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showing 10 items of 16583 documents

Main effects of human saliva on flavour perception and the potential contribution to food consumption.

2018

Part of: Nutrition Society Winter Meeting 2017; Whole saliva is a mixture composed by the secretions of the major and minor salivary glands and the crevicular fluid, bacteria, cells and food debris. Its properties (flow and composition) are highly intra- and inter-individually dependent and reflect the health status of individuals. Saliva plays a key role in the eating process and on the perception of flavour. Flavour corresponds to the combined effect of taste sensations, aromatics and chemical feeling factors evoked by food in the oral cavity. It is a key determinant of food consumption and intake. This review summarises the evidence about the role of saliva in flavour perception and its …

0301 basic medicineTasteSalivaFood intakemedia_common.quotation_subjectFlavourFood consumptionMedicine (miscellaneous)BiologyCrevicular fluid03 medical and health sciencesEating0404 agricultural biotechnologyhuman salivaPerceptionmedicineHumansFood scienceSalivamedia_common030109 nutrition & dieteticsNutrition and Dieteticsflavour perceptionTaste Perception04 agricultural and veterinary sciencesFeeding Behaviormedicine.disease040401 food scienceObesitynutritional statusfood consumptionTaste[SDV.AEN]Life Sciences [q-bio]/Food and NutritionThe Proceedings of the Nutrition Society
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Hsp60 quantification in human gastric mucosa shows differences between pathologies with various degrees of proliferation and malignancy grade

2021

Background: Stomach diseases are an important sector of gastroenterology, including proliferative benign

0301 basic medicineTechnologyPathologymedicine.medical_specialtyQH301-705.5QC1-999intestinal metaplasia03 medical and health sciences0302 clinical medicinechaperone systemMetaplasiamedicineGastric mucosaGeneral Materials ScienceBiology (General)QD1-999Instrumentationhealth care economics and organizationsFluid Flow and Transfer Processesbusiness.industryChaperone system Gastric carcinogenesis Gastric dysplasia Gastritis Hsp60 Intestinal metaplasiaSettore BIO/16 - Anatomia UmanaTPhysicsProcess Chemistry and TechnologyStomachdigestive oral and skin physiologygastritisGeneral EngineeringIntestinal metaplasiagastric dysplasiaEngineering (General). Civil engineering (General)Hsp60medicine.diseasedigestive system diseasesComputer Science ApplicationsChemistryGastric Dysplasia030104 developmental biologymedicine.anatomical_structureHyperplastic PolypDysplasia030220 oncology & carcinogenesisgastric carcinogenesisTA1-2040medicine.symptomGastritisbusiness
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Editorial for the Special Issue “Extracellular Chaperones and Related miRNA as Diagnostic Tools of Chronic Diseases”

2021

Molecular chaperones are a family of proteins that are highly conserved during phylogenesis [...]

0301 basic medicineTechnologyQH301-705.5mRNAQC1-999Computational biologyBiologyDiagnostic tools03 medical and health sciences0302 clinical medicinemicroRNAExtracellularchaperoneGeneral Materials ScienceBiology (General)QD1-999InstrumentationFluid Flow and Transfer ProcessesSettore BIO/16 - Anatomia UmanaTPhysicsProcess Chemistry and TechnologyGeneral EngineeringEngineering (General). Civil engineering (General)Computer Science ApplicationsChemistryn/a030104 developmental biologyPhylogenesisTA1-2040030217 neurology & neurosurgeryApplied Sciences
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Identification and Quantification of Valuable Compounds in Red Grape Seeds

2021

Grape seeds are a by-product of the wine industry. They represent 38–52% of grape pomace and about 5% of the weight of grapes. The main objective of this study is to establish some important characteristics of grape seeds from red varieties cultivated in Romania. The analyzed grape varieties were Cabernet Sauvignon, Merlot, Pinot noir, Burgund Mare, Cadarcă, Syrah, Novac. The grape seeds were dried and ground and the following determinations were made: determination of total polyphenol content, antioxidant capacity, antiradical capacity and determination of phenolic compounds. The analyses were performed on the first day after obtaining the grape extract, on the 14th day and the 30th day. T…

0301 basic medicineTechnologyQH301-705.5natural productsQC1-999antioxidant capacityBiology03 medical and health sciencesGrape extractantiradical capacityGeneral Materials ScienceBiology (General)QD1-999InstrumentationpolyphenolsFluid Flow and Transfer Processes030109 nutrition & dieteticsTPhysicsProcess Chemistry and TechnologyGeneral EngineeringPomaceEngineering (General). Civil engineering (General)Computer Science ApplicationsChemistryHorticultureAntioxidant capacity030104 developmental biologyPolyphenolTA1-2040biotechnologyWine industryApplied Sciences
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Rett Syndrome Mutant Neural Cells Lacks MeCP2 Immunoreactive Bands.

