Search results for "downy"

Changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves.

2011

International audience; The oomycete Plasmopara viticola is responsible for downy mildew, a severe grapevine disease. In infected grapevine leaves, we have observed an abnormal starch accumulation at the end of the dark period, suggesting modifications in starch metabolism. Therefore, several complementary approaches, including transcriptomic analyses, measurements of enzyme activities, and sugar quantification, were performed in order to investigate and to understand the effects of P. viticola infection on leaf starch and-to a larger extent-carbohydrate metabolism. Our results indicate that starch accumulation is associated with an increase in ADP-glucose pyrophosphorylase (AGPase) activit…

Are grapevine stomata involved in the elicitor-induced protection against downy mildew?

2009

Stomata, natural pores bordered by guard cells, regulate transpiration and gas exchanges between plant leaves and the atmosphere. These natural openings also constitute a way of penetration for microorganisms. In plants, the perception of potentially pathogenic microorganisms or elicitors of defense reactions induces a cascade of events, including H2O2 production, that allows the activation of defense genes, leading to defense reactions. Similar signaling events occur in guard cells in response to the perception of abscisic acid (ABA), leading to stomatal closure. Moreover, few elicitors were reported to induce stomatal closure in Arabidopsis and Vicia faba leaves. Because responses to ABA…

Living and Dead Aboveground Biomass in Mediterranean Forests: Evidence of Old-Growth Traits in a Quercus pubescens Willd. s.l. Stand

2017

For a long time, human impact has deeply simplified most of the forest ecosystems of the Mediterranean Basin. Here, forests have seldom had the chance to naturally develop a complex and multilayered structure, to host large and old trees and rich biological communities, approaching old-growth conditions. Also for this reason, limited information is currently available about Mediterranean old-growth forests, particularly with regard to deadwood. The main aim of this work is to help fill this critical knowledge gap. In Sicily (Italy), we identified a Quercus pubescens forest that seemed to show some typical old-growth features. Total living volume (360 m3 ha−1) and basal area (34 m2 ha−1) wer…

Image analysis methods for assessment of H2O2 production and Plasmopara viticola development in grapevine leaves: application to the evaluation of re…

2013

article i nfo The grapevine downy mildew (Plasmopara viticola) provokes severe damages and destroys the harvest in the absence of an effective protection. Numerous fungicide treatments are thus generally necessary. To promote a sustainable production, alternative strategies of protection including new antifungal molecules, resistant geno- types or elicitor-induced resistance are under trial. To evaluate the relevance of these strategies, resistance tests are required. In this context, three image analysis methods were developed to read the results of tests performed to assessP.viticolasporulation and mycelial development, and H 2 O 2 production in leaves. They have been validated using elic…

Stomatal deregulation in Plasmopara viticola-infected grapevine leaves.

2007

International audience; In grapevine, the penetration and sporulation of Plasmopara viticola occur via stomata, suggesting functional relationships between guard cells and the pathogen. This assumption was supported by our first observation that grapevine (Vitis vinifera cv. Marselan) cuttings infected by P. viticola wilted more rapidly than healthy ones when submitted to water starvation. • Here, complementary approaches measuring stomatal conductance and infrared thermographic and microscopic observations were used to investigate stomatal opening/closure in response to infection. • In infected leaves, stomata remained open in darkness and during water stress, leading to increased transpir…

Influence of leaf age on induced resistance in grapevine against Plasmopara viticola

2012

International audience; Sulfated laminarin (PS3) has previously been shown to induce resistance of grapevine leaves against the oomycete Plasmopara viticola, the causal agent of grape downy mildew. Here, we observed that the level of PS3-induced resistance (PS3-IR) was higher in the adult leaf (in position P3) than in the younger, not fully expanded leaf (in position P1, located above P3). By investigating grapevine defense reactions upon PS3 treatment and inoculation, we found that the production of H2O2, of phytoalexins, and the deposition of phenolics were more abundant in P3 than in P1 leaves. In addition, PS3 significantly reduced stomatal colonization by zoospores only in P3 leaves. T…

Soybean and casein hydrolysates induce grapevine immune responses and resistance against Plasmopara viticola

2014

International audience; Plasmopara viticola, the causal agent of grapevine downy mildew, is one of the most devastating grape pathogen in Europe and North America. Although phytochemicals are used to control pathogen infections, the appearance of resistant strains and the concern for possible adverse effects on environment and human health are increasing the search for alternative strategies. In the present investigation, we successfully tested two protein hydrolysates from soybean (soy) and casein (cas) to trigger grapevine resistance against P. viticola. On Vitis vinifera cv. Marselan plants, the application of soy and cas reduced the infected leaf surface by 76 and 63%, as compared to th…

2017

Induction of plant resistance against pathogens by defense elicitors constitutes an attractive strategy to reduce the use of fungicides in crop protection. However, all elicitors do not systematically confer protection against pathogens. Elicitor-induced resistance (IR) thus merits to be further characterized in order to understand what makes an elicitor efficient. In this study, the oligosaccharidic defense elicitors H13 and PS3, respectively, ineffective and effective to trigger resistance of grapevine leaves against downy mildew, were used to compare their effect on the global leaf metabolism. Ultra high resolution mass spectrometry (FT-ICR-MS) analysis allowed us to obtain and compare t…

An ethoxylated surfactant enhances the penetration of the sulfated laminarin through leaf cuticle and stomata, leading to increased induced resistanc…

2016

International audience; Some β-1,3-glucans and particularly sulfated laminarin (PS3) are known as resistance inducers (RIs) in grapevine against the downy mildew. However, their efficacy in vineyard is still often too low, which might be caused by a limited penetration through the leaf cuticle following spray application. We used (14) C-sucrose uptake experiments with grapevine leaves in order to select a surfactant as saccharide penetration enhancer. Our results showed that though sucrose foliar uptake was low, it was strongly enhanced by Dehscofix CO125 (DE), a highly ethoxylated surfactant. Fluorescent saccharides were then produced and laser scanning microscopy was used to analyze their…

Downy-oak woods of Italy: phytogeographical remarks on a controversial taxonomic and ecologic issue

2014

The importance of downy oak as an integral component of the "submediterranean" woods has been underscored by many studies. Nevertheless, terms like "submediterranean" and "downy oak" are some of the most poorly understood concepts in European phytogeographic and taxonomic research. Downy oak is well known to be a problematic taxon. The name "Quercus pubescens" (= Q. humilis) combines populations characterized by increasing phenotypic and genomic polymorphisms along north-south gradients, which is explained as the result of a "founder effect" produced by a relatively fast post-glacial re-colonization of the northern areas through rare long-distance dispersal events. On the other hand, polymo…