Search results for "echinoderm"

showing 10 items of 85 documents

Humoral responses during wound healing in Holothuria tubulosa (Gmelin, 1788)

2021

Abstract Wounds in living organisms trigger tissue-repair mechanisms. The sea cucumber (Holoturia tubulosa) is an excellent model species for achieving a better understanding of the humoral and cellular aspects involved in such healing processes. Consequently, this study assesses data on its morphometric, physiological and humoral responses 1, 2, 6, 24 and 48h after wound induction. In particular, morphometric data on the weight, width, length and coelomic-fluid volume of the species were estimated at different times during our experiments. In addition, the humoral aspects related to the enzymatic activity of esterase, alkaline phosphatase and peroxidase, as well as the cytotoxic activity o…

PhysiologySettore BIO/05 - ZoologiaBiochemistryEsteraseCytotoxic activity Echinoderm Enzyme activity Regeneration mechanism Immunity sea cucumber03 medical and health sciencesSea cucumberAnimalsHolothuriaCytotoxic T cellSettore BIO/06 - Anatomia Comparata E CitologiaMolecular Biology030304 developmental biologyWound Healing0303 health sciencesSheepbiologyBody WeightHolothuria tubulosa04 agricultural and veterinary sciencesbiology.organism_classificationEnzyme assayFISIOLOGIABiochemistry040102 fisheriesbiology.protein0401 agriculture forestry and fisheriesAlkaline phosphataseRabbitssense organsWound healingPeroxidaseComparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
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Cell cooperation in coelomocyte cytotoxic activity of Paracentrotus lividus coelomocytes

2007

The coelomic fluid from the sea urchin Paracentrotus lividus contains several coelomocyte types including amoebocytes and uncoloured spherulocytes involved in immune defences. In the present paper, we show a Ca(2+)-dependent cytotoxic activity for the unfractionated coelomocytes assayed in vitro, with rabbit erythrocytes and the K562 tumour cell line. In a plaque-forming assay, whole coelomocyte preparations as well as density gradient separated coelomocyte populations revealed that cell populations enriched in uncoloured spherulocytes, exerted high cytotoxic activity by releasing lysins in the presence of amoebocytes. This cooperative effect could be dependent on soluble factors released b…

Programmed cell deathErythrocytesPhysiologyCytotoxicityCell CommunicationCell SeparationBiochemistryParacentrotus lividusbiology.animalCentrifugation Density GradientAnimalsHumansCytotoxic T cellCytotoxicityMolecular BiologySea urchinCoelomocyteCoelomocyte cooperationInnate immunityCell DeathEchinodermbiologyAnatomybiology.organism_classificationIn vitroCell biologyParacentrotus lividusCell cultureParacentrotusRabbitsCoelomocyteK562 CellsComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
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A Survey on Tubulin and Arginine Methyltransferase Families Sheds Light on

2019

Tubulins and microtubules (MTs) represent targets for taxane-based chemotherapy. To date, several lines of evidence suggest that effectiveness of compounds binding tubulin often relies on different post-translational modifications on tubulins. Among them, methylation was recently associated to drug resistance mechanisms impairing taxanes binding. The sea urchin is recognized as a research model in several fields including fertilization, embryo development and toxicology. To date, some α- and β-tubulin genes have been identified in P. lividus, while no data are available in echinoderms for arginine methyl transferases (PRMT). To evaluate the exploiting of the sea urchin embryo in the field o…

Protein-Arginine N-MethyltransferasesEmbryo NonmammalianPRMTechinodermsIntracellular Signaling Peptides and Proteinsmacromolecular substancesCytostatic AgentsMethylationTubulin ModulatorsArticlearginine methylationsea urchintubulinpost-translational modificationSea Urchinsembryonic structuresToxicity TestsAnimalsProtein Processing Post-TranslationalInternational journal of molecular sciences
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Changes in the proteome of sea urchin Paracentrotus lividus coelomocytes in response to LPS injection into the body cavity.