2016

Dysfunctions of MeCP2 protein lead to various neurological disorders such as Rett syndrome and Autism. The exact functions of MeCP2 protein is still far from clear. At a molecular level, there exist contradictory data. MeCP2 protein is considered a single immunoreactive band around 75 kDa by western-blot analysis but several reports have revealed the existence of multiple MeCP2 immunoreactive bands above and below the level where MeCP2 is expected. MeCP2 immunoreactive bands have been interpreted in different ways. Some researchers suggest that multiple MeCP2 immunoreactive bands are unidentified proteins that cross-react with the MeCP2 antibody or degradation product of MeCP2, while others…

0301 basic medicineThreonineHeredityMethyl-CpG-Binding Protein 2Genetic LinkageMutantFluorescent Antibody TechniqueSocial Scienceslcsh:MedicinePC12 CellsBiochemistryEpitopeImmunoenzyme TechniquesCell FusionNeuroblastomaFluorescence MicroscopyAnimal CellsMedicine and Health SciencesPsychologyPost-Translational ModificationPhosphorylationAmino Acidslcsh:ScienceCells CulturedCross ReactivityNeuronsStainingMicroscopyMultidisciplinaryReverse Transcriptase Polymerase Chain ReactionOrganic CompoundsCell StainingLight MicroscopyTransfectionChemistryX-Linked TraitsSex LinkagePhysical SciencesCellular TypesResearch ArticleCell signalingCell Physiologycongenital hereditary and neonatal diseases and abnormalitiesBlotting WesternImmunologyRett syndromeBiologyReal-Time Polymerase Chain ReactionResearch and Analysis MethodsMECP203 medical and health sciencesNeurologiaAntigenHydroxyl Amino Acidsmental disordersmedicineRett SyndromeGeneticsAnimalsHumansRNA MessengerClinical GeneticsHEK 293 cellsOrganic Chemistrylcsh:RChemical CompoundsBiology and Life SciencesProteinsCell Biologymedicine.diseaseMolecular biologyRatsnervous system diseases030104 developmental biologyHEK293 CellsSpecimen Preparation and TreatmentCellular NeuroscienceMutationDevelopmental PsychologyMalaltieslcsh:QNeuroscience
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Hypoxia-Induced miR-675-5p Supports β-Catenin Nuclear Localization by Regulating GSK3-β  Activity in Colorectal Cancer Cell Lines

2020

The reduction of oxygen partial pressure in growing tumors triggers numerous survival strategies driven by the transcription factor complex HIF1 (Hypoxia Inducible Factor-1). Recent evidence revealed that HIF1 promotes rapid and effective phenotypic changes through the induction of non-coding RNAs, whose contribution has not yet been fully described. Here we investigated the role of the hypoxia-induced, long non-coding RNA H19 (lncH19) and its intragenic miRNA (miR-675-5p) into HIF1-Wnt crosstalk. During hypoxic stimulation, colorectal cancer cell lines up-regulated the levels of both the lncH19 and its intragenic miR-675-5p. Loss of expression experiments revealed that miR-675-5p inhibitio…

0301 basic medicineTranscription factor complexKaplan-Meier Estimatelcsh:Chemistry0302 clinical medicineGSK-3poxiahylcsh:QH301-705.5long non-coding H19Spectroscopybeta CateninKinaseChemistryGeneral MedicineCell HypoxiaComputer Science ApplicationsCell biologyGene Expression Regulation Neoplastic030220 oncology & carcinogenesisColorectal NeoplasmsProtein BindingActive Transport Cell Nucleuscolorectal cancermiR-675TransfectionCatalysisArticleInorganic Chemistry03 medical and health sciencesCell Line TumormicroRNAGene silencingHumansPhysical and Theoretical ChemistryMolecular BiologyGlycogen Synthase Kinase 3 betahypoxiaOrganic ChemistryRNAComputational Biologyβ-cateninHCT116 CellsMicroRNAs030104 developmental biologylcsh:Biology (General)lcsh:QD1-999Microscopy FluorescenceCateninMutationNuclear localization sequenceInternational Journal of Molecular Sciences
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Topoisomerase 1 inhibition suppresses inflammatory genes and protects from death by inflammation

