Search results for "explant"
showing 6 items of 86 documents
Olive embryo in vitro germination potential: Role of explant configuration and embryo structure among cultivars
2014
The in vitro germination of excised embryos can break dormancy rapidly and shorten the time required to produce seedlings, speeding up olive breeding programmes as well as rootstock production. In this study, the in vitro germination potential of four Sicilian olive cultivars was evaluated during two years of experiments, using explants with three different morphological configurations that represent three different degrees of embryo exposure: (1) intact stoneless seeds containing the embryo, the endosperm and the seed coat (Emb+En+SC), (2) seeds without the seed coat (Emb+En) and (3) naked, isolated embryos (seed coat and endosperm both removed: Emb). Differences were found in the germinat…
Micropropagation from inflorescence stems of the Spanish endemic plant Centaurea paui Loscos ex Willk. (Compositae)
1999
Tissue culture techniques have been established as a useful approach for ex situ conservation of rare, endemic or threatened plant species. This report describes the micropropagation of Centaurea paui Loscos ex Willk (Compositae), an extremely endangered plant species endemic to the Valencia Community (eastern Spain), as a conservation measure which does not cause damage to the wild plants used as explant source. Inflorescence nodal segments of C. paui were selected as explants for in vitro establishment. The best rate of shoot proliferation was obtained on Murashige and Skoog (MS) mineral medium supplemented with 0.5 mg/l 6-benzyladenine or with 2 mg/l kinetin. Maximum shoot elongation was…
Diflubenzuron-Induced alterations during in vitro development ofTenebrio molitor pupal integument
1987
The effects of diflubenzuron (DFB) in Tenebrio molitor pupae were first investigated on cuticle secretion induced by 20-hydroxyecdysone in vitro. The sternal integuments were treated by DFB either 3 days before culture or during culture. DFB, when applied before culture, did not prevent the molting hormone from inducing a new cuticle deposition by integument explants in vitro. However, this cuticle showed several architectural alterations and a thickness reduction. When applied during the culture in the presence of 20-hydroxyecdysone, DFB at high dose (≥ 20 μg/ml) was able to inhibit cuticle secretion, but lower doses (⩽ 10 μg/ml) resulted in epicuticle deposition. These observations confir…
Insulin synthesis in chick embryo retinas during development.
1994
Retinas of chick embryos contain insulin (1) and further, are capable of synthesizing it, as demonstrated by incubating retinas at different ages (7th–18th day) with [3H]leucine. The synthesized radioactive insulin was isolated and assayed by means of a HPLC procedure. The synthesis of insulin was found to be highest in the youngest retinas studied (day 7), afterwards it declined with age except for an increment found at 14–15 day. Explants of chick embryo retinas, cultured in vitro, rapidly degraded insulin. Nevertheless, the content of immunoreactive insulin in retinal explants diminished slowly with the age of culture, so that, after 8 days of incubation, it was about 60% of the content …
THE EFFECTS OF GROWTH REGULATORS ON IN VITRO AXILLARY SHOOT PROLIFERATION AND ROOTING OF ERICA MULTIFLORA
2012
Multiple shoots were induced on nodal segments of a mature plant of Erica multiflora L. Axillary shoots produced on uncontaminated explants were excised, segmented, and recultured on the same medium. The effect of Anderson medium, augmented with different concentrations of 2iP either singly or in combination with NAA, as potential medium for shoot multiplication by nodal segments was investigated. In the following experiment equal molar concentrations of four cytokinins (2iP, kinetin, zeatin, and BA) were studied for ability to induce axillary shoot development from single node stem segments. The highest rate of axillary shoot proliferation was induced on Anderson medium supplemented with 1…
Obtaining and research of callus mass of Gentiana lutea L roots
2015
The results of introduction to the culture in vitro Gentiana lutea L. are provided. The influence of phytohormones on growth processes of plant cells is researched, the optimal conditions for cultivation of Gentiana lutea L. are defined and chosen. Biomass extract is obtained and is researched on the presence in it the biologically active substances and antimicrobial activity.