Search results for "ekspressio"
showing 10 items of 40 documents
The effects of short-term glyphosate-based herbicide exposure on insect gene expression profiles
2023
Glyphosate-based herbicides (GBHs) are the most frequently used herbicides worldwide. The use of GBHs is intended to tackle weeds, but GBHs have been shown to affect the life-history traits and antioxidant defense system of invertebrates found in agroecosystems. Thus far, the effects of GBHs on detoxification pathways among invertebrates have not been sufficiently investigated. We performed two different experiments—1) the direct pure glyphosate and GBH treatment, and 2) the indirect GBH experiment via food—to examine the possible effects of environmentally relevant GBH levels on the survival of the Colorado potato beetle (Leptinotarsa decemlineata) and the expression profiles of their deto…
Methylation status of VTRNA2-1/nc886 is stable across populations, monozygotic twin pairs and in majority of tissues.
2022
Aims & methods: The aim of this study was to characterize the methylation level of a polymorphically imprinted gene, VTRNA2-1/nc886, in human populations and somatic tissues.48 datasets, consisting of more than 30 tissues and >30,000 individuals, were used. Results: nc886 methylation status is associated with twin status and ethnic background, but the variation between populations is limited. Monozygotic twin pairs present concordant methylation, whereas similar to 30% of dizygotic twin pairs present discordant methylation in the nc886 locus. The methylation levels of nc886 are uniform across somatic tissues, except in cerebellum and skeletal muscle. Conclusion: The nc886 imprint may be est…
Long-term leisure-time physical activity vs. inactivity, physical fitness, body composition and metabolic health characteristics : a co-twin control …
2013
Exercise-induced expression of angiogenic growth factors in skeletal muscle and in capillaries of healthy and diabetic mice
2008
Background. Diabetes has negative, and exercise training positive, effects on the skeletal muscle vasculature, but the mechanisms are not yet fully understood. In the present experiment the effects of running exercise on the mRNA expression of pro- and antiangiogenic factors were studied in healthy and diabetic skeletal muscle. The responses in capillaries and muscle fibers, collected from the muscle with laser capture microdissection, were also studied separately. Methods. Healthy and streptozotocin-induced diabetic mice were divided into sedentary and exercise groups. Exercise was a single bout of 1 h running on a treadmill. Gastrocnemius muscles were harvested 3 h and 6 h post exercise, …
Effects of bioheapleaching technology utilizing metal mine emissions on fish in boreal freshwaters
2018
Emissions from metal mining activities are known to deteriorate the quality of aquatic habitats and impair the condition and reproductive potential of fish. Metal extraction by biomining methods has been considered to cause fewer emissions, but for example in Finland, the impacts of a bioheapleaching technology utilizing metal mine on the local freshwaters have been substantial. In this thesis, the impacts of the bioheapleaching mine emissions on three native fish species, brown trout (Salmo trutta) European perch (Perca fluviatilis) and whitefish (Coregonus lavaretus pallasi), were investigated. Manganese and sulphate concentrations have been elevated in the mining impacted waters, and in …
Gene expression in TGFbeta-induced epithelial cell differentiation in a three-dimensional intestinal epithelial cell differentiation model
2006
Abstract Background The TGFβ1-induced signal transduction processes involved in growth and differentiation are only partly known. The three-dimensional epithelial differentiation model, in which T84 epithelial cells are induced to differentiate either with TGFβ1 or IMR-90 mesenchymal cell-secreted soluble factors, is previously shown to model epithelial cell differentiation seen in intestine. That model has not been used for large scale gene expression studies, such as microarray method. Therefore the gene expression changes were studied in undifferentiated and differentiated three-dimensional T84 cultures with cDNA microarray method in order to study the molecular changes and find new play…
Supplementary data to: Sequence and Regulatory Variation in Acetylcholinesterase Genes Contribute to Insecticide Resistance in Different populations …
2021
This is data used in Aigi Margus, Saija Piiroinen, Philipp Lehmann, et al. Sequence and Regulatory Variation in Acetylcholinesterase Genes Contribute to Insecticide Resistance in Different populations of Leptinotarsa decemlineata. Authorea. September 26, 2021. Data contains bioassay data, enzyme activity data, gene expression data and gene sequence data used to research insect herbivores resistance to insecticides. See the attached data description file for more information.
Variants associated with HHIP expression have sex-differential effects on lung function [version 1; peer review: 2 approved]
2020
Background: Lung function is highly heritable and differs between the sexes throughout life. However, little is known about sex-differential genetic effects on lung function. We aimed to conduct the first genome-wide genotype-by-sex interaction study on lung function to identify genetic effects that differ between males and females. Methods: We tested for interactions between 7,745,864 variants and sex on spirometry-based measures of lung function in UK Biobank (N=303,612), and sought replication in 75,696 independent individuals from the SpiroMeta consortium. Results: Five independent single-nucleotide polymorphisms (SNPs) showed genome-wide significant (P<5x10-8) interactions with sex on …
Global gene expression profiles in skeletal muscle of monozygotic female twins discordant for hormone replacement therapy
2010
Aging is accompanied by inexorable loss of muscle tissue. One of the underlying causes for this is the massive change in the hormonal milieu of the body. The role of a female sex steroid – estrogen – in these processes is frequently neglected, although the rapid decline in its production coincides with a steep deterioration in muscle performance. We recruited 54- to 62-year-old monozygotic female twin pairs discordant for postmenopausal hormone replacement therapy (HRT, n = 11 pairs; HRT use 7.3 ± 3.7 years) from the Finnish Twin Cohort to investigate the association of long-term, estrogen-based HRT with skeletal muscle transcriptome. Pathway analysis of muscle transcript profiles revealed …
Luurankolihaksen geeniekspressio ja perinnöllinen kestävyyssuorituskyky
2007
Pullinen, Katri 2007. Luurankolihaksen geeniekspressio ja perinnöllinen kestä-vyyssuorituskyky. Liikuntabiologian laitos, Jyväskylän yliopisto. Liikuntafysiolo-gian Kandidaatin tutkielma, 49 s. Perinnöllistä kestävyyssuorituskykyä ja kestävyysharjoittelun aiheuttamia adap-taatioita voidaan tutkia geeniekspressiotasolla tekemällä microarray-analyysi. Kestä-vyysharjoittelun aiheuttamia adaptaatioita tutkittaessa on havaittu muun muassa mito-kondrioiden biogeneesiin ja metaboliaan liittyvien geenien ilmenemisen kasvua. Kestä-vyyssuorituskyvyn perinnöllisyyttä ei ole tutkittu geeniekspressiotasolla ihmisillä eikä eläimillä. Ihmisillä se on erittäin hankalaa, joten sitä varten voidaan luoda eläi…