between the transcription factors, hypoxia-inducible factor (HIF1α and HIF2α) and Sp1, mediates hypoxia-driven expression of FVII gene encoding coagulation factor VII (fVII) in ovarian clear cell carcinoma (CCC) cells. This mechanism is synergistically enhanced in response to serum starvation, a condition possibly associated with tumor hypoxia. This transcriptional response potentially results in venous thromboembolism, a common complication in cancer patients by producing procoagulant extracellular vesicles (EVs). However, which deficient serum factors are responsible for this characteristic transcriptional mechanism is unknown. Here, we report that cholesterol deficien…
Gametic embryogenesis through isolated microspore culture in mandarin (Citrus reticulata Blanco), Mandarino Tardivo Di Ciaculli: effect of meta-Topol…
2016
Haploid technology, allowing the single-step development of complete homozygous genotypes from heterozygous plants through gametic embryogenesis, has already an enormous impact on breeding programs of many important crops. Microspore embryogenesis can be carried out through in vitro culture of anther or isolated microspore. Experiments have been carried out via isolated microspore culture in Citrus reticulata (Blanco), Mandarino Tardivo di Ciaculli, investigating the influence of two culture media, of two plant growth regulator types and of two temperature treatments applied before the culture or just after the culture. After ten months of culture, for all the media tested, different struct…
Isolation and characterisation of tangerin-derived extracellular vesicles
2021
La Sicilia negli Orsi di Dino Buzzati e Lorenzo Mattotti
2022
Nella fiaba illustrata di Dino Buzzati La famosa invasione degli orsi in Sicilia (1945) e nella sua trasposizione filmica ad opera di Lorenzo Mattotti (2019) l’ambientazione siciliana è determinante. In entrambi i casi la Sicilia è un’isola con un grande vulcano – trasfigurata in un ambiente fantastico o riscritta secondo alcuni stereotipi – che i due autori utilizzano con accezioni diverse nella costituzione dell’ecosistema delle loro narrazioni. Soltanto attraverso una comparazione tra le due opere sarà possibile distinguere la rappresentazione delle due Sicilie che vengono raccontate con un intervallo di circa settanta anni. Se non si considera la storia degli Orsi soltanto una fiaba per…
INTRODUCING NON-ADHERENT PROGENITORS FROM ADIPOSE-DERIVED STEM CELLS (NAPADSCS): PROOF OF STEMNESS AND POSSIBLE FUTURE APPLICATIONS IN REGENERATIVE S…
2012
Introduction: Mesenchymal Stromal Cells (MSCs) can be isolated from adipose tissue and are currently used in vitro for experimental studies. MSCs are often mislabeled as Adipose-derived Stem Cells (ADSCs) despite presenting a more differentiated phenotype. The isolation and expansion of non-adherent progenitors from adipose-derived stem cells (napADSCs), has been achieved in our laboratory and is currently under investigation. In this study we investigate: a) the proof of stemness of such progenitors, and b) the feasibility of napADSCs adhesion over Integra® for cell colonization and for future differentiation and engineering of semi-synthetic tissues. Material and Methods: Adipose tissue (…
The associated sheaf functor theorem in algebraic set theory
2008
We prove a version of the associated sheaf functor theorem in Algebraic Set Theory. The proof is established working within a Heyting pretopos equipped with a system of small maps satisfying the axioms originally introduced by Joyal and Moerdijk. This result improves oil the existing developments by avoiding the assumption of additional axioms for small maps and the use of collection sites.
LabVIEW modeling and simulation, of the digital filters
2015
In order to study digital filters using virtual instrumentation a simulation program specifically designed for this purpose has been developed. To implement this program means to use the following facilities: studying digital Butterworth filters, Cebyshev, Bessel and Median and change their parameters: bandwidth, order, rank and slope; possibility of changing the input parameters: amplitude, offset and frequency; overlay over the input signal, to one of the types of noise such as white noise, Gaussian white noise and Poisson noise; choosing a type of digital filters: low pass, high pass, band-pass and band stop; waveforms graphical representation of the input and output signals; graphical r…
New combinations for mantle cell lymphoma: concerted action needed.
2011
Increased frequencies of CD11b+CD33+CD14+HLA-DRlowmyeloid-derived suppressor cells are an early event in melanoma patients
2014
Myeloid-derived suppressor cells (MDSC) are a heterogeneous cell population characterized by immunosuppressive activity. Elevated levels of MDSC in peripheral blood are found in inflammatory diseases as well as in malignant tumors where they are supposed to be major contributors to mechanisms of tumor-associated tolerance. We investigated the frequency and function of MDSC in peripheral blood of melanoma patients and observed an accumulation of CD11b(+) CD33(+) CD14(+) HLA-DR(low) MDSC in all stages of disease (I-IV), including early stage I patients. Disease progression and enhanced tumor burden did not result in a further increase in frequencies or change in phenotype of MDSC. By investig…