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showing 10 items of 33096 documents

RNA uridylation and decay in plants

2018

RNA uridylation consists of the untemplated addition of uridines at the 3′ extremity of an RNA molecule. RNA uridylation is catalysed by terminal uridylyltransferases (TUTases), which form a subgroup of the terminal nucleotidyltransferase family, to which poly(A) polymerases also belong. The key role of RNA uridylation is to regulate RNA degradation in a variety of eukaryotes, including fission yeast, plants and animals. In plants, RNA uridylation has been mostly studied in two model species, the green algae Chlamydomonas reinhardtii and the flowering plant Arabidopsis thaliana . Plant TUTases target a variety of RNA substrates, differing in size and function. These RNA substrates include …

0106 biological sciences0301 basic medicineSmall interfering RNATerminal nucleotidyltransferaseRNA StabilitymRNAArabidopsisChlamydomonas reinhardtiiUridylationBiology01 natural sciencesRNA decayGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesRNA degradationSettore AGR/07 - Genetica AgrariamicroRNAGene silencing[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyUridineComputingMilieux_MISCELLANEOUSPolymerase2. Zero hungerMessenger RNABiochemistry Genetics and Molecular Biology (all)fungiRNAfood and beverages[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biologyArticlesPlantsRibosomal RNAbiology.organism_classificationCell biology030104 developmental biologyAgricultural and Biological Sciences (all)biology.proteinRNARNA InterferenceGeneral Agricultural and Biological SciencesChlamydomonas reinhardtii010606 plant biology & botany
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Infestation of broad bean (Vicia faba) by the green stink bug (Nezara viridula) decreases shoot abscisic acid contents under well-watered and drought…

2017

The response of broad bean (Vicia faba) plants to water stress alone and in combination with green stink bug (Nezara viridula) infestation was investigated through measurement of: (1) leaf gas exchange; (2) plant hormone titres of abscisic acid (ABA) and its metabolites, and of salicylic acid (SA); and (3) hydrogen peroxide (H2O2) content. Furthermore, we evaluated the effects of experimentally water-stressed broad-bean plants on N. viridula performance in terms of adult host–plant preference, and nymph growth and survival. Water stress significantly reduced both photosynthesis (A) and stomatal conductance (gs), while infestation by the green stink bug had no effects on photosynthesis but s…

0106 biological sciences0301 basic medicineStomatal conductanceGreen stink bugsalicylic acidhydrogen peroxidePlant Sciencelcsh:Plant culturemedicine.disease_cause01 natural sciences03 medical and health scienceschemistry.chemical_compoundPhotosynthesiInfestationparasitic diseasesmedicinelcsh:SB1-1110Abscisic acidOriginal Researchwater deficitphotosynthesisbiologyfungiNezara viridulafood and beveragesbiology.organism_classificationVicia fabaVicia fabaSettore AGR/11 - Entomologia Generale E Applicata030104 developmental biologychemistryAgronomyABANezara viridulaShootABA; hydrogen peroxide; Nezara viridula; photosynthesis; salicylic acid; Vicia faba; water deficitPlant hormone010606 plant biology & botany
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First outbreak of Pepper vein yellows virus infecting sweet pepper in Italy

2016

Sweet pepper (Capsicum annum) is an economically important crop worldwide, including Sicily where about 4,000 hectares are grown each year. In October 2015, severe symptoms not previously reported by growers in the horticultural area of the province of Trapani (Sicily, Italy) were observed on sweet pepper plants in eight different greenhouses. Symptoms included upward leaf curling, internodal shortening and interveinal yellowing. Symptoms were more evident in the upper part of the plants. These symptoms were reminiscent of those caused by poleroviruses. In the greenhouse, symptoms were evident in about 35% of the plants. Three samples per greenhouse (24 in total) were collected for analysis.

0106 biological sciences0301 basic medicineSweet PeppersVeterinary medicinefood.ingredientHealth Toxicology and MutagenesisBegomoviruOutbreakSettore AGR/12 - Patologia VegetalePlant ScienceBiology01 natural sciencesVirusCropPolerovirus03 medical and health sciencesEmerging pathogen030104 developmental biologyfoodToLCNDVPlant virusBotanyPepperAgronomy and Crop Science010606 plant biology & botany
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Is the ballan wrasse (Labrus bergylta) two species? Genetic analysis reveals within-species divergence associated with plain and spotted morphotype f…

2016

Abstract The ballan wrasse (Labrus bergylta) is a marine fish belonging to the family Labridae characterized by 2 main morphotypes that occur in sympatry: spotty and plain. Previous studies have revealed differences in their life‐history traits, such as growth and maturation; however, the genetic relationship between forms is presently unknown. Using 20 recently developed microsatellite markers, we conducted a genetic analysis of 41 and 48 spotty and plain ballan wrasse collected in Galicia (northwest Spain). The 2 morphotypes displayed highly significant genetic differences to each other (F ST = 0.018, P < 0.0001). A similar degree of genetic differentiation (F ST = 0.025, P < 0.0001) was …

