Search results for "functional"
showing 10 items of 4822 documents
Specific interaction of desthiobiotin lipids and water-soluble biotin compounds with streptavidin
1991
As shown for biotin lipids (Ref. 1), the formation of perfect 2-D crystalline streptavidin domains can also be observed in the plane of desthiobiotin lipid monolayers. The binding constant of streptavidin with desthiobiotin (Ka = 5·1013 mol−1) is lower than that with biotin (Ka = 1015 mol−1) (Ref. 2). By adding free biotin into the subphase a competitive replacement and a detaching of the streptavidin domains from the desthiobiotin lipid monolayer takes place. Streptavidin domains built at receptor lipid monolayers are still functional. As could be shown, there are two biotin binding sites at each protein molecule that are fully accessible to biotin (Ref. 1). This can be proven by the inter…
Mutation of a critical tryptophan to lysine in avidin or streptavidin may explain why sea urchin fibropellin adopts an avidin-like domain
1999
Sea urchin fibropellins are epidermal growth factor homologues that harbor a C-terminal domain, similar in sequence to hen egg-white avidin and bacterial streptavidin. The fibropellin sequence was used as a conceptual template for mutation of designated conserved tryptophan residues in the biotin-binding sites of the tetrameric proteins, avidin and streptavidin. Three different mutations of avidin, Trp-110-Lys, Trp-70-Arg and the double mutant, were expressed in a baculovirus-infected insect cell system. A mutant of streptavidin, Trp-120-Lys, was similarly expressed. The homologous tryptophan to lysine (W--K) mutations of avidin and streptavidin were both capable of binding biotin and bioti…
Locally convex quasi $C^*$-normed algebras
2012
Abstract If A 0 [ ‖ ⋅ ‖ 0 ] is a C ∗ -normed algebra and τ a locally convex topology on A 0 making its multiplication separately continuous, then A 0 ˜ [ τ ] (completion of A 0 [ τ ] ) is a locally convex quasi ∗-algebra over A 0 , but it is not necessarily a locally convex quasi ∗-algebra over the C ∗ -algebra A 0 ˜ [ ‖ ⋅ ‖ 0 ] (completion of A 0 [ ‖ ⋅ ‖ 0 ] ). In this article, stimulated by physical examples, we introduce the notion of a locally convex quasi C ∗ -normed algebra, aiming at the investigation of A 0 ˜ [ τ ] ; in particular, we study its structure, ∗-representation theory and functional calculus.
Maximal Operators with Respect to the Numerical Range
2018
Let $\mathfrak{n}$ be a nonempty, proper, convex subset of $\mathbb{C}$. The $\mathfrak{n}$-maximal operators are defined as the operators having numerical ranges in $\mathfrak{n}$ and are maximal with this property. Typical examples of these are the maximal symmetric (or accretive or dissipative) operators, the associated to some sesquilinear forms (for instance, to closed sectorial forms), and the generators of some strongly continuous semi-groups of bounded operators. In this paper the $\mathfrak{n}$-maximal operators are studied and some characterizations of these in terms of the resolvent set are given.
The Anthropologist as Deviant Modernizer: Felipe Landa Jocano’s Journey Through the Cold War, the Social Sciences, Decolonization, and Nation Buildin…
2021
This chapter traces the transnational journey of Philippine anthropologist Felipe Landa Jocano from the 1950s to the 1970s. Jocano was part of a group of Philippine social scientists who earned their post-graduate degrees at the University of Chicago after the Philippines became independent from the US. One of the pre-eminent scholars in the Philippines, Jocano’s professional and intellectual journey unfolded against the backdrop of the Cold War and decolonization. Jocano received funding from US institutions that contributed to the Cold War, and he was committed to structural functionalism and modernization theory. Yet he developed a decolonized approach to modernization that questioned th…
Analysis of Chromatin Structure and Composition
1989
Introduction Biochemistry, like many other sciences, is currently undergoing increasing specialization which is thought to be unavoidable because of the rapid progress within this field. Obviously education in Biochemistry and Molecular Biology is also affected. Consequently, the student may lose the ability to integrate his knowledge, which should be a requirement during the training of a scientist. The solution to this problem is quite easy in the case of theoretical courses because, here, the lecturer may include several 'integrative lessons' which give a global view of previously explained facts and place them within the general context of the course. However, in practical courses it is…
Synthesis of new cationic donor-stabilized phosphenium adducts and their unexpected P-substituent exchange reactions
2008
The reaction between two 1,3-dialkylimidazolium-2-carboxylates 1a and 1b and two different dichlorophosphines (RPCl(2), with R = Ph and NEt(2)) led to new donor-stabilized phosphenium adducts. When the reaction was performed with the 1,3-dimethylimidazolium-2-carboxylate 1a and PhPCl(2) in a 2:1 ratio, the phosphine 4a, bearing two imidazolium moieties, was obtained and led to 5a, after an anion exchange reaction with KPF(6), the latter being fully characterized by an X-ray structure analysis. In similar conditions, the bis-imidazolium phosphine or phosphene-di-ium, 4b, which is analogous to 4a, has been obtained by the addition of PhPCl(2) to the 1-dodecyl-3-methylimidazolium-2-carboxylate…
Comparing fMRI inter-subject correlations between groups using permutation tests
2018
AbstractInter-subject correlation (ISC) based analysis is a conceptually simple approach to analyze functional magnetic resonance imaging (fMRI) data acquired under naturalistic stimuli such as a movie. We describe and validate the statistical approaches for comparing ISCs between two groups of subjects implemented in the ISC toolbox, which is an open source software package for ISC-based analysis of fMRI data. The approaches are based on permutation tests. We validated the approaches using five different data sets from the ICBM functional reference battery tasks. First, we created five null datasets (one for each task) by dividing the subjects into two matched groups and assumed that no gr…
Update on mechanism and therapeutic implications of spinal cord stimulation and cerebral hemodynamics: A narrative review
2017
Spinal cord stimulation (SCS) is well known for its early role in the management of chronic pain, mainly failed back surgery syndrome (FBSS), spasticity, and bowel and bladder dysfunction. In more recent years, SCS has been proposed for patients suffering from refractory angina or peripheral vasculopathies in order to gain symptom relief, thus indicating some hemodynamic effect on the peripheral circulation. Taking into account this scientific observation, since the late1980s, researchers have started to investigate the potential effect of SCS on cerebral blood flow (CBF) regulation and its possible application in certain pathological settings dealing with vascular pattern dysfunction, such…
Subdifferential and conjugate calculus of integral functions with and without qualification conditions
2023
We characterize the subdifferential and the Fenchel conjugate of convex integral functions by means of respectively the approximate subdifferential and the conjugate of the associated convex normal integrands. The results are stated in Suslin locally convex spaces, and do not require continuity-type qualification conditions on the functions, nor special topological or algebraic structures on the index set. Consequently, when confined to separable Banach spaces, the characterizations of such a subdifferential are obtained using only the exact subdifferential of the given integrand but at nearby points. We also provide some simplifications of our formulas when additional continuity conditions…