Search results for "ding"
showing 10 items of 16709 documents
Molecular Characterization of a Variant of Bacillus anthracis-Specific Phage AP50 with Improved Bacteriolytic Activity▿ †
2008
ABSTRACT The genome sequence of a Bacillus anthracis -specific clear plaque mutant phage, AP50c, contains 31 open reading frames spanning 14,398 bp, has two mutations compared to wild-type AP50t, and has a colinear genome architecture highly similar to that of gram-positive Tectiviridae phages. Spontaneous AP50c-resistant B. anthracis mutants exhibit a mucoid colony phenotype.
Immune responses during COVID-19 infection
2020
International audience; Over the past 16 years, three coronaviruses (CoVs), severe acute respiratory syndrome CoV (SARS-CoV) in 2002, Middle East respiratory syndrome CoV (MERS-CoV) in 2012 and 2015, and SARS-CoV-2 in 2020, have been causing severe and fatal human epidemics. The unpredictability of coronavirus disease-19 (COVID-19) poses a major burden on health care and economic systems across the world. This is caused by the paucity of in-depth knowledge of the risk factors for severe COVID-19, insufficient diagnostic tools for the detection of SARS-CoV-2, as well as the absence of specific and effective drug treatments. While protective humoral and cellular immune responses are usually m…
Molecular Basis of SARS-CoV-2 Nsp1-Induced Immune Translational Shutdown as Revealed by All-Atom Simulations.
2021
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic represents the most severe global health crisis in modern human history. One of the major SARS-CoV-2 virulence factors is nonstructural protein 1 (Nsp1), which, outcompeting with the binding of host mRNA to the human ribosome, triggers a translation shutdown of the host immune system. Here, microsecond-long all-atom simulations of the C-terminal portion of the SARS-CoV-2/SARS-CoV Nsp1 in complex with the 40S ribosome disclose that SARS-CoV-2 Nsp1 has evolved from its SARS-CoV ortholog to more effectively hijack the ribosome by undergoing a critical switch of Q/E158 and E/Q159 residues that perfects Nsp1's interactions…
Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme
1997
During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is hom…
Tumor targeting of baculovirus displaying a lymphatic homing peptide.
2008
Background Tumor-associated cells and vasculature express attractive molecular markers for site-specific vector targeting. To attain tumor-selective tropism, we recently developed a baculovirus vector displaying the lymphatic homing peptide LyP-1, originally identified by ex vivo/in vivo screening of phage display libraries, on the viral envelope by fusion to the transmembrane anchor of vesicular stomatitis virus G-protein. Methods In the present study, we explored the specificity and kinetics of viral binding and internalization as well as in vivo tumor homing of the LyP-1 displaying virus to elucidate the applicability of baculovirus for targeted therapies. Results We demonstrated that th…
The ALICE experiment at the CERN LHC
2008
Journal of Instrumentation 3(08), S08002 (2008). doi:10.1088/1748-0221/3/08/S08002
Fiksācijas disparitāte un konverģences nepietiekamība.
2020
Maģistra darbs ir uzrakstīts angļu valodā uz 47 lapām, tas satur 19 attēlus, 14 tabulas un 51 atsauci uz literatūras avotiem. Mērķis bija novērtēt fiksācijas disparitātes saistību ar konverģences nepietiekamību. Pētījumam bija divas daļas: 1. grupā bija 5 dalībnieki ar konverģences nepietiekamību, kas veica 12 nedēļu ilgu redzes terapiju, lai izvērtētu tās ietekmi uz binokulārajām funkcijām, lasīšanas ātrumu, CISS vērtējumu un fiksācijas disparitāti; 2. grupā bija 51 dalībnieks, kuri aizpildīja CISS aptauju un veica fiksācijas disparitātes mērījumus, lai izvērtētu šo divu parametru saistību. Redzes treniņi būtiski uzlaboju konverģence tuvuma punktu un pozitīvās fūzijas rezerves, kā arī sama…
(A,B) In vivo GCaMP6f signals recorded in layers M1, M5 and M9/10 of Mi1 (A) and Tm3 (B) neurons, before (blue, green) and after (gray, red) applicat…
2019
Sensory systems sequentially extract increasingly complex features. ON and OFF pathways, for example, encode increases or decreases of a stimulus from a common input. This ON/OFF pathway split is thought to occur at individual synaptic connections through a sign-inverting synapse in one of the pathways. Here, we show that ON selectivity is a multisynaptic process in the Drosophila visual system. A pharmacogenetics approach demonstrates that both glutamatergic inhibition through GluClα and GABAergic inhibition through Rdl mediate ON responses. Although neurons postsynaptic to the glutamatergic ON pathway input L1 lose all responses in GluClα mutants, they are resistant to a cell-type-specifi…
Pirms lasīšanas uzdevumi mācību grāmatu tekstiem vārdu krājuma paplašināšanai angļu valodas stundās tehnikumā
2019
Šis diplomdarbs pēta pirms lasīšanas uzdevumu ietekmi uz vārdu krājuma paplašināšanu un lasīšanas izpratni. Tā mērķis ir izstrādāt pirms lasīšanas papildu uzdevumus un izpētīt, kā tie veicina skolēnu sasniegumus vārdu krājuma uzlabošanā. Teorētiskā daļa ir vērsta uz teorijām par ciešu savstarpējo saistību starp lasīšanu un vārdu krājuma pārzināšanu. Empīriskā daļa apraksta gadījumu izpētes rezultātus. Datu vākšanas rīki parādīja priekšrocības, ko sniedz papildu pirms lasīšanas pasākumi studentu grupām, kas bija iesaistītas eksperimentā, salīdzinot ar studentiem, kuri izmantoja tikai mācību grāmatu materiālus. Tiek secināts, ka pirms lasīšanas uzdevumi ir paplašinājuši studentu vārdu krājumu…
Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging
2018
International audience; Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant binding proteins (OBPs) as sensing materials. Three rat OBP3 derivatives with customized binding properties were designed and immobilized on the same chip for the detection of VOCs in solution by surface plasmon resonance imaging (SPRi). We demonstrated that the proteins kept their binding properties after the immobilization under optimized conditions. The obtained olfactory biosensors exhibited very low limits of detection in both concentration (200pM of beta-ionone) and in molecular weight of …