0106 biological sciences0301 basic medicineSympatryballan wrasseShort CommunicationZoologyGenetic relationshipLabrus bergyltaDNA Mitochondrial010603 evolutionary biology01 natural sciencesGenetic analysismicrosatellitesPerciformes03 medical and health sciencesAnimalsbiologyGenetic heterogeneitycolor morphotypesSequence Analysis DNALabrus bergyltabiology.organism_classificationPerciformesSympatry030104 developmental biologyspeciationSpainWrasseMicrosatelliteAnimal Science and ZoologyMicrosatellite RepeatsIntegrative Zoology
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Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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Marine Cryptophytes Are Great Sources of EPA and DHA

2017

Microalgae have the ability to synthetize many compounds, some of which have been recognized as a source of functional ingredients for nutraceuticals with positive health effects. One well-known example is the long-chain polyunsaturated fatty acids (PUFAs), which are essential for human nutrition. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are the two most important long-chain omega-3 (-3) PUFAs involved in human physiology, and both industries are almost exclusively based on microalgae. In addition, algae produce phytosterols that reduce serum cholesterol. Here we determined the growth rates, biomass yields, PUFA and sterol content, and daily gain of eight strains of marine…

0106 biological sciences0301 basic medicineTROPICAL AUSTRALIAN MICROALGAELINOLENIC ACIDrasvahapotPharmaceutical Sciencesterols01 natural scienceschemistry.chemical_compoundFunctional FoodDrug DiscoveryFood scienceBiomasslcsh:QH301-705.5Pharmacology Toxicology and Pharmaceutics (miscellaneous)functional foodsPOLYUNSATURATED FATTY-ACIDSchemistry.chemical_classificationnutraceuticalsFRESH-WATERPRODUCTIVITYbiologymicroalgae; polyunsaturated fatty acids; omega-3; omega-6; sterols; functional foods; nutraceuticalsCHOLESTEROLmicroalgaeNANNOCHLOROPSISPhytosterolsfood and beveragesEicosapentaenoic acidEicosapentaenoic AcidDocosahexaenoic acidFatty Acids Unsaturatedlipids (amino acids peptides and proteins)omega-3CryptophytaPolyunsaturated fatty acidpolyunsaturated fatty acidsDocosahexaenoic Acidsomega-6CHEMICAL-COMPOSITIONterveysvaikutteiset elintarvikkeetfatty acidsGas Chromatography-Mass SpectrometryArticleMARICULTURE03 medical and health sciencesNutraceuticalAlgaeFatty Acids Omega-6Fatty Acids Omega-31172 Environmental sciencessterolit010604 marine biology & hydrobiologyomega fatty acidsta1183ta1182GROWTH-RATEmikrolevätbiology.organism_classificationSterolomegarasvahapot030104 developmental biologyHuman nutrition416 Food Sciencelcsh:Biology (General)chemistry13. Climate actionDietary SupplementsStearidonic acidMarine Drugs; Volume 16; Issue 1; Pages: 3
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Trichoderma harzianum Strain T22 Modulates Direct Defense of Tomato Plants in Response to Nezara viridula Feeding Activity

2021

AbstractPlant growth-promoting fungi belonging to genus Trichoderma are known to help plants when dealing with biotic stressors by enhancing plant defenses. While beneficial effects of Trichoderma spp. against plant pathogens have long been documented, fewer studies have investigated their effect on insect pests. Here, we studied the impact of Trichoderma root colonization on the plant defense responses against stink bug feeding attack. For this purpose, a model system consisting of tomato plant, Solanum lycopersicum cv Dwarf San Marzano, Trichoderma harzianum strain T22 and the southern green stink bug, Nezara viridula, was used. We firstly determined stink bug performance in terms of rela…

0106 biological sciences0301 basic medicineTime FactorsTranscription GeneticGreen stink bugBeneficial soil microbes Jasmonic acid signaling pathway Pentatomidae Solanum lycopersicum Stink bugsCyclopentanesGenes PlantPlant Roots01 natural sciencesBiochemistryArticleHeteroptera03 medical and health scienceschemistry.chemical_compoundSolanum lycopersicumGene Expression Regulation PlantPentatomidaePlant defense against herbivoryAnimalsHerbivoryOxylipinsSymbiosisStink bugsEcology Evolution Behavior and SystematicsbiologyBeneficial soil microbesJasmonic acidfungifood and beveragesTrichoderma harzianumGeneral MedicinePentatomidaebiology.organism_classificationHorticulture030104 developmental biologychemistryNezara viridulaJasmonic acid signaling pathwayTrichodermaHypocrealesSeedsFemaleSolanumSignal Transduction010606 plant biology & botany
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Polyamine Oxidase 5 loss-of-function mutations in Arabidopsis thaliana trigger metabolic and transcriptional reprogramming and promote salt stress to…