2020

Background The immune system of echinoderm sea urchins is characterised by a high degree of complexity that is not completely understood. The Mediterranean sea urchin Paracentrotus lividus coelomocytes mediate immune responses through phagocytosis, encapsulation of non-self particles, and production of diffusible factors including antimicrobial molecules. Details of these processes, and molecular pathways driving these mechanisms, are still to be fully elucidated. Principal findings In the present study we treated the sea urchin P. lividus with the bacterial lipopolysaccharide (LPS) and collected coelomocytes at different time-points (1, 3, 6 and 24 hours). We have shown, using label-free q…

ProteomicsLipopolysaccharidesProteomeHydrolasesBiochemistry0302 clinical medicineParacentrotusMedicine and Health SciencesSea urchinCoelomocyteImmune ResponseCytoskeleton0303 health sciencesPhagocytesMultidisciplinarybiologyChemistryQREukaryotaAnimal ModelsCell biologyEnzymesEchinodermExperimental Organism Systems030220 oncology & carcinogenesisProteomeParacentrotusMedicineProtein Interaction NetworksCellular Structures and OrganellesNetwork AnalysisResearch ArticleEchinodermsComputer and Information Sciencesfood.ingredientScienceImmunologyLipopolysaccharideEndocytosisResearch and Analysis MethodsParacentrotus lividusLymphatic System03 medical and health sciencesfoodPhagocytosisbiology.animalHeat shock proteinDNA-binding proteinsAnimalsProtein Interactions030304 developmental biologyPhagocytosiAnimalOrganismsBiology and Life SciencesProteinsCell Biologybiology.organism_classificationInvertebratesCytoskeletal ProteinsGuanosine TriphosphataseProtein-Protein InteractionsPhagocyteImmune SystemSea UrchinsAnimal StudiesEnzymologyParacentrotuPLoS ONE
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A survey on tubulin and arginine methyltransferase families sheds light on p. lividus embryo as model system for antiproliferative drug development

2019

Tubulins and microtubules (MTs) represent targets for taxane-based chemotherapy. To date, several lines of evidence suggest that effectiveness of compounds binding tubulin often relies on different post-translational modifications on tubulins. Among them, methylation was recently associated to drug resistance mechanisms impairing taxanes binding. The sea urchin is recognized as a research model in several fields including fertilization, embryo development and toxicology. To date, some &alpha

Sea urchinPRMTSettore BIO/11 - Biologia MolecolareDrug actionmacromolecular substancesBiologyCatalysisCatalysilcsh:ChemistryInorganic ChemistryMicrotubuleArginine methylationTubulinbiology.animalGene familyPhysical and Theoretical ChemistrySea urchinlcsh:QH301-705.5Molecular BiologySpectroscopyEchinodermechinodermsOrganic ChemistryEmbryoComputer Science Applications1707 Computer Vision and Pattern RecognitionGeneral MedicineMethylationComputer Science ApplicationsCell biologyTubulinDrug developmentlcsh:Biology (General)lcsh:QD1-999embryonic structuresbiology.proteinPost-translational modification
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Are control of extracellular acid-base balance and regulation of skeleton genes linked to resistance to ocean acidification in adult sea urchins?

2020

SCOPUS: ar.j

Settore BIO/07 - EcologiaCO2 ventsEnvironmental EngineeringClimate ChangeOceans and SeasMechanical propertiesAcid–base homeostasisEnvironnement et pollutionDICGene expressionExtracellularEnvironmental ChemistryAnimalsSeawaterWaste Management and DisposalGeneSkeletonCO2 ventAcid-Base EquilibriumResistance (ecology)ChemistryOcean acidificationOcean acidificationCarbon DioxideHydrogen-Ion ConcentrationbiomineralizationPollutionSkeleton (computer programming)adult sea urchinsCell biologyTechnologie de l'environnement contrôle de la pollutionqPCRSea UrchinsTraitement des déchetsGene expressionEchinodermsThe Science of the total environment
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Are homalozoans echinoderms? An answer from the extraxial-axial theory

2000

Homalozoans include four classes of non-pentamerous Paleozoic echinoderms: Homostelea (cinctans), Ctenocystoidea (ctenoid-bearing homalozoans), Homoiostelea (solutes), and Stylophora (cornutes and mitrates). Their atypical morphologies have historically made it difficult to relate them to other classes. Therefore, their systematic positions have been represented by two hypotheses (H): as stem taxa to echinoderms (H1) or as stem taxa to chordates (H2). These conclusions rest on previous inability to recognize synapomorphies with more crownward echinoderms, resulting in a forcing of the homalozoans down the phylogenetic tree that is more artifactual than evolutionary. The Extraxial-Axial Theo…