2015

Unwinding DNA and unleasing inflammation Fighting infections often comes with collateral damage, which sometimes can be deadly. For instance, in septic shock, the overwhelming release of inflammatory mediators drives multi-organ failure. Rialdi et al. now report a potential new therapeutic target for controlling excessive inflammation: the DNA unwinding enzyme topoisomerase I (Top1) (see the Perspective by Pope and Medzhitov). Upon infection, Top1 specifically localizes to the promoters of pathogen-induced genes and promotes their transcription by helping to recruit RNA polymerase II. Pharmacological inhibition of Top1 in a therapeutic setting increased survival in several mouse models of s…

0301 basic medicineTranscription GeneticType IInbred C57BLmedicine.disease_causeSendai virusMicePiperidinesTranscription (biology)Influenza A virusInnate2.1 Biological and endogenous factorsPositive Transcriptional Elongation Factor BAetiologyMultidisciplinaryAzepinesStaphylococcal InfectionsEbolavirusInfectious DiseasesDNA Topoisomerases Type IInfluenza A virusEbolaHost-Pathogen InteractionsPneumonia & InfluenzaRNA Polymerase IImedicine.symptomInfectionTranscriptionStaphylococcus aureusGeneral Science & TechnologyInflammationBiologyVaccine Related03 medical and health sciencesImmune systemGeneticImmunityBiodefenseGeneticsmedicineAnimalsHumansGeneFlavonoidsInflammationInnate immune systemPreventionHEK 293 cellsImmunityInterferon-betaHemorrhagic Fever EbolaTriazolesImmunity InnateMice Inbred C57BLEmerging Infectious DiseasesGood Health and Well BeingHEK293 Cells030104 developmental biologyGene Expression RegulationImmunologyCancer researchHemorrhagic FeverCamptothecinTopoisomerase I InhibitorsTopotecanDNA TopoisomerasesScience
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Tubercle disease of sugar beet roots (Beta vulgaris) found in Poland is neither caused by Xanthomonas beticola nor by tumorigenic Agrobacterium/Rhizo…

2018

Symptoms of tubercle disease known also as Xanthomonas gall were noticed in Poland in 2014–2017. Roots with disease symptoms showed the reduction of sucrose content compared to the healthy ones. In the literature, there are two gall diseases described: tubercle disease (also known as Xanthomonas gall) and crown gall, which is caused by Rhizobium radiobacter (syn. Agrobacterium tumefaciens). None of the bacterial strains isolated from the malformed tissues were identified as the bacterial pathogen responsible for causing Xanthomonas gall or crown gall. The result was confirmed by searching for the presence of T-DNA, which was not found. Our conclusion is that the tubercle disease observed in…

0301 basic medicineTubercleAgrobacteriumPlant ScienceHorticultureRhizobium radiobacterdigestive systemMicrobiology03 medical and health sciencesfluids and secretionsTubercle diseaseXanthomonasGallPathogenbiologySugar beetdigestive oral and skin physiologyXanthomonas gallAgrobacterium tumefaciensbiology.organism_classificationdigestive system diseases030104 developmental biologyAgrobacterium tumefaciensRhizobiumSugar beetAgronomy and Crop ScienceJournal of Plant Diseases and Protection
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Iron-loaded transferrin (Tf) is detrimental whereas iron-free Tf confers protection against brain ischemia by modifying blood Tf saturation and subse…

2018

Despite transferrin being the main circulating carrier of iron in body fluids, and iron overload conditions being known to worsen stroke outcome through reactive oxygen species (ROS)-induced damage, the contribution of blood transferrin saturation (TSAT) to stroke brain damage is unknown. The objective of this study was to obtain evidence on whether TSAT determines the impact of experimental ischemic stroke on brain damage and whether iron-free transferrin (apotransferrin, ATf)-induced reduction of TSAT is neuroprotective. We found that experimental ischemic stroke promoted an early extravasation of circulating iron-loaded transferrin (holotransferrin, HTf) to the ischemic brain parenchyma.…