2017

The family of polyamine oxidases (PAO) in Arabidopsis (AtPAO1-5) mediates polyamine (PA) back-conversion, which reverses the PA biosynthetic pathway from spermine, and its structural isomer thermospermine (tSpm), into spermidine and then putrescine. Here, we have studied the involvement of PA back-conversion in Arabidopsis salinity tolerance. AtPAO5 is the Arabidopsis PAO gene member most transcriptionally induced by salt stress. Two independent loss-of-function mutants (atpao5-2 and atpao5-3) were found to exhibit constitutively higher tSpm levels, with associated increased salt tolerance. Using global transcriptional and metabolomic analyses, the underlying mechanisms were studied. Stimul…

0106 biological sciences0301 basic medicineTranscription GeneticArabidopsis thalianaPhysiologyArabidopsisSperminePlant ScienceSodium Chloride01 natural scienceschemistry.chemical_compoundGene Expression Regulation PlantLoss of Function MutationArabidopsisPolyaminesMetabolitesArabidopsis thalianaPoliaminesAbscisic acidPrincipal Component AnalysisbiologyAgricultural SciencesSalt ToleranceMetabòlitsmetabolomicsPhenotypeBiochemistryMultigene FamilyMetabolomeCitric Acid CycleSalsCyclopentanes03 medical and health sciencesStress PhysiologicalOxylipinsRNA MessengerIonssalt toleranceArabidopsis ProteinsGene Expression ProfilingSodiumHydrogen PeroxideAgriculture Forestry and Fisheriesbiology.organism_classificationSpermidineGene Ontology030104 developmental biologychemistrythermosperminePutrescineSpermineSaltsOxidoreductases Acting on CH-NH2 Group DonorsTranscriptomejasmonatesPolyaminePolyamine oxidaseAbscisic Acid010606 plant biology & botany
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The Altered Expression of microRNA408 Influences the Arabidopsis Response to Iron Deficiency

2019

MicroRNAs contribute to the adaptation of plants to varying environmental conditions by affecting systemic mineral nutrient homeostasis. Copper and iron deficiencies antagonistically control the expression of Arabidopsis thaliana microRNA408 (miR408), which post-transcriptionally regulates laccase-like multicopper oxidase family members LAC3, LAC12, and LAC13. In this work, we used miR408 T-DNA insertion mutants (408-KO1 and 408-KO2) and a previously characterized transgenic line overexpressing miR408 (35S:408-14) to explore how miR408 influences copper- and iron-dependent metabolism. We observed that the altered expression of miR408 diminished plant performance and the activation of the ir…

0106 biological sciences0301 basic medicineTransgeneArabidopsisligninhydrogen peroxidePlant Sciencelcsh:Plant cultureMulticopper oxidase01 natural sciencesLignin03 medical and health sciencesiron deficiencyMicroRNA408ArabidopsisArabidopsis thalianalcsh:SB1-1110Iron deficiency (plant disorder)Original ResearchLaccasebiologyChemistryIron deficiencybiology.organism_classificationVascular bundleHydrogen peroxideCell biologymicroRNA408030104 developmental biologybiology.proteinCeruloplasmin010606 plant biology & botany
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Inhibition of NO Biosynthetic Activities during Rehydration of Ramalina farinacea Lichen Thalli Provokes Increases in Lipid Peroxidation

2019

Lichens are poikilohydrous symbiotic associations between a fungus, photosynthetic partners, and bacteria. They are tolerant to repeated desiccation/rehydration cycles and adapted to anhydrobiosis. Nitric oxide (NO) is a keystone for stress tolerance of lichens

0106 biological sciences0301 basic medicineTrebouxiaDiaphorase activityLichensHyphanitrate reductaseLipid peroxidationBOTANICAPlant ScienceNitrate reductase01 natural sciencesArticleRamalina farinaceaLipid peroxidation03 medical and health scienceschemistry.chemical_compoundstomatognathic systemnitric oxideMicroalgaeLichenskin and connective tissue diseaseslichensEcology Evolution Behavior and Systematics<i>Trebouxia</i>Ecologybiologyintegumentary systemnitric oxide synthaseNitric oxide synthasemicroalgaeBotanyNitric oxidelipid peroxidationbiology.organism_classificationThallusNitric oxide synthasestomatognathic diseases030104 developmental biologyBiochemistrychemistryQK1-989biology.proteinTrebouxiadiaphorase activityNitrate reductase010606 plant biology & botanyPlants
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