Search results for "droplet"

Comparing encapsulation-dehydration and droplet-vitrification for cryopreservation of sugarcane (Saccharum spp.) shoot tips

2011

Abstract In this study, in vitro shoot tips of two sugarcane clones were successfully cryopreserved using encapsulation-dehydration and droplet-vitrification with two vitrification solutions, PVS2 and PVS3. For both clones, encapsulation-dehydration induced significantly higher recovery, reaching 60% for clone H70-144 and 53% for clone CP68-1026, compared with droplet-vitrification in which recovery was 33–37% for clone H70-144 and 20–27% for clone CP68-1026. Optimal conditions included preculture of encapsulated shoot apices for 24 h in liquid medium with 0.75 M sucrose and dehydration with silica gel to 20% moisture content (fresh weight basis) before direct immersion in liquid nitrogen. …

Analysis of Dissolved-Gas Atomization: Supercritical CO2 Dissolved in Water

2010

Supercritical dissolved-gas atomization is an atomization process in which carbon dioxide at temperature and pressure above its critical point is used as the atomizing gas. The spray characteristics in terms of droplets size and distribution have been experimentally studied using a laser diffraction method based on a Malvern apparatus. The main parameter that influences the droplets size is the gas-to-liquid mass ratio (GLR); the injection pressure in the range of 7.4-13 MPa has a minor effect. Upon variation of the GLR from 0.5 to 3, the droplet mean diameter changes from about 8.0 to 2.0 μm; very narrow droplet size distributions are also produced. From the point of view of the atomizatio…

Magnētiskā šķidruma pilieni rotējošā laukā: teorija, eksperimenti un simulācijas

2022

Magnētiskā šķidruma pilieni ir reizē deformējami un ietekmējami ar ārējo magnētisko lauku, kas tos padara par interesantu materiālu, kas is atradis daudzus pielietojumus mikrofluīdikā. Šajā darbā tiek aplūkota šādu pilienu dinamika rotējošā magnētiskajā laukā. Pilieni tiek pētīti, izmantojot vairākas pieejas - teorētiski, eksperimentāli un izmantojot simulācijas. Kad rotējošais magnētiskais lauks lauks ir vājš un piliena deformācija ir maza, piliena kustība tiek aprēķināta analītiski. Konstatēts, ka piliena formas attīstību laikā regulē trīs nelineāru diferenciālvienādojumu sistēma. Mazo deformāciju tuvinājumā piliena uzvedību kvalitatīvi raksturo viens parametrs, kas ir proporcionāls kapil…

Droplet-to-droplet microarray for drug screening in picoliter scale

2012

The serosa of Manduca sexta (Insecta, Lepidoptera): ontogeny, secretory activity, structural changes, and functional considerations

2001

In Manduca sexta, the blastoderm forms successively and becomes immediately cellularized as the cleavage energids reach the surface of the oocyte. Presumptive serosal cells are large and contain 2 or 4 large polyploid nuclei; presumptive embryonic cells are small and mononuclear. All parts of the blastoderm participate in the uptake and digestion of yolk material. About 10 h post-oviposition, the blastoderm breaks at the amnioserosal fold and the extraembryonic part closes above the germ band and constitutes the serosa (12 h post-oviposition, i.e. 10% development completed). At once, the serosa starts to secrete a cuticle consisting of an epi- and a lamellated endocuticle. Detachment of the…

Ultrastructure of the sternal glands inThrips validus uzel (Thysanoptera, terebrantia)

1978

The ultrastructure of the sternal glands of the males ofThrips validus is consistent with the hypothesis that they are involved in pheromone production. The secretory cells, which are strongly modified epidermal cells, are characterized by the abundance of mitochondria and the presence of agranular, tubular endoplasmic reticulum (ATER) and microbodies. Each of the numerous apical microvilli encloses one or several tubular extensions of ATER. Some mitochondria are in close contact with ATER tubules. The mitochondrial matrix is probably involved in the elaboration of the secretory product, which is extruded and possibly modified by the agranular reticulum. The secretion is sudanophilic, but n…

Chironomus riparius exposure to fullerene-contaminated sediment results in oxidative stress and may impact life cycle parameters

2015

A key component of understanding the potential environmental risks of fullerenes (C60) is their potential effects on benthic invertebrates. Using the sediment dwelling invertebrate Chironomus riparius we explored the effects of acute (12 h and 24 h) and chronic (10 d, 15 d, and 28 d) exposures of sediment associated fullerenes. The aims of this study were to assess the impact of exposure to C60 in the sediment top layer ((0.025, 0.18 and 0.48) C60 mg/cm2) on larval growth, oxidative stress and emergence rates and to quantify larval body burdens in similarly exposed organisms. Oxidative stress localization was observed in the tissues next to the microvilli and exoskeleton through a method fo…

Active mixing inside double emulsion segments in continuous flow

2015

Fast mixing is essential for many microfluidic applications, especially for flow at low Reynolds numbers. A capillary tube-in-tube coaxial flow setup in combination with a glass microreactor was used to produce immiscible multiphase segments. These double emulsion segments are composed of an organic solvent as the shell (outer) phase and a completely fluorinated liquid (Fluorinert® FC-40) as the core (inner) phase. Due to the higher density of the core droplets, they are responsive to changing their position to the force of gravity (g-force). By gently shaking or jiggling the reactor, the core drop flows very fast in the direction of the g-field without leaving the shell organic phase segme…

RAB3GAP1 and RAB3GAP2 modulate basal and rapamycin-induced autophagy

2014

Macroautophagy is a degradative pathway that sequesters and transports cytosolic cargo in autophagosomes to lysosomes, and its deterioration affects intracellular proteostasis. Membrane dynamics accompanying autophagy are mostly elusive and depend on trafficking processes. RAB GTPase activating proteins (RABGAPs) are important factors for the coordination of cellular vesicle transport systems, and several TBC (TRE2-BUB2-CDC16) domain-containing RABGAPs are associated with autophagy. Employing C. elegans and human primary fibroblasts, we show that RAB3GAP1 and RAB3GAP2, which are components of the TBC domain-free RAB3GAP complex, influence protein aggregation and affect autophagy at basal an…

Smoothed particles hydrodynamics numerical simulations of droplets walking on viscous vibrating liquid

2016

We study the phenomenon of the "walking droplet", by means of numerical fluid dynamics simulations using the Smoothed Particle Hydrodynamics numerical method. This phenomenon occurs when a millimetric drop is released on the surface of an oil of the same composition, contained in a tank and subjected to vertical oscillations of frequency and amplitude very close to the Faraday instability threshold. At appropriate values of the parameters of the system under study, the oil droplet jumps permanently on the surface of the vibrating liquid forming a localized wave-particle system, reminding the behaviour of a wave particle quantum system as suggested by de Broglie. In our study, we made releva…