0301 basic medicineU-PAGE urea-polyacrylamide gel electrophoresisMaleClinical BiochemistryExperimental strokeBiochemistryBrain IschemiaBrain ischemia0302 clinical medicineADC apparent diffusion coefficientApotransferrinDWI diffusion-weighted imagingTANDEM-1 Thrombolysis and Deferoxamine in Middle Cerebral Artery Occlusion clinical trialrHTf rat HTfrATf rat ATflcsh:QH301-705.5chemistry.chemical_classificationNeuronslcsh:R5-920ChemistryTransferrinExtravasationNS21 a medium supplement to grow neuronspDAPK-1 phosphorylated anti-death-associated protein kinase 1NeuroprotectionStrokeWB Western blotFemalemedicine.symptomlcsh:Medicine (General)Research PaperhHTf human HTfPC12 cell line derived from a pheochromocytoma of the rat adrenal medullamedicine.medical_specialtyIron OverloadBBB blood-brain barrierNMDAR N-methyl-D-aspartate receptorDCF dihydrofluoresceinIronWGA wheat germ agglutininHTf holotransferrinTransferrin receptorBrain damageTfR transferrin receptorDeferoxamineNeuroprotectionPI propidium iodide03 medical and health sciencesBrain damageCM conditioned mediumROS reactive oxygen speciesInternal medicine4-HNE 4-hydroxynonenalTf transferrinReceptors TransferrinmedicineFeRhoNoxTM-1 probe to detect Fe2+AnimalsHumansATf apotransferrinCM-H2DCFDA 5-chloromethyl-27-dichlorodihydrofluorescein diacetateMCAO middle cerebral artery occlusionDMT-1 divalent metal transporterB-27 a medium supplement to grow neuronsReactive oxygen speciesNMDA N-methyl-D-aspartateTSAT blood transferrin saturationTransferrin saturationBlood transferrin saturation (TSAT)Organic ChemistryNIR near infraredReactive oxygen species (ROS)medicine.diseasepMCAO permanent middle cerebral artery occlusionRatsPWI perfusion-weighted imaging030104 developmental biologyEndocrinologylcsh:Biology (General)TransferrinDAPK-1 anti-death-associated protein kinaseOGD oxygen/glucose deprivationTTC 235-triphenyl-tetrazolium chlorideLipid PeroxidationMCA middle cerebral arteryApoproteinsReactive Oxygen SpeciesMRI magnetic resonance imagingtMCAO transient middle cerebral artery occlusion030217 neurology & neurosurgeryhATf human ATf
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Six Serum miRNAs Fail to Validate as Myotonic Dystrophy Type 1 Biomarkers.

2016

Myotonic dystrophy type 1 (DM1) is an autosomal dominant genetic disease caused by expansion of a CTG microsatellite in the 3' untranslated region of the DMPK gene. Despite characteristic muscular, cardiac, and neuropsychological symptoms, CTG trinucleotide repeats are unstable both in the somatic and germinal lines, making the age of onset, clinical presentation, and disease severity very variable. A molecular biomarker to stratify patients and to follow disease progression is, thus, an unmet medical need. Looking for a novel biomarker, and given that specific miRNAs have been found to be misregulated in DM1 heart and muscle tissues, we profiled the expression of 175 known serum miRNAs in …

0301 basic medicineUntranslated regionMalePathologyPhysiologylcsh:MedicineArtificial Gene Amplification and ExtensionDiseaseBioinformaticsBiochemistryPolymerase Chain Reaction0302 clinical medicineTrinucleotide RepeatsMedicine and Health SciencesMyotonic Dystrophylcsh:ScienceMusculoskeletal SystemMultidisciplinaryMusclesHematologyMiddle Aged3. Good healthBody FluidsNucleic acidsBlotting SouthernBloodGenetic DiseasesBiomarker (medicine)AnatomyResearch ArticleAdultmusculoskeletal diseasesmedicine.medical_specialtyBiologyResearch and Analysis MethodsMyotonic dystrophy03 medical and health sciencesExtraction techniquesmicroRNAmedicineGeneticsHumansNon-coding RNAMolecular Biology TechniquesGeneMolecular BiologyClinical GeneticsBiology and life sciencesGene Expression Profilinglcsh:Rmedicine.diseaseRNA extractionGene regulationGene expression profilingMicroRNAs030104 developmental biologySkeletal MusclesRNAlcsh:QGene expressionAge of onset030217 neurology & neurosurgeryBiomarkersPLoS ONE
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