Search results for "force"
showing 10 items of 3423 documents
A mucosal pellicle modifies the physical properties of epithelial
2017
International audience
International Scanning Probe Microscopy Conference
2016
International audience
Technological innovation around protein and cell biochip for diagnosis: a translational research from nanoworld to patient
2009
International audience; A great challenge in biosensors and diagnosis devices relies on the way to reconstitute relevant biological mechanisms on surface of the biochips and which analytical tools are convenient to provide accurate and rapid information on the structures and function of molecules attached to this surface. A better control in the realization of biochips can be obtained in combining different complementary approaches while always keeping in mind the biological key point. Researches in CLIPP are focused towards this objective. Conception, realization and characterization of protein and cell chips are presented. We detail different strategies of materials engineering1,2,3, chem…
Cell imaging connected with protein mapping to investigate glyphosate induced toxicity on human keratinocytes
2009
During the skin ageing which is accelerated by environmental factors, the generated reactive oxygen species make unstable the "proliferation/differentiation/apoptosis" coupling of epidermal cells. A biochemical study, previously realized on human keratinocyte cultures treated by a pesticide, glyphosate, allowed the validation of an in vitro model of skin ageing. In this work, we propose to study the loss of HaCaT cell integrity appearing after this chemical induced-oxidative stress. An original approach, combining a micro- to nanoscale cell characterization and analysis of fluctuating protein expression, is proposed: a) the exploration of cell growth and morphology through fluorescence and …
Adaptations neuromusculaires des muscles extenseurs du genou : contractions fatigantes uni- vs bi-latérales
2010
The aim of this work was to determine i) the influence of the level of absolute force on the duration of the endurance time, and subsequent neuromuscular alterations in same individual at the end of an exercise performed at the same relative intensity, with one or two legs, ii) the influence of central drive on the force capacity production of the knee extensor muscles during unilateral (UL) vs. bilateral (BL) contractions, iii) activation mechanisms evolution for a serie of submaximal evoked contractions by electrostimulation (EMS). The results of the first study confirm that the endurance limit depends on the level of absolute force for the same individual, contraction duration for UL is …
Ventilatory conditioning by self-stimulation in rats: A pilot study
1994
International audience; This article describes an experimental attempt to condition breathing pattern in rats. In this experiment, a freely moving rat was first rewarded by an electrical stimulation of the medial forebrain bundle whenever inspiratory duration (TI) exceeded 300 ms. A bidirectional control was then used: TIs longer than 400 ms were rewarded, and then TIs shorter than 300 ms were rewarded. The frequency of TIs longer than 300 ms increased when this event was rewarded, further increased when TIs above 400 ms were rewarded, and decreased during reversal conditioning (TI < 300 ms). At the beginning of the experiment, stimulation caused increased arousal and motor activity, but af…
Etude dynamique et structurale de biomolécules par microscopie à force atomique HS-AFM : application à une petite protéine de choc thermique sHsp
2012
The atomic force microscopy (AFM) gives access to the topography of organic and inorganic samplesat the atomic scale. The latest innovations offer the possiblity to understand the sample nano-mechanicalproperties (elasticity, adhesion...). Its feature set allows overcoming the demands of nanotechnology,both in the fields of physics, chemistry and biology.However, understanding biological processes require faster acquisitions for the atomic forcemicroscopy, less than a second per frame. As conventional equipment does not offer the possibility toovercome the constraint of time for dynamical studies, a prototype of high-speed atomic forcemicroscope (HS-AFM) was developed in partnership with Pr…
Le juge et l'efficacité du contrat en droit comparé interne
2020
International audience; La force obligatoire du contrat est proclamé comme un principe cardinal du droit des contrats. Elle s'applique tant aux contrats administratifs qu'aux contrats de droit privé car elle est nécessaire à la sécurité juridique que recherchent les contractants. De prime abord, les parties doivent respecter les termes de leur contrat et le juge doit y veiller. Mais l'office du juge du contrat l'autorise à intervenir de façon croissante dans le contrat. L'essentiel n'est- il pas que le contrat soit efficace, c'est-à-dire permette d'atteindre les objectifs visés par les parties contractantes, en dépit d'obstacles tels que des irrégularités vénielles, des aléas imprévisibles,…
Les forces de la vie (18e siècle)
1994
International audience
Une force lisible dans une forme
2012
International audience