SynapomorphyEcologybiologyPhylogenetic treePhylumEcologyPaleontologyStylophorabiology.organism_classificationTaxonEchinodermEvolutionary biologyPolyphylyGeneral Agricultural and Biological SciencesCladeEcology Evolution Behavior and SystematicsPaleobiology
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New specimens of Lingulocystis Thoral, 1935 (Eocrinoidea, Blastozoa) from the Arenig (Lower Ordovician) of Montagne noire (southern France): Intraspe…

2007

16 pages; The genus Lingulocystis Thoral, 1935 is a preponderant component of the echinoderm fauna of the Lower Ordovician of Montagne noire (France), as suggested by its abundant remains. Its morphology is characterized by a flattened theca, composed of a frame enclosing numerous small polygonal central plates. The new specimens have been determined partly as Lingulocystis elongata Thoral, 1935 and partly as Lingulocystis aff. deani Ubaghs, 1994. This study is the first report of Lingulocystis in the Foulon Formation. New stratigraphic ranges have been defined for both species: L. elongata from the early Tremadoc (Saint- Chinian Formation) to the middle Arenig (lower part of the Landeyran …

Systematics010506 paleontologyTaphonomybiologyFaunaPalaeoecologyPaleontology010502 geochemistry & geophysicsbiology.organism_classification01 natural sciencesBlastozoaPaleontologyEchinodermGenusSystematicsPaleoecologyFrance[SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/PaleontologyEocrinoideaLower OrdovicianGeologyEchinodermata[ SDU.STU.PG ] Sciences of the Universe [physics]/Earth Sciences/Paleontology0105 earth and related environmental sciencesAnnales de Paléontologie
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Description of two new fossil echinoids (Echinodermata: Echinoidea) from the Early Hauterivian (Early Cretaceous) of the Paris Basin (France)

2012

International audience; Deux nouveaux genres et espèces d'échinides, Salvaster roberti gen. et sp. nov. et Pygolampas edita gen. et sp. nov. provenant de la Formation des Calcaires à Spatangues (CSF) sont décrits. La CSF correspond à des sédiments calcaréo-argileux déposés dans le sud-est du bassin de Paris (France) à l'Hauterivien inférieur (Crétacé inférieur). Ces dépôts sont datés de la chronozone à Acanthodiscus radiatus, un intervalle de temps qui a correspondu à un haut niveau marin général en Europe de l'Ouest. La CSF a pourtant livré une riche faune fossile d'environnement marin peu profond, principalement représentée par des invertébrés benthiques. Parmi ceux-ci, 54 espèces d'échin…

Systematics010506 paleontologynouveau genrenouvelle espèceFaunaCassiduloida[SDU.STU]Sciences of the Universe [physics]/Earth Sciences010502 geochemistry & geophysics01 natural sciencesPaleontologysystématiqueAnimaliaEcology Evolution Behavior and SystematicsSea level0105 earth and related environmental sciencesInvertebrateHauterivienTaxonomyHolasteroidabiologyEcology[ SDU.STU ] Sciences of the Universe [physics]/Earth SciencesEchinoideaBiodiversitybiology.organism_classificationCretaceousNotAcanthodiscus[SDU.STU] Sciences of the Universe [physics]/Earth SciencesAnimal Science and ZoologyChronozoneCassiduloidaEchinodermata
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Coelomocytes and post-traumatic response in the common sea star Asterias rubens.

2008

Coelomocytes are recognized as the main cellular component of the echinoderm immune system. They are the first line of defense and their number and type can vary dramatically during infections or following injury. Sea stars have been used as a model system to study the regeneration process after autotomy or predation. In the present study we examined the cellular and biochemical responses of coelomocytes from the European sea star Asterias rubens to traumatic stress using immunochemical and biochemical approaches. In terms of trauma and post-traumatic stress period, here we consider the experimental arm amputation and the repair phase involved in the first 24 hours post-amputation, which mi…

Time FactorsImmunocytochemistryPopulationCell CountBiochemistryAndrologymedicineAnimalsHSP70 Heat-Shock ProteinsPseudopodiaeducationGlycoproteinseducation.field_of_studyPhagocytesbiologyAsteriasAntibodies MonoclonalCell BiologyOriginal Articlesbiology.organism_classificationStrongylocentrotus purpuratusCoelomic epitheliummedicine.anatomical_structureEchinodermAsteriasImmunologyCoelomAutotomyCell stresschaperones
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