Search results for "genesis"
showing 10 items of 12136 documents
Phytotoxic Metabolites Isolated from Neufusicoccum batangarum, the Causal Agent of the Scabby Canker of Cactus Pear (Opuntia ficus-indica L.)
2020
Six phytotoxins were obtained from the culture filtrates of the ascomycete Neofusicoccum batangarum, the causal agent of the scabby canker of cactus pear (Opuntia ficus-indica L.) in minor Sicily islands. The phytotoxins were identified as (&minus
Multiple-stressor effects of warming and acidification on the embryonic development of an estuarine fiddler crab
2021
Made available in DSpace on 2021-06-25T10:56:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-06-05 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Predicted effects of anthropogenic climate change on estuarine and coastal organisms are complex, and early life history stages of calcified ectotherms are amongst the most sensitive groups. Despite the importance of understanding their vulnerability, we lack information on the effects of multiple stressors on the embryonic development of estuarine and burrowing organisms, mainly mangrove-associated species. Here, we determined the combined effects of elevated temperature and decreased pH on the embryonic development of th…
Boric acid toxicity to the German cockroach, Blattella germanica: Alterations in midgut structure, and acetylcholinesterase and glutathione S-transfe…
2006
0048-3575 doi: DOI: 10.1016/j.pestbp.2005.05.002; Oral toxicity of boric acid, an inorganic insecticide, was evaluated on German cockroach, Blattella germanica L. (Dictyoptera, Blattellidae). Newly emerged adults were exposed to various concentrations of boric acid incorporated into the diet. Results showed that treated insects exhibited toxic symptoms with a dose-dependent mortality. Histological study of midgut revealed alterations in the epithelial cells and a signiWcant increase in the epithelium thickness. In a second series of experiments, the compound was investigated on the activities of acetylcholinesterase (AChE) and glutathione S-transferases (GSTs). Data showed that the compound…
Effect of methylparaben in Artemia franciscana.
2017
In this study, the toxicity of methylparaben (MeP) an emerging contaminant, was analysed in the sexual species Artemia franciscana, due to its presence in coastal areas and marine saltworks in the Mediterranean region. The acute toxicity (24 h-LC50) of MeP in nauplii was tested and its chronic effect (9 days) evaluated by measuring survival and growth under two sublethal concentrations (0.0085 and 0.017 mg/L). Also, the effect on several key enzymes involved in: antioxidant defences (catalase (CAT) and gluthathion-S-transferase (GST)), neural activity (cholinesterase (ChE)) and xenobiotic biotransformation (carboxylesterase (CbE), was assessed after 48 h under sublethal exposure. The result…
To Swim or Not to Swim: Potential Transmission of Balaenophilus manatorum (Copepoda: Harpacticoida) in Marine Turtles
2017
Species of Balaenophilus are the only harpacticoid copepods that exhibit a widespread, obligate association with vertebrates, i.e., B. unisetus with whales and B. manatorum with marine turtles and manatees. In the western Mediterranean, juveniles of the loggerhead sea turtle, Caretta caretta are the only available hosts for B. manatorum, which has been found occurring at high prevalence (>80%) on them. A key question is how these epibionts are transmitted from host to host. We investigated this issue based on experiments with live specimens of B. manatorum that were cultured with turtle skin. Specimens were obtained from head-started hatchlings of C. caretta from the western Mediterranean. …
Carbon, iron and sulphur cycling in the sediments of a Mediterranean lagoon (Ghar El Melh, Tunisia)
2019
Coastal lagoon sediments are important for the biogeochemical carbon cycle at the land-ocean transition, as they form hotspots for organic carbon burial, as well as potential sites for authigenic carbonate formation. Here, we employ an early diagenetic model to quantify the coupled redox cycling of carbon, iron and sulphur in the sediments of the shallow Ghar El Melh (GEM) lagoon (Tunisia). The model simulated depth profiles show a good correspondence with available pore water data (dissolved inorganic carbon, NH 4 + , total alkalinity, Ca 2+ , Fe 2+ and SO 4 2− ) and solid phase data (organic matter, pyrite, calcium carbonate and iron (oxyhydr)oxides). This indicates that the model is abl…
Selection for Robustness in Mutagenized RNA Viruses
2007
Mutational robustness is defined as the constancy of a phenotype in the face of deleterious mutations. Whether robustness can be directly favored by natural selection remains controversial. Theory and in silico experiments predict that, at high mutation rates, slow-replicating genotypes can potentially outcompete faster counterparts if they benefit from a higher robustness. Here, we experimentally validate this hypothesis, dubbed the ‘‘survival of the flattest,’’ using two populations of the vesicular stomatitis RNA virus. Characterization of fitness distributions and genetic variability indicated that one population showed a higher replication rate, whereas the other was more robust to mut…
Sex versus parthenogenesis: A transcriptomic approach of photoperiod response in the model aphid Acyrthosiphon pisum (Hemiptera: Aphididae)
2007
International audience; Most aphids develop a cyclic parthenogenesis life-cycle. After several generations of viviparous parthenogenetic females, it follows a single annual generation of sexual individuals, usually in autumn, that mate and lay the sexual eggs. Shortening of photoperiod at the end of the summer is a key factor inducing the sexual response. With the survey here reported we aimed at identifying a collection of candidate genes to participate at some point in the cascade of events that lead to the sexual phenotypes. Following a suppression subtractive hybridization methodology (SSH) on the model aphid Acyrthosiphon pisum, we built and characterised two reciprocal cDNA libraries …
The Spodoptera exigua ABCC2 Acts as a Cry1A Receptor Independently of its Nucleotide Binding Domain II
2019
ABC proteins are primary-active transporters that require the binding and hydrolysis of ATP to transport substrates across the membrane. Since the first report of an ABCC2 transporter as receptor of Cry1A toxins, the number of ABC transporters known to be involved in the mode of action of Cry toxins has increased. In Spodoptera exigua, a mutation in the SeABCC2 gene is described as genetically linked to resistance to the Bt-product XentariTM. This mutation affects an intracellular domain involved in ATP binding, but not the extracellular loops. We analyzed whether this mutation affects the role of the SeABCC2 as a functional receptor to Cry1A toxins. The results show that Sf21 cells express…
Cytological and molecular characterization of three gametoclones of Citrus clementina
2013
Abstract Background Three gametoclonal plants of Citrus clementina Hort. ex Tan., cv. Nules, designated ESP, FRA, and ITA (derived from three labs in Spain, France, and Italy, respectively), were selected for cytological and molecular characterization in order to elucidate genomic rearrangements provoked by haploidization. The study included comparisons of their ploidy, homozygosity, genome integrity, and gene dosage, using chromosome counting, flow cytometry, SSR marker genotyping, and array-Comparative Genomic Hybridization (array-CGH). Results Chromosome counting and flow cytometry revealed that ESP and FRA were haploid, but ITA was tri-haploid. Homozygous patterns, represented by a sing…