Search results for "forensic"
showing 10 items of 1701 documents
Ambiguity in legal language: its diagnosis and interpretation through forensic linguistics
2017
Como variante diastrática derivada del lenguaje general, el lenguaje jurídico experimenta en muchos casos problemas de interpretación similares a los de la lengua estándar: por su naturaleza específica, surgen no pocas veces dudas en cuanto a la percepción del significado preciso de un determinado texto (o fragmento de texto). Ante este problema se hace prioritaria la necesidad de recurrir al asesoramiento de peritos o expertos en diversas áreas de conocimiento relacionadas con dicho problema, uno de los cuales debería ser el perito lingüista, cuya actividad se desarrolla en el marco de la denominada lingüística forense. Planteada así la disyuntiva de interpretación, si la cuestión radica e…
SCD5-induced oleic acid production reduces melanoma malignancy by intracellular retention of SPARC and cathepsin B
2015
A proper balance between saturated and unsaturated fatty acids (FAs) is required for maintaining cell homeostasis. The increased demand of FAs to assemble the plasma membranes of continuously dividing cancer cells might unbalance this ratio and critically affect tumour outgrowth. We unveiled the role of the stearoyl-CoA desaturase SCD5 in converting saturated FAs into mono-unsaturated FAs during melanoma progression. SCD5 is down-regulated in advanced melanoma and its restored expression significantly reduced melanoma malignancy, both in vitro and in vivo, through a mechanism governing the secretion of extracellular matrix proteins, such as secreted protein acidic and rich in cysteine (SPAR…
Assessment of Escherichia coli B with enhanced permeability to fluorochromes for flow cytometric assays of bacterial cell function.
2002
Background Flow cytometry has become a choice methodology for microbiological research. However, functional cytometric assays in live bacteria are still limited. This is due, in part, to the cell wall impairing penetration of vital dyes in bacteria, thus imposing permeabilization procedures. These manipulations may affect cell physiology, provoke cell aggregation or lysis, and they are time-consuming. Escherichia coli B strains have been used for mutagenic assays because of an altered lipopolysaccharide that provokes increased membrane permeability. We assessed the use of these strains as possible alternatives for flow cytometric assays to avoid the permeabilization steps. Methods Suspensio…
Time-related changes in size of nuclei of pinealocytes in rats
1981
In a study of 96 adult male Sprague-Dawley rats, da- and time-related changes of the mean nuclear volume of pinealocytes were determined with the aim of testing the reproducibility of the karyometric findings described by Quay and Renzoni (1966). It is shown (i) that statistically significant differences exist between the central and peripheral mean volumes of pinealocyte nuclei/group of animals and time point (p less than 0.001), (ii) that the day and time related differences are statistically different in both regions (p less than 0.001), (iii) that in the center and periphery of the pineal body different diurnal patterns exist, and (iv) that the diurnal patterns are not parallel in the t…
PECAM-1 expression in human mesothelial cells: an in vitro study.
1996
Mesothelial cells are actively involved in inflammatory processes by expressing a set of cell adhesion molecules (CAMs). Transmigration of leukocytes into inflamed tissues requires a chemotactic stimulus and engagement of platelet-endothelial cell adhesion molecule-1 (PECAM-1). To investigate the kinetics involved in peritonitis, pure cultures of mesothelial cells are necessary. In previous studies, we have found that human mesothelial cells (HOMES) show a weak constitutive expression of PECAM-1, which cannot be further stimulated by cytokines. It is known that all serous cavities and body fluids contain numerous macrophages which strongly express this adhesion molecule. To identify the cel…
Effects of vinblastine, leucine, and histidine, and 3-methyladenine on autophagy in Ehrlich ascites cells.
1990
The microtubule inhibitor vinblastine causes accumulation of autophagic vacuoles in many cell types. In hepatocytes, many of the accumulated vacuoles are nascent, which has been interpreted to suggest that vinblastine acts by inhibiting the fusion of hydrolase-containing lysosomes with early autophagic vacuoles. However, our previous results suggested that, in Ehrlich ascites cells, vinblastine causes accumulation mainly of older autophagic vacuoles (AVs). This study was undertaken to further characterize the mode of action of vinblastine in these cells. The vinblastine-accumulated AVs were quantified by electron-microscopic morphometry. In addition, the effects of inhibitors of autophagic …
ANP (Atrial Natriuretic Peptide) presence in the heart of a tunicate, Ciona intestinalis.
2010
Atrial natriuretic peptide was found in the heart of vertebrates, we studied the ANP presence in the heart of Ciona intestinalis. This is animal is very important because of the its evolutionary position between invertebrates and vertebrates. ANP presence was only revealed in myoepithelial cells of the myocardium. Results suggest the hypothesis that ANP is present not only in the vertebrates but also in the invertebrates and in Ciona heart ANP might play a similar role like in the heart of vertebrates.
TGF-β superfamily signaling is essential for tooth and hair morphogenesis and differentiation
2007
Members of the transforming growth factor beta (TGF-beta) superfamily of signaling molecules are involved in the regulation of many developmental processes that involve the interaction between mesenchymal and epithelial tissues. Smad7 is a potent inhibitor of many members of the TGF-beta family, notably TGF-beta and activin. In this study, we show that embryonic overexpression of Smad7 in stratified epithelia using a keratin 5 promoter, results in severe morphogenetic defects in skin and teeth and leads to embryonic and perinatal lethality. To further analyze the functions of Smad7 in epithelial tissues of adult mice, we used an expression system that allowed a controlled overexpression of …
Komplikationen nach Zelltherapie
1989
Nach wie vor durfen tierische Frischzellpraparationen zur sog. Zellulartherapie verabreicht werden unter der Vorstellung einer allgemeinen Vitalisierung und Roborierung des Organismus, insbesondere des Immunsystems. Die Verwendung von Trockenzelltherapeutica hat das Bundesgesundheitsamt bereits 1987 vorlaufig verboten, nicht ohne darauf hinzuweisen, das auch gegen die sog. Frischzelltherapie schwerwiegende Bedenken bestunden, das ein generelles Verbot auch dieser Behandlungsweise jedoch nicht in seiner Kompetenz lage. Wiederholt waren z.T. lebensbedrohliche und auch todliche Komplikationen im Verlauf dieser Therapie beobachtet und beschrieben worden. Den bereits publizierten Beobachtungen w…
Adipose tissue, angiogenesis and angio-MIR under physiological and pathological conditions.
2019
Angiogenesis is a crucial process for the maintenance of normal tissue physiology and it is involved in tissue remodeling and regeneration. This process is essential for adipose tissue maintenance. The adipose tissue is composed by different cell types including stromal vascular cells as well as adipose stem cells (ASCs). In particular, ASCs are multipotent somatic stem cells that are able to differentiate and secrete several growth factors; they are recently emerging as a new cell reservoir for novel therapies and strategies in many diseases. Several studies suggest that ASCs have peculiar properties and participate in different disease-related processes such as angiogenesis. Furthermore, …