Search results for "granules"

Electron microscopic immunogold cytochemistry reveals chromogranin A confined to secretory granules of porcine Merkel cells

1990

By ultrastructural immunohistochemistry using the immunogold technique, immunoreactive (ir) Chromogranin A (CGA) was found to be confined to the secretory vesicles of porcine Merkel cells. CGA was present predominantly in the periphery of the electron-dense core and on the clear halo. These findings indicate that CGA is a regular constituent of Merkel cell secretory granules but probably not exclusively responsible for their electron opacity.

Mixed Epidermal Cell Lymphocyte Reaction: HLA-DR+ Cells Exhibit a Greater Immunostimulatory Activity than CD1a+ Cells

1995

Induction and expression of immunity depends upon processing and presentation of antigens to T-cells by bone marrow derived HLA-DR+ antigen presenting cells (APC). In the induction of immunity to cutaneous antigens, the initial stages of this process begin within the skin itself, and both epidermis and dermis contain bone-marrow derived cells that have the capability of processing and presenting antigens. In normal epidermis, HLA-DR expression is believed to be confined to Langerhans cells with Birbeck granules and indeterminate cells without Birbeck granules. In diseased skin, particularly the inflammatory dermatoses, HLA-DR is commonly expressed by keratinocytes and has been related to th…

Biochemical characteristics of the soluble organic matrix from the shell of three recent terebratulid brachiopod species.

2008

7 pages; International audience; To build their shells, brachiopods secrete a mixture of proteins and polysaccharides, collectively called the organic matrix. This matrix mediates the calcification process by allowing crystal nucleation followed by elongation and finally by stopping the crystal growth. Ultimately, the matrix controls the different microstructures formed. Brachiopod shells are composites with both organic and inorganic constituents intimately associated. This work represents an attempt to characterize the matrices using two combined approaches: (1) scanning electron microscopy for localization within the shell, (2) analysing extracted matrices at the molecular level to deter…

GRANULAR-INFORMATION-BASED RISK ANALYSIS IN UNCERTAIN SITUATIONS

2006

In the real life almost all of the decisions that we have to make incorporate uncertainty about the future events. Assessment of the uncertainty and, thus, the risk that is inherent in these decisions models can be critical. It is even truer if we are talking about the possibility of negative impact on the environment. It is very important to assess all the environmental risks in a project if there is any hazard to the environment. In this paper the possibility of using granular information is considered. The main advantage of the granular information is that it can be used to assess risks in situations when information about future events is incomplete and imprecise. Moreover, we can use n…

ALS-RELATED FUS PROTEIN IS MISLOCALIZED TO CYTOPLASM AND RECRUITED INTO STRESS GRANULES IN FIBROBLASTS OF ASYMPTOMATIC FUS P525L MUTATION CARRIERS

Symptoms onset in Amyotrophic Lateral Sclerosis (ALS) occur when over 70% of motor neurons is already lost, suggesting a relatively long pre-symptomatic phase. The description of several genes linked to ALS (e.g., SOD1, FUS, TARDP, C9orf72) has now allowed identification of pre-symptomatic carriers. These pre-symptomatic (or even preclinical) carriers can be followed up with the aim to identify the very early clinical disease-related changes or a valuable biomarker. These efforts seem at present the best approach for the implementation of an early symptomatic therapy or for the disease prevention. In this work, we studied the expression of FUS protein in cultured skin fibroblasts from pre-s…