Search results for "fagiterapia"

Bacteriophage Adherence to Mucus Mediates Preventive Protection against Pathogenic Bacteria

2019

The mucosal surfaces of animals are habitat for microbes, including viruses. Bacteriophages—viruses that infect bacteria—were shown to be able to bind to mucus. This may result in a symbiotic relationship in which phages find bacterial hosts to infect, protecting the mucus-producing animal from bacterial infections in the process. Here, we studied phage binding on mucus and the effect of mucin on phage-bacterium interactions. The significance of our research is in showing that phage adhesion to mucus results in preventive protection against bacterial infections, which will serve as basis for the development of prophylactic phage therapy approaches. Besides, we also reveal that exposure to m…

Microencapsulation of Salmonella-Specific Bacteriophage Felix O1 Using Spray-Drying in a pH-Responsive Formulation and Direct Compression Tableting o…

2019

The treatment of enteric bacterial infections using oral bacteriophage therapy can be challenging since the harsh acidic stomach environment renders phages inactive during transit through the gastrointestinal tract. Solid oral dosage forms allowing site-specific gastrointestinal delivery of high doses of phages, e.g., using a pH or enzymatic trigger, would be a game changer for the nascent industry trying to demonstrate the efficacy of phages, including engineered phages for gut microbiome modulation in expensive clinical trials. Spray-drying is a scalable, low-cost process for producing pharmaceutical agents in dry powder form. Encapsulation of a model Salmonella-specific phage (Myoviridae…

Fight evolution with evolution: plasmid‐dependent phages with a wide host range prevent the spread of antibiotic resistance

2013

The emergence of pathogenic bacteria resistant to multiple antibiotics is a serious worldwide public health concern. Whenever antibiotics are applied, the genes encoding for antibiotic resistance are selected for within bacterial populations. This has led to the prevalence of conjugative plasmids that carry resistance genes and can transfer themselves between diverse bacterial groups. In this study, we investigated whether it is feasible to attempt to prevent the spread of antibiotic resistances with a lytic bacteriophage, which can replicate in a wide range of gram‐negative bacteria harbouring conjugative drug resistance–conferring plasmids. The counter‐selection against the plasmid was sh…

Antibacterial Efficiency of Surface-Immobilized Flavobacterium-Infecting Bacteriophage

2019

Control of bacterial diseases by bacteriophages (phages) is gaining more interest due to increasing antibiotic resistance. This has led to technologies to attach phages on surfaces to form a biomaterial that can functionally display phages that interact with bacteria, to carry out successful infection cycles. Such a material could be applied in many environments, where the target pathogens are expected. Although this approach has been applied successfully in a few studies already, the basis of the antibacterial effect by the immobilized phages is unclear, and the interpretation of the results depends on the study. Here, we studied the phage attachment density, their detachment rate and infe…

Exploring phage-bacterium interactions : new ways to combat a fish pathogen

2014

Dataset for Mucin induces CRISPR-Cas defense in an opportunistic pathogen

2022

This dataset contains raw data points of (1) phage-bacterium co-evolution experiment (flavobacterium columnare colony counts, phage counts, bacterial growth characteristics and number of CRISPR-Cas spacers) and (2) from competition experiment between Flavobacterium columnare and Aeromonas sp. For detailed method description, see the attached Documentation file.

Expert Opinion on Three Phage Therapy Related Topics: Bacterial Phage Resistance, Phage Training and Prophages in Bacterial Production Strain

2018

Expert Opinion on Three Phage Therapy Related Topics: Bacterial Phage Resistance, Phage Training and Prophages in Bacterial Production Strain

2018

Phage therapy is increasingly put forward as a “new” potential tool in the fight against antibiotic resistant infections. During the “Centennial Celebration of Bacteriophage Research” conference in Tbilisi, Georgia on 26–29 June 2017, an international group of phage researchers committed to elaborate an expert opinion on three contentious phage therapy related issues that are hampering clinical progress in the field of phage therapy. This paper explores and discusses bacterial phage resistance, phage training and the presence of prophages in bacterial production strains while reviewing relevant research findings and experiences. Our purpose is to inform phage therapy stakeholders such as po…

Probing the interplay of microbes and medicine in a hospital setting using an individual-based model

2014

Tämän tutkielman tavoitteena oli kehittää graafisen käyttöliittymän omaava monipuolinen simulaatioympäristö, jolla voidaan mallintaa bakteerien leviämistä sairaalaympäristössä. Ohjelma mahdollistaa noin neljänkymmenen sairaalaparametrin asettamisen ja tulosten purkamisen taulukkomuotoon. Kyseisessä yksilöpohjaisessa mallissa keskitytään sairaalatyöntekijöiden ja potilaiden välisiin vuorovaikutuksiin. Työntekijät toimivat vektoreina, levittäen bakteeri-infektioita potilaiden välillä. Bakteerimallinnus yksittäisen potilaan sisällä on toteutettu matemaattisesti. Bakteerit voivat kilpailla keskenään ja muuntua antibiooteille vastustuskykyisiksi; tällöin voidaan hyödyntää faagiterapiaa, jossa in…

Analysis of the therapeutical properties of a phage against Staphylococcus aureus pig isolates, and phage isolation against Pseudomonas aeruginosa fr…

2017

Antibioottiresistenttiys on kasvava ongelma, jonka selättämiseksi tarvitaan uusia keinoja. Prekliinisten tutkimustulosten perusteella bakteereja infektoivien virusten eli faagien on havaittu olevan hyvä vaihtoehto antibiooteille. Sairaalanäytteestä löydettyä metisilliinille resistenttiä Staphylococcus aureus (MRSA) -kantaa vastaan eristetty bakteriofagi karakterisoitiin sekvensoimalla ja läpäisyelektronimikroskoopilla, jotta sen mahdollista terapeuttista käyttöä voitaisiin selvittää. Faagilla havaittiin olevan keskikokoinen 43,098 bp:n pituinen genomi ja faagin morfologian perusteella se lukeutuu Siphoviridae-heimoon kuuluviin hännällisiin faageihin. Faagi puhdistettiin anioninvaihtokromato…