Search results for "fusion"

RGD motifs on the surface of baculovirus enhance transduction of human lung carcinoma cells.

2006

Baculovirus vectors have been shown to enter a variety of mammalian cell lines and gene transfer with wild-type baculovirus (WT) has been demonstrated both in vitro and in vivo. Different protein motifs have been displayed on the viral surface to serve as ligands for cell-specific receptor molecules. We have generated recombinant baculovirus vectors displaying an RGD-motif, recognized by alphaV integrin, on the viral surface. The RGD motifs within the C-terminus of coxsackie virus A9 and human parechovirus 1 VP1 proteins were fused to the N-terminus of the major envelope glycoprotein, gp64, of Autographa californica multiple nucleopolyhedrovirus. The recombinant RGD-presenting viruses bound…

Ein Auswerteprogramm zur quantitativen Analyse von Messungen des alveolären Sauerstoffpartialdrucks (pAO2) mit der sauerstoffsensitiven 3He-MR-Tomogr…

2004

Purpose: To develop a software tool for quantitative analysis of alveolar oxygen partial pressure (p A O 2 ) as well as its time course during apnea. Material and Methods: T 1 -relaxation times of hyperpolarized 3 He are reduced by paramagnetic oxygen rendering 3 He-MRI sensitive to oxygen and thus allowing the assessment of the local oxygen partial pressure in the pulmonary airspaces. Oxygen-related relaxation and loss of polarization by RF-excitation can be discriminated by acquiring two image series with varying interscan delay and/or flip angles. Software was developed to calculate the p A O 2 and the decay rate in user-defined regions of interest (ROIs) automatically. Moreover, paramet…

Remote Ischemic Preconditioning Decreases Oxidative Lung Damage After Pulmonary Lobectomy

2017

BACKGROUND:During lobectomy in patients with lung cancer, the operated lung is often collapsed and hypoperfused. Ischemia/reperfusion injury may then occur when the lung is re-expanded. We hypothesized that remote ischemic preconditioning (RIPC) would decrease oxidative lung damage and improve gas e

Kontrastmittelverstärkte 3D-MR-Perfusion der Lunge: Einsatz paralleler Bildgebungstechniken bei gesunden Probanden

2004

PURPOSE Evaluation of lung perfusion by contrast-enhanced 3D MRI using partial parallel imaging techniques. MATERIALS AND METHODS Eight healthy volunteers were examined using a contrast-enhanced dynamic FLASH 3D sequence with partial parallel imaging technique at 1.5 T MRI with a TA of 1.5 sec. The whole lung was covered by 36 coronal slices. A ventral, middle and dorsal slice of each lung was manually segmented and signal-to-time curves were computed. For absolute quantification of blood flow through the right and left pulmonary artery, phase-contrast flow measurements were performed. RESULTS No significant difference was found between the signal intensity in the right (8.9 +/- 2.6) and le…

Determination Of Pulmonary Parameters (V̇A, $$ {D_{{L_{{O^2}}}}} $$ ) From Arterial O2 and CO2 Partial Pressures During Exercise

1984

The arterial O2 and CO2 partial pressures mainly depend on the ventilation-perfusion ratio VA/Q and on the O2 diffusing capacity-perfusion ratio DLO2/Q. According to RAHN’s VA/Q concept, the dependence of the alveolar O2 and CO2 partial pressures on the ventilation-perfusion ratio can be determined graphically, and displayed in the form of a diagram (2,3). However, the application of the RAHN diagram is limited by the fact that during the passage of the blood through the lung capillaries, a complete adjustment of the capillary O2 partial pressure to the alveolar value frequently does not occur. This is particularly valid for gas exchange during exercise. For this case, the alveolar-endcapil…

Elucidation of structure-function relationships in the lung: contributions from hyperpolarized 3 helium MRI

2002

Magnetic resonance imaging (MRI) using hyperpolarized 3helium (He) gas as the source of signal provides new physiological insights into the structure-function relationships of the lung. Traditionally, lung morphology has been visualized by chest radiography and computed tomography, whereas lung function was assessed by using nuclear medicine. As all these techniques rely on ionizing radiation, MRI has some inherent advantages. 3He MRI is based on 'optical pumping' of the 3He gas which increases the nuclear spin polarization by four to five orders of magnitude translating into a massive gain in signal. Hyperpolarized 3He gas is administered as an inhaled 'contrast agent' and allows for selec…

Helium-3-MRT der Lungenventilation: Erste klinische Anwendungen

1997

PURPOSE: of the study is the visualisation of normal pulmonary ventilation in healthy volunteers and the evaluation of abnormalities in patients with different lung diseases using 3He magnetic resonance imaging (3He-MRI). MATERIAL AND METHODS: Hyperpolarized 3He gas (V = 300 ml, p = 3 x 10(5) Pa, polarised to 35-45% by optical pumping, provided in special glass cells) was inhaled by eight healthy volunteers and ten patients with different lung diseases. A 3D FLASH sequence (TR = 11.8 ms; TE = 5 ms; matrix 144 x 256, FOV 350 mm, section thickness 7-10 mm, coronal orientation) was performed in a single breath-hold (22-42 s). Clinical and radiological examinations were available for correlatio…

2014

This paper is devoted to investigating stability in mean of partial variables for coupled stochastic reaction-diffusion systems on networks (CSRDSNs). By transforming the integral of the trajectory with respect to spatial variables as the solution of the stochastic ordinary differential equations (SODE) and using Itô formula, we establish some novel stability principles for uniform stability in mean, asymptotic stability in mean, uniformly asymptotic stability in mean, and exponential stability in mean of partial variables for CSRDSNs. These stability principles have a close relation with the topology property of the network. We also provide a systematic method for constructing global Lyapu…

Stability analysis for stochastic hybrid systems: A survey

2014

This survey addresses stability analysis for stochastic hybrid systems (SHS), which are dynamical systems that combine continuous change and instantaneous change and that also include random effects. We re-emphasize the common features found in most of the models that have appeared in the literature, which include stochastic switched systems, Markov jump systems, impulsive stochastic systems, switching diffusions, stochastic impulsive systems driven by renewal processes, diffusions driven by Lévy processes, piecewise-deterministic Markov processes, general stochastic hybrid systems, and stochastic hybrid inclusions. Then we review many of the stability concepts that have been studied, inclu…

Global stability of coupled Markovian switching reaction–diffusion systems on networks

2014

Abstract In this paper, we investigate the stability problem for some Markovian switching reaction–diffusion coupled systems on networks (MSRDCSNs). By using the Lyapunov function, we establish some novel stability principles for stochastic stability, asymptotically stochastic stability, globally asymptotically stochastic stability and almost surely exponential stability of the MSRDCSNs. These stability principles have a close relation to the topology property of the network. We also provide a systematic method for constructing global Lyapunov function for these MSRDCSNs by using graph theory. The new method can help analyze the dynamics of complex networks.