Search results for "forensic"
showing 10 items of 1701 documents
Improved method to retain cytosolic reporter protein fluorescence while staining for nuclear proteins
2014
Staining of transcription factors (TFs) together with retention of fluorescent reporter proteins is hindered by loss of fluorescence using current available methods. In this study, it is shown that current TF staining protocols do not destroy fluorescent proteins (FPs) but rather that fixation is not sufficient to retain FPs in the cytosol of the permeabilized cells. In this article, a simple and reliable protocol is elaborated, which allows efficient TF and cytokine staining while retaining FPs inside fixed cells.
Electron microscopy of a double helical tubular filament in keyhole limpet (Megathura crenulata) hemolymph.
1992
A approximately 25 nm hollow double helical filament has been detected ultrastructurally in the cell-free supernatant from hemolymph of the keyhole limpet Megathura crenulata (Gastropoda: Prosobranchia: Fissurellidae). Subsequently, much higher concentrations of this material were found in the cell pellet from hemolymph. Both negative staining and thin sectioning have been performed in an attempt to obtain a preliminary structural characterization of this new filament. It is proposed that the filaments are released or secreted from blood hemocytes in response to bleeding, but it has not been possible to define absolutely an intracellular organelle containing this material. It is shown that …
Activation of TRK Genes in Ewingʼs Sarcoma Trk A Receptor Expression Linked to Neural Differentiation
1997
Trk receptors have been identified by immunohistochemical methods in primitive neuroectodermal tumor (PNET)/Ewing's sarcoma (ES). However, the presence of different members of the Trk family of receptors in PNET/ES has not been specified. We have examined whether Trk A, B, and C receptors are specifically expressed in ES both with and without features of neural differentiation. Ten ES tumors (five primary tumors of bone and five extraosseous tumors transplanted into nude mice) were investigated for expression of Trk receptors by immunohistochemistry and reverse transcription-polymerase chain reaction. One primary ES and the five grafted ES tumors exhibited signs of neural differentiation; t…
La autopsia en casos de mala praxis
2002
Después de una escueta referencia a la trascendencia de la práctica de las autopsias clínicas, se aborda el problema de la autopsia forense en los casos de mala praxis. Estos pueden ser de muy distinta naturaleza y de la misma forma, los indicios que dejen en el cadáver van a ser también muy variados. Un problema, que puede ser determinante para la investigación, es el momento en el que se practica la autopsia, que puede ser anterior o posterior a la interposición de la reclamación. Asimismo, el estado del cadáver va a condicionar los resultados, atendiendo fundamentalmente a la fase de putrefacción en que se encuentre. Al poner en relación los tipos de mala praxis con las variedades de mue…
Open science as a better gatekeeper for science and society: a perspective from neurolaw
2018
Results from cognitive neuroscience have been cited as evidence in courtrooms around the world, and their admissibility has been a challenge for the legal system. Unfortunately, the recent reproducibility crisis in cognitive neuroscience, showing that the published studies in cognitive neuroscience may not be as trustworthy as expected, has made the situation worse. Here we analysed how the irreproducible results in cognitive neuroscience literature could compromise the standards for admissibility of scientific evidence, and pointed out how the open science movement may help to alleviate these problems. We conclude that open science not only benefits the scientific community but also the le…
�ber die Verwendung von o-Carboxyphenylphosphat zum Nachweis saurer Phosphatase im Sperma
1969
Fur die Bestimmung der sauren Spermaphosphatase wird an Hand eines Vergleiches mit Phenylphosphat (Methode nachErdmann-Muller) als Substrat o-Carboxyphenylphosphat empfohlen. Die optimalen Reaktionsbedingungen werden festgestellt. Das Inkubationsmedium ist wie folgt zusammengesetzt: o-Carboxyphenylphosphat 2,5·10−3 M, Essigsaure/Natriumacetatpuffer pH 4,8 0,1 M, Probelosung (bei 2,5 ml-Ansatz). 0,1 ml.
The effectiveness of the RSID confirmatory test kit for human alpha amylase: the effects of environmental factors and substrate materials
2016
The investment in time and money that DNA analyses involves, provides a justification for forensic laboratories to carry out prior tests to ensure the presence of biological fluid in samples. The RSID kit has already been evaluated for forensic use, but its effectiveness on forensic casework samples is almost impossible to predict. Moreover, forensic reports presented before a court of law must justify the methods used to generate their findings. Consequently, trials to know how factors – such as the characteristics of the donor, the substrate and environmental conditions – can affect the kit’s efficacy are essential. This study evaluates some of these factors. For this aim, samples were ma…
High expression of progesterone receptor may be an adverse prognostic factor in oestrogen receptor-negative/progesterone receptor-positive breast can…
2021
Oestrogen receptor (ER)-negative (–) progesterone receptor (PgR)-positive (+) is the least common combination of steroid receptor expression observed in breast cancer. There are many controversies regarding the actual existence of ER–/PgR+ phenotype. In the current study, we aimed to perform comprehensive immunohistochemical re-evaluation of ER–/PgR+ breast cancers from multiple institutions. A total of 135 cases of ER–/PgR+ breast cancer were collected from 11 institutions from the period 2006–2020 and subsequently stained with three clinically validated anti-ER antibody clones: SP1 (Roche), 1D5 (Dako), and EP1 (Dako), and two anti-PgR antibody clones: 636 (Dako), and 1E2 (Roche). Clinicop…
Quando bisogna discostarsi dalle linee guida? I casi problematici nella prassi odontoiatrica
2018
Obiettivi Delineare vantaggi e limiti delle linee guida e dei riferimenti alle buone pratiche nella valutazione della condotta professionale dell’odontoiatra. Definire tipologie di pazienti e contesti operativi nei quali esse non risultano applicabili. Quest’obiettivo è coerente con i recenti sviluppi giurisprudenziali della Corte di Cassazione a Sezioni Unite del dicembre 2017 in tema di colpa e responsabilità dei sanitari. Materiali e metodi Si analizzano i riferimenti contenuti nella Legge 24/2017; quindi, in riferimento alla metodologia della responsabilità medico/odontoiatrica, si individuano i contesti della pratica clinica nei quali è più opportuno cercare di sviluppare buone pratich…
Detection of Duplicated Regions in Tampered Digital Images by Bit-Plane Analysis
2009
In this paper we present a new method for searching duplicated areas in a digital image. The goal is to detect if an image has been tampered by a copy-move process. Our method works within a convenient domain. The image to be analyzed is decomposed in its bit-plane representation. Then, for each bitplane, block of bits are encoded with an ASCII code, and a sequence of strings is analyzed rather than the original bit-plane. The sequence is lexicographically sorted and similar groups of bits are extracted as candidate areas, and passed to the following plane to be processed. Output of the last planes indicates if, and where, the image has been altered.